1.无锡商业职业技术学院 汽车技术学院，江苏 无锡 214153
2.盐城工学院 材料科学与工程学院，江苏 盐城 224002
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Lu LU, Lü XU, Xin ZHANG, et al. Fluorescent Detection for Cysteine/Homocysteine Using Hg2+ Modulated Nitrogen and Phosphorus Co-doped Carbon Nanodots. [J]. Chinese Journal of Luminescence 43(3):440-452(2022)
Lu LU, Lü XU, Xin ZHANG, et al. Fluorescent Detection for Cysteine/Homocysteine Using Hg2+ Modulated Nitrogen and Phosphorus Co-doped Carbon Nanodots. [J]. Chinese Journal of Luminescence 43(3):440-452(2022) DOI： 10.37188/CJL.20210389.
以叶酸和N-(膦酰基甲基)亚氨基二乙酸为原料，制备了具有丰富氮含量(10.0%)和高荧光量子产率(23.64%)的N,P-CDs。由于Hg,2+,与N, P-CDs之间的电子转移作用，N,P-CDs的荧光将被猝灭，基于此构建了半胱氨酸/同型半胱氨酸(Cys/Hcy)增强型纳米探针。利用Hg,2+,和硫醇基团之间更强的相互作用，实现Cys/Hcy的检测。Cys的测定在0.1~150 μmol/L范围内存在线性关系，Hcy的测定在0.1~100 μmol/L范围内存在线性关系，检测限分别为30 nmol/L和50 nmol/L。此外，本文所制备的N,P-CDs具有较强的抗基质干扰能力，可用于自来水和尿液等真实样品检测；因其超低的细胞毒性和出色的细胞渗透性，可用于检测活细胞内的Hg,2+,/Cys，在生物传感方面具有较为广阔的应用前景。
A new kind of N,P-CDs with rich nitrogen content(10.0%) and high fluorescence quantum yield(23.64%) was successfully prepared using folic acid and N-(phosphonomethyl)iminodiacetic acid as precursors. Due to the charge transfer between Hg,2+, and N,P-CDs, the fluorescence of N,P-CDs was quenched. Based on this, a cysteine/homocysteine(Cys/Hcy) enhanced nanoprobe was constructed using the stronger interaction between Hg,2+, and -SH. Linear relationships were established within the range of 0.1-150 μmol/L for Cys and 0.1-100 μmol/L for Hcy, respectively. The limit detections of Cys and Hcy were 30 nmol/L and 50 nmol/L. In addition, the as-prepared N,P-CDs with strong anti-matrix interference ability could be used for real samples such as tap water and urine. N,P-CDs were applied to sensing Hg,2+,/Cys within living cells favorably based on their ultralow cytotoxicity and outstanding cell permeability, indicating the potential applications in bioimaging and biosensing.
N P-CDsfluorescent probescysteine detectionhomocysteine detectioncell image
MILLS E L, RYAN D G, PRAG H A, et al. Itaconate is an anti-inflammatory metabolite that activates Nrf2 via alkylation of KEAP1 [J]. Nature, 2018, 556(7699):113-117.
SONG Y, LUO Y N, ZHU C Z, et al. Recent advances in electrochemical biosensors based on graphene two-dimensional nanomaterials [J]. Biosens. Bioelectron., 2016, 76:195-212.
ZHU N S, GUO X L, PANG S R, et al. Mitochondria-immobilized unimolecular fluorescent probe for multiplexing imaging of living cancer cells [J]. Anal. Chem., 2020, 92(16):11103-11110.
XIE X X, PENG Z Q, HUA X Y, et al. Selectively monitoring glutathione in human serum and growth-associated living cells using gold nanoclusters [J]. Biosens. Bioelectron., 2020, 148:111829-1-11.
VAN LEEUWEN K A, NARDIN T, BARKER D, et al. A novel LC-HRMS method reveals cysteinyl and glutathionyl polysulfides in wine [J]. Talanta, 2020, 218:121105-1-9.
IVANOV A V, VIRUS E D, LUZYANIN B P, et al. Capillary electrophoresis coupled with 1,1'-thiocarbonyldiimidazole derivatization for the rapid detection of total homocysteine and cysteine in human plasma [J]. J. Chromatogr. B, 2015, 1004:30-36.
LIU L R, ZHU G B, ZENG W, et al. Silicon quantum dot-coated onto gold nanoparticles as an optical probe for colorimetric and fluorometric determination of cysteine [J]. Microchim. Acta, 2019, 186(2):98-1-9.
CHEN S H, HUANG Z Z, JIA Q. Electrostatically confined in-situ preparation of stable glutathione-capped copper nanoclusters for fluorescence detection of lysozyme [J]. Sens. Actuators B:Chem., 2020, 319:128305-1-8.
LI K B, QU W B, SHEN Q X, et al. 1,8-Naphthalimide-based dual-response fluorescent probe for highly discriminating detection of cys and H2S [J]. Dyes Pigm., 2020, 173:107918-1-6.
WANG Q, ZHOU M, ZHANG L. A dual mode photoelectrochemical sensor for nitrobenzene and L-cysteine based on 3D flower-like Cu2SnS3@SnS2 double interfacial heterojunction photoelectrode [J]. J. Hazard. Mater., 2020, 382:121026-1-10.
WU N T, YU A W, ZHANG L B, et al. Biocompatible nanoplatform based on mussel adhesive chemistry:effective assembly,dual mode sensing,and cellular imaging performance [J]. Adv. Mater. Interfaces, 2019, 6(17):1900732-1-10.
QIAO G X, LIU L, HAO X X, et al. Signal transduction from small particles:sulfur nanodots featuring mercury sensing,cell entry mechanism and in vitro tracking performance [J]. Chem. Eng. J., 2020, 382:122907.
WANG Z X, JIN X, GAO Y F, et al. Fluorometric and colorimetric determination of hypochlorite using carbon nanodots doped with boron and nitrogen [J]. Microchim. Acta, 2019, 186(6):328-1-9.
YANG F, HE X, WANG C X, et al. Controllable and eco-friendly synthesis of P-riched carbon quantum dots and its application for copper (Ⅱ) ion sensing [J]. Appl. Surf. Sci., 2018, 448:589-598.
WANG Z X, YU X H, LI F, et al. Multiplexed ratiometric photoluminescent detection of pyrophosphate using anisotropic boron-doped nitrogen-rich carbon rugby ball-like nanodots [J]. J. Mater. Chem. B, 2018, 6(12):1771-1781.
JIAO Y, GAO Y F, MENG Y Y, et al. One-step synthesis of label-free ratiometric fluorescence carbon dots for the detection of silver ions and glutathione and cellular imaging applications [J]. ACS Appl. Mater. Interfaces, 2019, 11(18):16822-16829.
QU J, ZHANG X, LIU Y F, et al. N,P-co-doped carbon dots as a dual-mode colorimetric/ratiometric fluorescent sensor for formaldehyde and cell imaging via an aminal reaction-induced aggregation process [J]. Microchim. Acta, 2020, 187(6):355-1-10.
ATCHUDAN R, EDISON T N J I, PERUMAL S, et al. Hydrophilic nitrogen-doped carbon dots from biowaste using dwarf banana peel for environmental and biological applications [J]. Fuel, 2020, 275:117821-1-10.
LI Y S, ZHAO L C, LI B L, et al. Evaluating nitrite content changes in some Chinese home cooking with a newely-developed CDs diazotization spectrophotometry [J]. Food Chem., 2020, 330:127151.
LI M, LI X N, JIANG M Y, et al. Engineering a ratiometric fluorescent sensor membrane containing carbon dots for efficient fluoride detection and removal [J]. Chem. Eng. J., 2020, 399:125741.
SUN D, LIU T T, WANG C F, et al. Hydrothermal synthesis of fluorescent carbon dots from gardenia fruit for sensitive on-off-on detection of Hg2+ and cysteine [J]. Spectrochim. Acta, Part A, 2020, 240:118598-1-8.
ZHANG C, LIU M L, LI T T, et al. One-pot hydrothermal synthesis of dual-emission fluorescent carbon dots for hypochlorous acid detection [J]. Dyes Pigm., 2020, 180:108507-1-7.
LI G J, GUAN Y H, YE F Y, et al. Cyanine-based fluorescent indicator for mercury ion and bioimaging application in living cells [J]. Spectrochim. Acta Part A, 2020, 239:118465-1-6.
KUMAR A, KUMAR S, CHAE P S. A novel anthrapyridone diamine-based probe for selective and distinctive Cu2+ and Hg2+ sensing in aqueous solution; utility as molecular logic gates [J]. Dyes Pigm., 2020, 181:108522-1-14.
PAJEWSKA-SZMYT M, BUSZEWSKI B, GADZAŁA-KOPCIUCH R. Carbon dots as rapid assays for detection of mercury(Ⅱ) ions based on turn-off mode and breast milk [J]. Spectrochim. Acta Part A, 2020, 236:118320-1-8.
SUN Y C, WANG L Z, ZHOU J H, et al. A new phenothiazine-based fluorescence sensor for imaging Hg2+ in living cells [J]. Appl. Organomet. Chem., 2020, 34(11):e5945-1-11.
YE Z G, ZHANG Y H, LI G X, et al. Fluorescent determination of mercury(Ⅱ) by green carbon quantum dots synthesized from eggshell membrane [J]. Anal. Lett., 2020, 53(18):2841-2853.
PAN Z X, XU Z X, CHEN J, et al. Coumarin thiourea-based fluorescent turn-on Hg2+ probe that can be utilized in a broad pH range http://html.publish.founderss.cn/rc-pub/api/common/picture?pictureId=25657629&type=http://html.publish.founderss.cn/rc-pub/api/common/picture?pictureId=25657635&type=http://html.publish.founderss.cn/rc-pub/api/common/picture?pictureId=25657632&type=11 [J]. J. Fluoresc., 2020, 30(3):505-514.
GHOSH T, CHATTERJEE S, BHAYANI K, et al. A natural cyanobacterial protein C-phycoerythrin as an Hg2+ selective fluorescent probe in aqueous systems [J]. New J. Chem., 2020, 44(16):6601-6609.
CHU H T, YAO D, CHEN J Q, et al. Double-emission ratiometric fluorescent sensors composed of rare-earth-doped ZnS quantum dots for Hg2+ detection [J]. ACS Omega, 2020, 5(16):9558-9565.
REN H X, HUO F J, ZHANG Y B, et al. An NIR ESIPT-based fluorescent probe with large stokes shift for specific detection of Cys and its bioimaging in cells and mice [J]. Sens. Actuators B:Chem., 2020, 319:128248-1-8.
ZHOU P C, SHE M Y, LIU P, et al. Measuring the distribution and concentration of cysteine by fluorescent sensor for the visual study of paracetamol-induced pro-sarcopenic effect [J]. Sens. Actuators B: Chem., 2020, 318:128258.
ZHONG K L, ZHOU S Y, YAN X M, et al. A novel D-π-A type NBD-based fluorescent probe for ultrafast and distinguishable detection of Hcy/Cys and its bioimaging application [J]. J. Lumin., 2020, 224:117330.
JIANG X Q, HUANG J B, CHEN T Y, et al. Synthesis of hemicellulose/deep eutectic solvent based carbon quantum dots for ultrasensitive detection of Ag+ and L-cysteine with “off-on” pattern [J]. Int. J. Biol. Macromol., 2020, 153:412-420.
YUAN C L, QIN X, XU Y J, et al. Carbon quantum dots originated from chicken blood as peroxidase mimics for colorimetric detection of biothiols [J]. J. Photochem. Photobiol. A: Chem., 2020, 396:112529.
LIU C Y, MIAO Y Q, ZHANG X J, et al. Colorimetric determination of cysteine by a paper-based assay system using aspartic acid modified gold nanoparticles [J]. Microchim. Acta, 2020, 187(6):362-1-8.
SUN Z B, HUA Y, GAO M J, et al. Highly selective fluorescent 4-(4-(diethylamino)-2-hydroxystyryl)-1-methylpyridine iodide and nitrobenzofurazan based probe for cysteine with application in living cells [J]. Anal. Lett., 2021, 54(3):417-429.
鲁诗言, 于淑娟, 陈国全, 等. 氮、磷掺杂碳点的合成及在Pd2+传感中的应用 [J]. 发光学报, 2021, 42(1):53-60.
LU S Y, YU S J, CHEN G Q, et al. Synthesis of nitrogen and phosphorus doped carbon dots and their application in Pd2+ sensing [J]. Chin. J. Lumin., 2021, 42(1):53-60. (in Chinese)
HUANG S, YANG E L, YAO J D, et al. Red emission nitrogen,boron,sulfur co-doped carbon dots for “on-off-on” fluorescent mode detection of Ag+ ions and L-cysteine in complex biological fluids and living cells [J]. Anal. Chim. Acta, 2018, 1035:192-202.
FARHADIAN S, SHAREGHI B, SABOURY A A, et al. Molecular aspects of the interaction of spermidine and α-chymotrypsin [J]. Int. J. Biol. Macromol., 2016, 92:523-532.
王宁, 王新旭, 杨海芬, 等. 基于氮磷共掺杂碳点的荧光探针检测汞离子 [J]. 化学研究与应用, 2019, 31(3):440-448.
WANG N, WANG X X, YANG H F, et al. A fluorescent probe for determination of mercury ion based on phosphorus and nitrogen co-doped carbon dot [J]. Chem. Res. Appl., 2019, 31(3):440-448. (in Chinese)