Fluorescent Detection for Cysteine/Homocysteine Using Hg<sup>2+</sup> Modulated Nitrogen and Phosphorus Co-doped Carbon Nanodots

Lu LU; Lü XU; Xin ZHANG; Xiao-hong ZHOU; Le-ping YU

doi:10.37188/CJL.20210389

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Fluorescent Detection for Cysteine/Homocysteine Using Hg²⁺ Modulated Nitrogen and Phosphorus Co-doped Carbon Nanodots

Luminescence Applications and Interdisciplinary Fields | 更新时间：2022-04-07

- Fluorescent Detection for Cysteine/Homocysteine Using Hg²⁺ Modulated Nitrogen and Phosphorus Co-doped Carbon Nanodots
  增强出版
- Chinese Journal of Luminescence Vol. 43, Issue 3, Pages: 440-452(2022)
- 作者机构：
  
  1.无锡商业职业技术学院　汽车技术学院，江苏　无锡　214153
  2.盐城工学院　材料科学与工程学院，江苏　盐城　224002
- 作者简介：
- 基金信息：
  
  Jiangsu Province Natural Science Foundation(BK20191041);Natural Science Foundation of the Jiangsu Higher Education Institutions of China(19KJB480011);Science Foundation of Wuxi Vocational Institute of Commerce school project(KJXJ21604);Production and Education Integration Project of Wuxi Vocational Institute of Commerce school project(XQ202113)
- DOI：10.37188/CJL.20210389
  CLC：
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Lu LU, Lü XU, Xin ZHANG, et al. Fluorescent Detection for Cysteine/Homocysteine Using Hg²⁺ Modulated Nitrogen and Phosphorus Co-doped Carbon Nanodots. [J]. Chinese Journal of Luminescence 43(3):440-452(2022)
DOI：

Lu LU, Lü XU, Xin ZHANG, et al. Fluorescent Detection for Cysteine/Homocysteine Using Hg²⁺ Modulated Nitrogen and Phosphorus Co-doped Carbon Nanodots. [J]. Chinese Journal of Luminescence 43(3):440-452(2022) DOI： 10.37188/CJL.20210389.

摘要

以叶酸和N-(膦酰基甲基)亚氨基二乙酸为原料，制备了具有丰富氮含量(10.0%)和高荧光量子产率(23.64%)的N,P-CDs。由于Hg,2+,与N, P-CDs之间的电子转移作用，N,P-CDs的荧光将被猝灭，基于此构建了半胱氨酸/同型半胱氨酸(Cys/Hcy)增强型纳米探针。利用Hg,2+,和硫醇基团之间更强的相互作用，实现Cys/Hcy的检测。Cys的测定在0.1~150 μmol/L范围内存在线性关系，Hcy的测定在0.1~100 μmol/L范围内存在线性关系，检测限分别为30 nmol/L和50 nmol/L。此外，本文所制备的N,P-CDs具有较强的抗基质干扰能力，可用于自来水和尿液等真实样品检测；因其超低的细胞毒性和出色的细胞渗透性，可用于检测活细胞内的Hg,2+,/Cys，在生物传感方面具有较为广阔的应用前景。

Abstract

A new kind of N,P-CDs with rich nitrogen content(10.0%) and high fluorescence quantum yield(23.64%) was successfully prepared using folic acid and N-(phosphonomethyl)iminodiacetic acid as precursors. Due to the charge transfer between Hg,2+, and N,P-CDs, the fluorescence of N,P-CDs was quenched. Based on this, a cysteine/homocysteine(Cys/Hcy) enhanced nanoprobe was constructed using the stronger interaction between Hg,2+, and -SH. Linear relationships were established within the range of 0.1-150 μmol/L for Cys and 0.1-100 μmol/L for Hcy, respectively. The limit detections of Cys and Hcy were 30 nmol/L and 50 nmol/L. In addition, the as-prepared N,P-CDs with strong anti-matrix interference ability could be used for real samples such as tap water and urine. N,P-CDs were applied to sensing Hg,2+,/Cys within living cells favorably based on their ultralow cytotoxicity and outstanding cell permeability, indicating the potential applications in bioimaging and biosensing.

关键词

NP-CDs荧光探针半胱氨酸检测同型半胱氨酸检测细胞成像

Keywords

N P-CDsfluorescent probescysteine detectionhomocysteine detectioncell image

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Fluorescent Detection for Cysteine/Homocysteine Using Hg2+ Modulated Nitrogen and Phosphorus Co-doped Carbon Nanodots

DOI：10.37188/CJL.20210389

摘要

Abstract

关键词

Keywords

references

Fluorescent Detection for Cysteine/Homocysteine Using Hg²⁺ Modulated Nitrogen and Phosphorus Co-doped Carbon Nanodots