基于信号增强的电化学发光适配体传感器检测三磷酸腺苷

姚武; 崔朋; 胡晓倩

doi:10.3788/fgxb20204106.0744

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基于信号增强的电化学发光适配体传感器检测三磷酸腺苷

发光学应用及交叉前沿 | 更新时间：2020-09-15

- 基于信号增强的电化学发光适配体传感器检测三磷酸腺苷
- Electrochemiluminescent Aptasensor Based on Signal Enhancement for Determination of Adenosine Triphosphate
- 发光学报 2020年41卷第6期页码：744-752
- 作者机构：
  
  1.黄山学院化学化工学院, 安徽黄山 245041
  2.黄山学院生命与环境科学学院, 安徽黄山 245041
- 作者简介：
  
  [ "姚武(1968-), 男, 安徽黄山人, 博士, 教授, 2009年于安徽师范大学获得博士学位, 主要从事药物分析和生物传感器方面的研究。E-mail:yaowu92@sohu.com" ]
- 基金信息：
  
  安徽省自然科学基金面上项目(11040606M41);安徽省高校自然科学重点研究项目(KJ2016A683)
- DOI：10.3788/fgxb20204106.0744
  中图分类号： O657
- 纸质出版日期：2020-6，
  
  收稿日期：2020-3-24，
  
  录用日期：2020-4-16
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姚武, 崔朋, 胡晓倩. 基于信号增强的电化学发光适配体传感器检测三磷酸腺苷[J]. 发光学报, 2020,41(6):744-752.

Wu YAO, Peng CUI, Xiao-qian HU. Electrochemiluminescent Aptasensor Based on Signal Enhancement for Determination of Adenosine Triphosphate[J]. Chinese Journal of Luminescence, 2020,41(6):744-752.
姚武, 崔朋, 胡晓倩. 基于信号增强的电化学发光适配体传感器检测三磷酸腺苷[J]. 发光学报, 2020,41(6):744-752. DOI： 10.3788/fgxb20204106.0744.

Wu YAO, Peng CUI, Xiao-qian HU. Electrochemiluminescent Aptasensor Based on Signal Enhancement for Determination of Adenosine Triphosphate[J]. Chinese Journal of Luminescence, 2020,41(6):744-752. DOI： 10.3788/fgxb20204106.0744.

摘要

基于三磷酸腺苷（ATP）适配体与ATP分子作用后可以显著增强电化学发光信号的性能，研究了一种用于ATP含量检测的电化学发光适配体（ECL-aptamer）传感器。通过电沉积方法获得纳米金电极。3'端标记联吡啶钌发光分子的探针DNA通过5'端修饰的巯基自组装到纳米金电极表面，然后与5'端标记二茂铁分子的ATP核酸适配体互补杂交，形成刚性线形的双链DNA，由此构建的传感器产生较弱的电化学发光（ECL）信号。该传感器在ATP溶液中孵化后，由于ATP分子与ATP适配体强的特异性结合，使得适配体分子与探针DNA分子解离，从电极表面脱落进入溶液，此时电极表面的探针DNA在强电解质溶液中可以形成发卡型的茎环结构，产生显著增强的ECL信号。ECL信号强度与ATP浓度的对数值呈线性关系，线性范围为10.0~1.0×10

pmol/L，相关系数

=0.995 9，检测限为5.0 pmol/L。该传感器的灵敏度与检测范围高于目前已报道的结果，显示出了ATP检测的应用潜力。

Abstract

Based on the property that the electrochemiluminescence (ECL) signal could be significantly enhanced after adenosine triphosphate (ATP) aptamers interacted with ATP molecules

an ECL aptasensor was developed to detect ATP. A nano-Au electrode was obtained by electrodeposition method. The probe DNA with 3'-terminal labeled by ruthenium complex was self-assembled to the surface of the nano-Au electrode with the sulfhydryl group modified on the 5'-terminal

and then hybridized with the ATP aptamer which 5'-terminal was labeled by ferrocene molecule

to form rigid linear double-stranded DNA. Thus

the constructed sensor produced weak ECL signal. After incubation in an ATP solution

the aptamer molecules could dissociate from the probe DNA and fall off from the electrode surface due to the strong specific binding of ATP molecules and ATP aptamer

and the probe DNA on the electrode surface could form hairpin like stem ring structure

resulting in a stronger ECL signal. The linear range of ECL intensity and the logarithm of the ATP concentration was 10.0~1.0×10

pmol/L with a detection limit of 5.0 pmol/L

and the correlation coefficient was 0.995 9. The sensitivity and detection range of the sensor are higher than those reported so far

which shows the potential application of ATP detection.

关键词

电化学发光适配体生物传感器三磷酸腺苷

Keywords

electrochemiluminescenceaptamerbiosensoradenosine triphosphate

references

KRICKA L J, STANLEY P E. Electrochemiluminescence:1996-1998[J].Luminescence, 1999, 14(2):113-118.

RICHTER M M. Electrochemiluminescence (ECL)[J].Chem. Rev., 2004, 104(6):3003-3036.

TUERK C, GOLD L. Systematic evolution of ligands by exponential enrichment:RNA ligands to bacteriophage T4 DNA polymerase[J].Science, 1990, 249(4968):505-510.

YANG Q, GOLDSTEIN I J, MEI H Y, et al.. DNA ligands that bind tightly and selectively to cellobiose[J].Proc. Natl. Acad. Sci. USA, 1998, 95(10):5462-5467.

杜斌, 童朝阳, 刘志伟, 等.基于适配体-表面等离子共振的生物传感技术及应用[J].发光学报, 2017, 38(8):1039-1046.

DU B, TONG Z Y, LIU Z W, et al.. Research and application of biosensing technology based on aptamer-surface plasmon resonance[J].Chin. J. Lumin., 2017, 38(8):1039-1046. (in Chinese)

NIE Y M, YUAN X D, ZHANG P, et al.. Versatile and ultrasensitive electrochemiluminescence biosensor for biomarker detection based on nonenzymatic amplification and aptamer-triggered emitter release[J].Anal. Chem., 2019, 91(5):3452-3458.

JIANG X Y, WANG H J, WANG H J, et al.. Electrochemiluminescence biosensor based on 3-D DNA nanomachine signal probe powered by protein-aptamer binding complex for ultrasensitive mucin 1 detection[J].Anal. Chem., 2017, 89(7):4280-4286.

WU M S, YUAN D J, XU J J, et al.. Sensitive electrochemiluminescence biosensor based on Au-ITO hybrid bipolar electrode amplification system for cell surface protein detection[J].Anal. Chem., 2013, 85(24):11960-11965.

JIANG X Y, WANG H J, WANG H J, et al.. Signal-switchable electrochemiluminescence system coupled with target recycling amplification strategy for sensitive mercury ion and mucin 1 assay[J].Anal. Chem., 2016, 88(18):9243-9250.

HAN Z L, SHU J N, LIANG X, et al.. Label-free ratiometric electrochemiluminescence aptasensor based on nanographene oxide wrapped titanium dioxide nanoparticles with potential-resolved electrochemiluminescence[J].Anal. Chem., 2019, 91(19):12260-12267.

XIA H, LI L L, YIN Z Y, et al.. Biobar-coded gold nanoparticles and DNAzyme-based dual signal amplification strategy for ultrasensitive detection of protein by electrochemiluminescence[J].ACS Appl. Mater. Interfaces, 2015, 7(1):696-703.

SHAO K, WANG B R, YE S Y, et al.. Signal-amplified near-infrared ratiometric electrochemiluminescence aptasensor based on multiple quenching and enhancement effect of graphene/gold nanorods/G-quadruplex[J].Anal. Chem., 2016, 88(16):8179-8187.

LIU J Q, HE D G, LIU Q Q, et al.. Vertically ordered mesoporous silica film-assisted label-free and universal electrochemiluminescence aptasensor platform[J].Anal. Chem., 2016, 88(23):11707-11713.

WANG C Q, QIAN J, WANG K, et al.. Nitrogen-doped graphene quantum dots@SiO2 nanoparticles as electrochemiluminescence and fluorescence signal indicators for magnetically controlled aptasensor with dual detection channels[J].ACS Appl. Mater. Interfaces, 2015, 7(48):26865-26873.

GE J J, ZHAO Y, LI C L, et al.. Versatile electrochemiluminescence and electrochemical "On-Off" assays of methyltransferases and aflatoxin B1 based on a novel multifunctional DNA nanotube[J].Anal. Chem., 2019, 91(5):3546-3554.

ZHAO M, CHEN A Y, HUANG D, et al.. MoS2 quantum dots as new electrochemiluminescence emitters for ultrasensitive bioanalysis of lipopolysaccharide[J].Anal. Chem., 2017, 89(16):8335-8342.

GUO Y S, SHANG X X, LIU F, et al.. Novel enhancer for luminol-AuNP electrochemiluminescence and decoration on RNA membranes for effective cytosensing[J].ACS Appl. Bio Mater., 2018, 1(5):1647-1655.

JIE G F, WANG L, YUAN J X, et al.. Versatile electrochemiluminescence assays for cancer cells based on dendrimer/CdSe-ZnS-quantum dot nanoclusters[J].Anal. Chem., 2011, 83(10):3873-3880.

ZHOU B, QIU Y Y, WEN Q Q, et al.. Dual electrochemiluminescence signal system for in situ and simultaneous evaluation of multiple cell-surface receptors[J].ACS Appl. Mater. Interfaces, 2017, 9(3):2074-2082.

SHI H W, ZHAO W, LIU Z, et al.. Temporal sensing platform based on bipolar electrode for the ultrasensitive detection of cancer cells[J].Anal. Chem., 2016, 88(17):8795-8801.

GAO H F, WANG X F, LI M, et al.. Ultrasensitive electrochemiluminescence aptasensor for assessment of protein heterogeneity in small cell population[J].ACS Appl. Bio Mater., 2019, 2(7):3052-3058.

LEI Y M, HUANG W X, ZHAO M,et al.. Electrochemiluminescence resonance energy transfer system:mechanism and application in ratiometric aptasensor for lead ion[J].Anal. Chem., 2015, 87(15):7787-7794.

HU L Z, BIAN Z, LI H J, et al..[Ru(bpy)2dppz]2+ electrochemiluminescence switch and its applications for DNA interaction study and label-free ATP aptasensor[J].Anal. Chem., 2009, 81(23):9807-9811.

LIU Y T, LEI J P, HUANG Y, et al.. "Off-On" electrochemiluminescence system for sensitive detection of ATP via target-induced structure switching[J].Anal. Chem., 2014, 86(17):8735-8741.

YAO W, WANG L, WANG H Y, et al.. An aptamer-based electrochemiluminescent biosensor for ATP detection[J].Biosens. Bioelectron., 2009, 24(11):3269-3274.

YAO W, WANG L, WANG H Y, et al.. An electrochemiluminescent DNA sensor based on nano-gold enhancement and ferrocene quenching[J].Biosens. Bioelectron., 2013, 40(1):356-361.

ZHANG B Z, WEI C Y. The sensitive detection of ATP and ADA based on turn-on fluorescent copper/silver nanoclusters[J].Anal. Bioanal. Chem., 2020, 412(11):2529-2536.

XUE N, WU S J, LI Z B, et al.. Ultrasensitive and label-free detection of ATP by using gold nanorods coupled with enzyme assisted target recycling amplification[J].Anal. Chim. Acta, 2020, 1104:117-124.

LI X, YANG J M, XIE J Q, et al.. Cascaded signal amplification via target-triggered formation of aptazyme for sensitive electrochemical detection of ATP[J].Biosens. Bioelectron., 2018, 102:296-300.

WU L, ZHANG X H, LIU W, et al.. Sensitive electrochemical aptasensor by coupling "signal-on" and "signal-off" strategies[J].Anal. Chem., 2013, 85(17):8397-8402.

HUANG Y, LEI J P, CHENG Y, et al.. Target-assistant Zn2+-dependent DNAzyme for signal-on electrochemiluminescent biosensing[J].Electrochim. Acta, 2015, 155:341-347.

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