一种基于异硫氰酸荧光素的pH纳米传感器制备
Preparation of Fluorescent pH Nanosensor Based on Flourescein Isothiocyanate
- 发光学报 2020年41卷第6期 页码：729-733
- 作者机构：
1.北京邮电大学电子工程学院 安全生产智能监控北京市重点实验室, 北京 100876
- 作者简介：
[ "杨步高(1999-), 男, 江西抚州人, 在读本科生, 主要从事发光纳米材料的研究。E-mail:1766638540@qq.com" ]
[ "王小卉(1987-), 女, 山东烟台人, 博士, 副教授, 2015年于北京交通大学获得博士学位, 主要从事发光纳米材料和生物荧光分析领域的研究。E-mail:wangxiaohui@bupt.edu.cn" ]
- 基金信息：国家自然科学基金(61605014)
DOI：10.3788/fgxb20204106.0729
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杨步高, 赵一, 韦晓菲, 等. 一种基于异硫氰酸荧光素的pH纳米传感器制备[J]. 发光学报, 2020,41(6):729-733.
Bu-gao YANG, Yi ZHAO, Xiao-fei WEI, et al. Preparation of Fluorescent pH Nanosensor Based on Flourescein Isothiocyanate[J]. Chinese Journal of Luminescence, 2020,41(6):729-733.
杨步高, 赵一, 韦晓菲, 等. 一种基于异硫氰酸荧光素的pH纳米传感器制备[J]. 发光学报, 2020,41(6):729-733. DOI： 10.3788/fgxb20204106.0729.
Bu-gao YANG, Yi ZHAO, Xiao-fei WEI, et al. Preparation of Fluorescent pH Nanosensor Based on Flourescein Isothiocyanate[J]. Chinese Journal of Luminescence, 2020,41(6):729-733. DOI： 10.3788/fgxb20204106.0729.
摘要
无创且实时检测微观环境的pH变化在医疗等领域具有重要的研究价值，本文通过灵活简单的共沉淀方法制备了一种基于pH敏感分子异硫氰酸荧光素（FITC）的荧光pH纳米传感器，FITC的荧光强度随pH值增加发生明显变化，当pH值从3变至9时，荧光强度增大约38倍，基于FITC荧光强度变化可实现对pH的灵敏检测，p,K,a值为6.07。该荧光pH纳米传感器具有小粒径、高灵敏度、良好的可逆性和生物相容性，在细胞等微环境pH检测方面将具有良好的应用前景。
Abstract
The non-invasive and real-time pH detection is of significant importance in biomedicine fields. In this paper, a fluorescent pH nanosensor based on flourescein isothiocyanate(FITC) is prepared by a facile coprecipiation method. The fluorescence intensity of FITC changes obviously with the increase of pH. When the pH changes from 3 to 9, the fluorescence intensity of FITC increases up to about 38 times. The pH detection can be realized by measuring fluorescence intensity changes of FITC. The value of p,K,a is calculated to be 6.07. The fluorescent pH nanosensor has small particle size, high sensitivity, good reversibility and excellent biocompatibility, which is promising in intracellular pH detection.
关键词
异硫氰酸荧光素pH检测纳米颗粒荧光传感
Keywords
flourescein isothiocyanatepH detectionnanoparticlefluorescence sensing
references
KNEIPP J, KNEIPP H, WITTIG B, et al.. Novel optical nanosensors for probing and imaging live cells[J].Nanomed.:Nanotechnol., Biol. Med., 2010, 6(2):214-226.
ISLAM S, BIDIN N, RIAZ S, et al.. Sol-gel based fiber optic pH nanosensor:structural and sensing properties[J].Sens. Actuators A Phys., 2016, 238:8-18.
WANG X D, STOLWIJK J A, LANG T, et al.. Ultra-small, highly stable, and sensitive dual nanosensors for imaging intracellular oxygen and pH in cytosol[J].J. Am. Chem. Soc., 2012, 134(41):17011-17014.
FUJISAKU T, TANABE R, ONODA S, et al.. pH nanosensor using electronic spins in diamond[J].ACS Nano, 2019, 13(10):11726-11732.
SHAMSIPUR M, BARATI A, NEMATIFAR Z. Fluorescent pH nanosensors:design strategies and applications[J].J. Photochem. Photobiol. C Photochem. Rev., 2019, 39:76-141.
WENCEL D, ABEL T, MCDONAGH C. Optical chemical pH sensors[J].Anal. Chem., 2014, 86(1):15-29.
PENG H S, STOLWIJK J A, SUN L N, et al.. A nanogel for ratiometric fluorescent sensing of intracellular pH values[J].Angew. Chem. Int. Ed., 2010, 49(25):4246-4249.
LU H G, JIN Y G, TIAN Y Q, et al.. New ratiometric optical oxygen and pH dual sensors with three emission colors for measuring photosynthetic activity in cyanobacteria[J].J. Mater. Chem., 2011, 21(48):19293-19301.
SCHREML S, MEIER R J, WOLFBEIS O S, et al.. 2D luminescence imaging of pH in vivo[J].Proc. Natl. Acad. Sci. USA, 2011, 108(6):2432-2437.
WANG X D, MEIER R J, WOLFBEIS O S. A fluorophore-doped polymer nanomaterial for referenced imaging of pH and temperature with sub-micrometer resolution[J].Adv. Funct. Mater., 2012, 22(20):4202-4207.
WANG C, OTTO S, DORN M, et al.. Luminescent TOP nanosensors for simultaneously measuring temperature, oxygen, and pH at a single excitation wavelength[J].Anal. Chem., 2019, 91(3):2337-2344.
WANG X H, PENG H S, YANG L, et al.. Targetable phosphorescent oxygen nanosensors for the assessment of tumor mitochondrial dysfunction by monitoring the respiratory activity[J].Angew. Chem. Int. Ed., 2014, 53(46):12471-12475.
SHI W, LI X H, MA H M. Fluorescent probes and nanoparticles for intracellular sensing of pH values[J].Methods Appl. Fluoresc., 2014, 2(4):042001.
MA T C, MA Y, LIU S J, et al.. Dye-conjugated upconversion nanoparticles for ratiometric imaging of intracellular pH values[J].J. Mater. Chem. C, 2015, 3(26):6616-6620.
NÄREOJA T, DEGUCHI T, CHRIST S, et al.. Ratiometric sensing and imaging of intracellular pH using polyethylenimine-coated photon upconversion nanoprobes[J].Anal. Chem., 2017, 89(3):1501-1508.
BRUNI F, PEDRINI J, BOSSIO C, et al.. Two-color emitting colloidal nanocrystals as single-particle ratiometric probes of intracellular pH[J].Adv. Funct. Mater., 2017, 27(12):1605533-1-9.
FULAZ S, HIEBNER D, BARROS C H N, et al.. Ratiometric imaging of the in situ pH distribution of biofilms by use of fluorescent mesoporous silica nanosensors[J].ACS Appl. Mater. Interfaces, 2019, 11(36):32679-32688.
WANG X H, YU Y X, CHENG K, et al.. Polylysine modified conjugated polymer nanoparticles loaded with the singlet oxygen probe 1, 3-diphenylisobenzofuran and the photosensitizer indocyanine green for use in fluorometric sensing and in photodynamic therapy[J].Microchim. Acta, 2019, 186(12):842-1-8.
WANG X H, PENG H S, YANG W, et al.. Indocyanine green-platinum porphyrins integrated conjugated polymer hybrid nanoparticles for near-infrared-triggered photothermal and two-photon photodynamic therapy[J].J. Mater. Chem. B, 2017, 5(9):1856-1862.
GE J Y, FAN L, ZHANG K, et al.. A two-photon ratiometric fluorescent probe for effective monitoring of lysosomal pH in live cells and cancer tissues[J].Sens. Actuators B Chem., 2018, 262:913-921.
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