柠檬黄与玉米醇溶蛋白的相互作用研究

饶震红; 王明安; 陈蓁蓁; 张莉

doi:10.3788/fgxb20194004.0511

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柠檬黄与玉米醇溶蛋白的相互作用研究

发光学应用及交叉前沿 | 更新时间：2020-08-12

- 柠檬黄与玉米醇溶蛋白的相互作用研究
- Interaction Between Tartrazine and Zein
- 发光学报 2019年40卷第4期页码：511-519
- 作者机构：
  
  1. 中国农业大学理学院北京,100083
  2. 山东师范大学化学化工与材料科学学院,山东济南,250014
- 作者简介：
- 基金信息：
  
  国家自然科学基金（21172254）资助项目
- DOI：10.3788/fgxb20194004.0511
  中图分类号： O657.3
- 纸质出版日期：2019-4-5，
  
  网络出版日期：2018-8-30，
  
  收稿日期：2018-6-8，
  
  修回日期：2018-7-31，
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饶震红, 王明安, 陈蓁蓁等. 柠檬黄与玉米醇溶蛋白的相互作用研究[J]. 发光学报, 2019,40(4): 511-519

RAO Zhen-hong, WANG Ming-an, CHEN Zhen-zhen etc. Interaction Between Tartrazine and Zein[J]. Chinese Journal of Luminescence, 2019,40(4): 511-519
饶震红, 王明安, 陈蓁蓁等. 柠檬黄与玉米醇溶蛋白的相互作用研究[J]. 发光学报, 2019,40(4): 511-519 DOI： 10.3788/fgxb20194004.0511.

RAO Zhen-hong, WANG Ming-an, CHEN Zhen-zhen etc. Interaction Between Tartrazine and Zein[J]. Chinese Journal of Luminescence, 2019,40(4): 511-519 DOI： 10.3788/fgxb20194004.0511.

摘要

为了对玉米醇溶蛋白的改性研究及柠檬黄色素的安全使用评估提供理论依据，借助于荧光光谱法、紫外光谱法、全内反射-傅里叶红外光谱（ATR-FTIR）自去卷积计算及核磁共振氢谱研究了人工合成色素柠檬黄对玉米醇溶蛋白构象的影响。结果表明，柠檬黄对玉米醇溶蛋白有明显的荧光猝灭作用，猝灭机理较复杂。柠檬黄可以与玉米醇溶蛋白结合，分子间主要以疏水作用力结合，利用Stern-Volmer方程和Van't Hoff 公式计算获得结合比为1:1，结合常数

值较大。通过FTIR的自去卷积计算分析，这种结合反应导致玉米蛋白二级结构中

螺旋结构、

折叠和

转角均发生显著改变。核磁共振氢谱分析表明由于玉米醇溶蛋白具有两亲性，当溶剂为二甲基亚砜时，混合溶液为低极性环境，化学位移略向高磁场移动；当溶剂为重水D

O时，化学位移明显地向低磁场移动，而且这种作用与反应时间长短无关。进一步说明在极性和低极性的环境下柠檬黄都会引起玉米醇溶蛋白构象的改变。

Abstract

The effect of tartrazine on the zein conformation was studied by fluorescence spectroscopy

UV spectroscopy

ATR-FTIR self-deconvolution calculations and

H NMR spectroscopy

in order to provide a theoretical basis for the modification study of zein and the safe use assessment of tartrazine. The results showed that tartrazine had obvious fluorescence quenching effect on zein

and the quenching mechanism was complicated. Tartrazine can bind to zein

and the molecules were mainly combined by hydrophobic interaction. Using the Stern-Volmer equation and Van't Hoff formula

their binding ratio was calculated to be 1:1 and the binding constant

value was larger. By self-deconvolution analysis of Fourier transform infrared spectroscopy

this binding reaction resulted in significant changes in the

-helix structure

-folding and

-turn angle in the secondary structure of zein. Nuclear magnetic resonance spectroscopy indicated that due to the amphipathic nature of zein

when the solvent was dimethyl sulfoxide

the mixed solution was in a low-polarity environment

the chemical shift shifted slightly to a high magnetic field; when the solvent was heavy water D

chemical shifts significantly shift to low magnetic fields

and this effect was independent of the length of reaction time. It was further illustrated that tartrazine can cause changes in the zein conformation in a polar and low polarity environment.

关键词

光谱分析构象变化柠檬黄玉米醇溶蛋白二级结构

Keywords

spectral analysisconformational changetartrazinezeinsecondary structure

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