银覆盖冠状硅柱阵列基底的制备、SERS特性分析及肿瘤标志物miRNA-106a的检测

洑颢; 佟丽莹; 梁照恒; 彭乐; 王福艳; 周骏

doi:10.3788/fgxb20194003.0317

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银覆盖冠状硅柱阵列基底的制备、SERS特性分析及肿瘤标志物miRNA-106a的检测

器件制备及器件物理 | 更新时间：2020-08-12

- 银覆盖冠状硅柱阵列基底的制备、SERS特性分析及肿瘤标志物miRNA-106a的检测
- Silver-covered Si Corona-pillar Array Substrate: Fabrication, SERS Characteristic Analysis and Detection of Tumor Marker miRNA-106a
- 发光学报 2019年40卷第3期页码：317-325
- 作者机构：
  
  1. 宁波大学理学院微电子科学与工程系,浙江宁波,315211
  2. 宁波大学医学院浙江省病理生理学重点实验室,浙江宁波,315211
- 作者简介：
- 基金信息：
  
  国家自然科学基金（61320106014，61675104，81501421）;宁波大学王宽诚幸福基金资助项目
- DOI：10.3788/fgxb20194003.0317
  中图分类号： O433.1
- 纸质出版日期：2019-3-5，
  
  网络出版日期：2018-8-23，
  
  收稿日期：2018-5-1，
  
  修回日期：2018-8-5，
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洑颢, 佟丽莹, 梁照恒等. 银覆盖冠状硅柱阵列基底的制备、SERS特性分析及肿瘤标志物miRNA-106a的检测[J]. 发光学报, 2019,40(3): 317-325

FU Hao, TONG Li-ying, LIANG Zhao-heng etc. Silver-covered Si Corona-pillar Array Substrate: Fabrication, SERS Characteristic Analysis and Detection of Tumor Marker miRNA-106a[J]. Chinese Journal of Luminescence, 2019,40(3): 317-325
洑颢, 佟丽莹, 梁照恒等. 银覆盖冠状硅柱阵列基底的制备、SERS特性分析及肿瘤标志物miRNA-106a的检测[J]. 发光学报, 2019,40(3): 317-325 DOI： 10.3788/fgxb20194003.0317.

FU Hao, TONG Li-ying, LIANG Zhao-heng etc. Silver-covered Si Corona-pillar Array Substrate: Fabrication, SERS Characteristic Analysis and Detection of Tumor Marker miRNA-106a[J]. Chinese Journal of Luminescence, 2019,40(3): 317-325 DOI： 10.3788/fgxb20194003.0317.

摘要

以聚苯乙烯（PS）小球为模板，采用金属辅助刻蚀和湿法化学刻蚀技术，制备大面积冠状硅柱阵列，再原位生长银纳米粒子后得到银覆盖冠状硅柱阵列（Ag/Si CPA）基底。实验表明，制备的基底具有优良的表面增强拉曼散射（SERS）特性，电磁增强因子达到1.8110

。同时，将制备的罗丹明分子（R6G）标记的DNA发卡探针与基底链接，在与miRNA-106a互补杂交后进行SERS信号检测，获得相应的剂量-响应曲线。结果表明，基于（Ag/Si CPA）基底的SERS特性，开展miRNA-106a的检测，具有特异性好和灵敏度高的优势，检测范围为1 fmolL

-1

~100 pmolL

-1

，检测极限为0.917 fmolL

-1

。此外，与实时荧光定量多聚核苷酸链式反应（RT-qPCR）方法相比，不仅检测结果一致，而且基于SERS光谱技术的检测方法具有更高的灵敏度。

Abstract

Polystyrene(PS) spheres were used as a template

and the metal-assisted etching and wet chemical etching techniques were applied to prepare large-area Si corona-pillar arrays

silver nanoparticles were then grown

in-situ

to obtain silver-covered Si corona-pillar array(Ag/Si CPA) substrate. The experimental results show that the prepared substrate has excellent surface enhanced Raman scattering(SERS) characteristics

and its electromagnetic enhancement factor is up to 1.8110

. Meanwhile

the rhodamine molecule(R6G)-labeled DNA hairpin probe was immobilized on the surface of Ag/Si CPA substrate

and SERS signal detection was performed for getting the corresponding dose-response curve after complementary hybridization with various concentration of microRNA-106a. The results demonstrate that the detection of miRNA-106a

based on the SERS characteristic of Ag/Si CPA substrate

exhibits the advantages of specificity and high sensitivity with the limit of detection of 0.917 fmolL

-1

in the detection range of 1 fmolL

-1

-100 pmolL

-1

. In addition

compared with the real-time fluorescence quantitative polynucleotide chain reaction(RT-qPCR) method

not only the detection results are consistent

but the detection method based on SERS spectroscopy has a higher sensitivity.

关键词

表面增强拉曼散射硅柱阵列发卡探针肿瘤标志物miRNA

Keywords

surface-enhanced Raman scatteringsi pillar arrayhairpin probetumor markermiRNA

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