荧光/表面增强拉曼散射双模式纳米光学探针的构建及性能研究

宿健; 张谷令; 彭洪尚

doi:10.3788/fgxb20183909.1323

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荧光/表面增强拉曼散射双模式纳米光学探针的构建及性能研究

发光学应用及交叉前沿 | 更新时间：2020-08-12

- 荧光/表面增强拉曼散射双模式纳米光学探针的构建及性能研究
- Preparation of A Dual-mode Optical Nanoprobe Based on Fluorescence and Surface Enhanced Raman Scattering
- 发光学报 2018年39卷第9期页码：1323-1329
- 作者机构：
  
  1. 中央民族大学生命与环境科学学院, 北京 100081
  2. 中央民族大学理学院, 北京 100081
- 作者简介：
- 基金信息：
  
  国家自然科学基金（61775245）资助项目()
- DOI：10.3788/fgxb20183909.1323
  中图分类号： O482.31
- 纸质出版日期：2018-9-5，
  
  网络出版日期：2018-5-9，
  
  收稿日期：2018-3-26，
  
  修回日期：2018-7-9，
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宿健, 张谷令, 彭洪尚. 荧光/表面增强拉曼散射双模式纳米光学探针的构建及性能研究[J]. 发光学报, 2018,39(9): 1323-1329

SU Jian, ZHANG Gu-ling, PENG Hong-shang. Preparation of A Dual-mode Optical Nanoprobe Based on Fluorescence and Surface Enhanced Raman Scattering[J]. Chinese Journal of Luminescence, 2018,39(9): 1323-1329
宿健, 张谷令, 彭洪尚. 荧光/表面增强拉曼散射双模式纳米光学探针的构建及性能研究[J]. 发光学报, 2018,39(9): 1323-1329 DOI： 10.3788/fgxb20183909.1323.

SU Jian, ZHANG Gu-ling, PENG Hong-shang. Preparation of A Dual-mode Optical Nanoprobe Based on Fluorescence and Surface Enhanced Raman Scattering[J]. Chinese Journal of Luminescence, 2018,39(9): 1323-1329 DOI： 10.3788/fgxb20183909.1323.

摘要

提出一种新型的荧光及表面增强拉曼散射（SERS）双模式光学纳米探针。首先，通过再沉淀-包覆法合成二氧化硅包覆香豆素6的纳米颗粒，再在二氧化硅表面静电吸附多聚赖氨酸分子形成包覆层，随后通过原位还原的方法在多聚赖氨酸壳层复合银纳米颗粒，最后在银纳米颗粒表面吸附拉曼分子即形成双模式纳米探针。该探针通过二氧化硅包覆的荧光分子产生荧光信号，以多聚赖氨酸表面的银纳米颗粒作为SERS增强基底，利用拉曼分子获得SERS信号，实现了荧光及SERS双模式成像。荧光与表面增强拉曼散射相结合的双模式分析技术可同时发挥二者的优点，提高成像的分辨率和灵敏度，在生物医学领域具有重要的应用价值。

Abstract

A novel type of dual-mode optical nanoprobe combining fluorescence and surface enhanced Raman scattering was described. Firstly

the fluorescent nanoparticles doped with coumarin 6(C6) and coated with silica were prepared by a reprecipitation-encapsulation method. Then

PLL molecules were electrostatically absorbed onto the surface of negatively charged NPs. Subsequently

the silver nanoparticles were attached to the surface of PLL shell by the

in-situ

reduction. Finally

Raman molecules were connected on the silver nanoparticles surface. The fluorescent signal in this probe derives from the fluorescent molecular C6. The silver nanoparticles are used as the enhanced substrate of SERS

and the SERS signal is from Raman molecules. The double-mode analysis technique

combining fluorescence and surface enhanced Raman scattering

possesses high resolution and sensitivity of imaging

and is promising in the biomedical field.

关键词

荧光表面增强拉曼银纳米颗粒纳米探针

Keywords

fluorescencesurface enhanced Raman scatteringsilver nanoparticlenanoprobe

references

ZONG S F, WANG Z Y, YANG J, et al.. A SERS and fluorescence dual mode cancer cell targeting probe based on silica coated Au@Ag core-shell nanorods[J]. Talanta, 2012, 97:368-375.

ZONG S F, WANG Z Y, ZHANG R H, et al.. A multiplex and straightforward aqueous phase immunoassay protocol through the combination of SERS-fluorescence dual mode nanoprobes and magnetic nanobeads[J]. Biosens. Bioelectron., 2013, 41:745-751.

WANG Z Y, ZONG S F, YANG J, et al.. Dual-mode probe based on mesoporous silica coated gold nanorods for targeting cancer cells[J]. Biosens. Bioelectron., 2011, 26:2883-2889.

WANG Z Y, ZONG S F, CHEN H, et al.. Silica coated gold nanoaggregates prepared by reverse microemusion method:dual mode probes for multiplex immunoassay using SERS and fluorescence[J]. Talanta, 2011, 86:170-177.

KIN K, LEE Y M, LEE H B, et al.. Silver-coated silica beads applicable as core materials of dual-tagging sensors operating via SERS and MEF[J]. ACS Appl. Mater. Interf., 2009, 1(10):2174-2180.

YU K N, LEE S M, HAN J Y, et al.. Multiplex targeting, tracking, and imaging of apoptosis by fluorescent surface enhanced Raman spectroscopic dots[J]. Bioconjug. Chem., 2007, 18:1155-1162.

LEE S M. Dual-modal silica nanoprobes with surface enhanced Raman spectroscopic and fluorescent signals. International Conference on Nano-Bio Sensing, Imaging, and Spectroscopy, Jeju, Korea, 2015, 9523:952309.

KIM K, LEE H B, CHOI J Y, et al.. Silver-coated dye-embedded silica beads:a core material of dual tagging sensors based on fluorescence and Raman scattering[J]. ACS Appl. Mater. Interf., 2011, 3:324-330.

PING J T, PENG H S, DUAN W B, et al.. Synthesis and optimization of ZnPc-loaded biocompatible nanoparticles for efficient photodynamic therapy[J]. J. Mater. Chem. B, 2016, 4(25):4482-4489.

HE D G, HE X X, WANG K M, et al.. A facile route for shape-selective synthesis of silica nanostructures using poly-l-lysine as template[J]. Chin. Chem. Lett., 2013, 24(2):99-102.

LEE H, DELLATORE S M, MILLER W M, et al.. Mussel-inspired surface chemistry for multifunctional coatings[J]. Science, 2007, 318:426-430.

WANGOO N, BHASIN K K, MEHTA S K, et al.. Synthesis and capping of water-dispersed gold nanoparticles by an amino acid:bioconjugation and binding studies[J]. J. Colloid. Interf. Sci., 2008, 323:247-254.

PEI Y, XIAO C, GOH T W, et al.. Tuning surface properties of amino-functionalized silica for metal nanoparticle loading:the vital role of an annealing process[J]. Surf. Sci., 2016, 648:299-306.

SOLOVYEVA E V, UBYIVOVK E V, DENISOVA A S, et al.. Effect of diaminostilbene as a molecular linker on Ag nanoparticles:SERS study of aggregation and interparticle hot spots in various environments[J]. Colloids Surf. A:Physicochem. Eng. Aspects, 2018, 538:542-548.

BOAZBOU NEWMAI M, VERMA M, SENTHIL KUMAR P, et al.. Monomer functionalized silica coated with Ag nanoparticles for enhanced SERS hotspots[J]. Appl. Surf. Sci., 2018, 440:133-143.

YANG M, YU J, LEI F, et al.. Synthesis of low-cost 3D-porous ZnO/Ag SERS-active substrate with ultrasensitive and repeatable detectability[J]. Sens. Actuators B:Chem., 2018, 256:268-275.

WANG X H, PENG H S, ZHANG Z, et al.. Synthesis of ratiometric fluorescent nanoparticles for sensing oxygen[J]. Microchim. Acta, 2012, 178(1-2):147-152.

WANG X H, PENG H S, DING H, et al.. Biocompatible fluorescent core-shell nanoparticles for ratiometric oxygen sensing[J]. J. Mater. Chem., 2012, 22:16066-16071.

SIKDER M, LEAD J R, CHANDLER G T, et al.. A rapid approach for measuring silver nanoparticle concentration and dissolution in seawater by UV-Vis[J]. Sci. Total Environm., 2018, 618:597-607.

ELLIS L J, BAALOUSHA M, VALSAMI J M, et al.. Seasonal variability of natural water chemistry affects the fate and behaviour of silver nanoparticles[J]. Chemosphere, 2018, 191:616-625.

RAJAM K S, RANI M E, GUNASEELI R, et al.. Extracellular synthesis of silver nanoparticles by the fungus Emericella nidulans EV4 and its application[J]. Indian J. Experiment. Biol., 2017, 55:262-265.

MANIRAJ A, KUMAR S M, KANNAN M. Optimization and characterization of green synthesized silver nanoparticles and its inhibitory activity against biofilm forming bacterial pathogens[J]. J. Adv. Appl. Sci. Res., 2017, 1(9):97-106.

AKIN S T, LIU X, DUNCAN M A. Laser synthesis and spectroscopy of acetonitrile/silver nanoparticles[J]. Chem. Phys. Lett., 2015, 640:161-164.

YOUNG J J, CHENG K M, YOUNG Y A, et al.. Chondroitin sulfate-stabilized silver nanoparticles:improved synthesis and their catalytic, antimicrobial, and biocompatible activities[J]. Carbohydrate Res., 2018, 457:14-24.

DU Y, FANG Y. Assignment of charge transfer absorption band in optical absorption spectra of the adsorbate-silver colloid system[J]. Spectrochim. Acta Part A:Mol. Biomol. Spectrosc., 2004, 60:535-539.

ZHANG A, ZHANG J. Quenching and enhancing of SERS of methyl orange after the addition of chlorine and nitrate anions[J]. Physica B:Condensed Matter, 2009, 404:2435-2438.

HILDEBRANDT P, STOCKBURGER M. Surface-enhanced resonance raman spectroscopy of Rhodamine 6G adsorbed on colloidal silver[J]. J. Phys. Chem., 1984, 88(24):5935-5944.

RIGO M V, SEO J, KIM W J, et al.. Plasmon coupling of R6G-linked gold nanoparticle assemblies for surface-enhanced Raman spectroscopy[J]. Vibrat. Spectrosc., 2011, 57:315-318.

GUO Q Q, MA X F, YU X, et al.. Green synthesis and formation mechanism of Ag nanoflowers using l-cysteine and the assessment of Ag nanoflowers as SERS substrates[J]. Colloids Surf. A:Physicochem. Eng. Aspects, 2017, 530:33-37.

VAN DYCK C, FU B, VAN DUYNE R P, et al.. Deducing the adsorption geometry of Rhodamine 6G from the surface-induced mode renormalization in surface-enhanced Raman spectroscopy[J]. J. Phys. Chem. C, 2017, 122:465-473.

CHEN S, LI X, GUO Y, et al.. A Ag-molecularly imprinted polymer composite for efficient surface-enhanced Raman scattering activities under a low-energy laser[J]. Analyst, 2015, 140:3239-3243.

WEI W, HUANG Q. Rapid fabrication of silver nanoparticle-coated filter paper as SERS substrate for low-abundance molecules detection[J]. Spectrochim. Acta A:Mol. Biomol. Spectrosc., 2017, 179:211-215.

ZHANG L, LI P, LUO L, et al.. Sensitive detection of Rhodamine B in condiments using surface-enhanced resonance Raman scattering (SERRS) silver nanowires as substrate[J]. Appl. Spectrosc., 2017, 71:2395-2403.

FUKUI T, NAIKI H, MASUO S. In situ observation of surface-enhanced Raman scattering from silver nanoparticle dimers and trimers fabricated using atomic force microscopy manipulation[J]. J. Phys. Chem. C, 2017, 121:19329-19333.

GOLOVIN A V, POLUBOTKO A M. Manifestation of a strong quadrupole interaction and peculiarities in the SERS and SEHRS spectra of 4,4'-bipyridine[J]. Phys. Solid State, 2017, 59:1368-1376.

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