两亲性壳聚糖基聚合物碳点的合成及其在载药方面的应用

于淑娟; 陈宽; 汪丰; 朱永飞

doi:10.3788/fgxb20183907.0915

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两亲性壳聚糖基聚合物碳点的合成及其在载药方面的应用

材料合成及性能 | 更新时间：2020-08-12

- 两亲性壳聚糖基聚合物碳点的合成及其在载药方面的应用
- Synthesis of Amphiphilic Polymer Carbon Dots Fluorescence Materials from Octyl Chitosan and Their Applications in Drug Delivery
- 发光学报 2018年39卷第7期页码：915-922
- 作者机构：
  
  广西师范学院化学与材料科学学院, 广西南宁 530001
- 作者简介：
- 基金信息：
  
  广西自然科学基金（2016GXNSFAA380203）资助项目()
- DOI：10.3788/fgxb20183907.0915
  中图分类号： TB383;O613.71
- 纸质出版日期：2018-7-5，
  
  网络出版日期：2018-3-23，
  
  收稿日期：2017-10-21，
  
  修回日期：2018-2-28，
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于淑娟, 陈宽, 汪丰等. 两亲性壳聚糖基聚合物碳点的合成及其在载药方面的应用[J]. 发光学报, 2018,39(7): 915-922

YU Shu-juan, CHEN Kuan, WANG Feng etc. Synthesis of Amphiphilic Polymer Carbon Dots Fluorescence Materials from Octyl Chitosan and Their Applications in Drug Delivery[J]. Chinese Journal of Luminescence, 2018,39(7): 915-922
于淑娟, 陈宽, 汪丰等. 两亲性壳聚糖基聚合物碳点的合成及其在载药方面的应用[J]. 发光学报, 2018,39(7): 915-922 DOI： 10.3788/fgxb20183907.0915.

YU Shu-juan, CHEN Kuan, WANG Feng etc. Synthesis of Amphiphilic Polymer Carbon Dots Fluorescence Materials from Octyl Chitosan and Their Applications in Drug Delivery[J]. Chinese Journal of Luminescence, 2018,39(7): 915-922 DOI： 10.3788/fgxb20183907.0915.

摘要

通过水热法合成了系列具有高荧光量子产率（42.9%）辛基化壳聚糖基两亲性聚合物碳点荧光材料。利用红外光谱、紫外吸收光谱、光电子能谱、透射电镜、X射线衍射及荧光光谱对聚合物碳点进行了表征。以阿霉素为模型药物，研究了聚合物碳点对阿霉素的载药性能。当辛基取代度为76.42%时，其最大载药量和包封率分别为49.6%与47.4%。在磷酸盐缓冲液中，载药纳米胶束呈前期快速释放，后期缓慢释放的双相特征。将载药纳米胶束与鼻咽癌细胞作用，发现其存活率随着载药纳米胶束加入量的增加而降低，说明该纳米胶束对鼻咽癌细胞有一定的抑制作用。总之，该聚合物碳点材料在药物载体与荧光示踪方面有潜在的应用价值。

Abstract

Fluorescent materials P(CS-g-OC-CA)Ds with high fluorescence quantum yield(42.9%) were prepared by hydrothermal method using chitosan

citric acid

Octylaldehyde and N-(2-hydroxyethyl) ethylenediamine as raw materials. The P(CS-g-OC-CA)Ds were characterized by infrared spectroscopy

ultraviolet spectroscopy (UV)

X-ray powder diffraction

X-ray photoelectron spectroscopy

transmission electron microscopy and photoluminescence spectra. The drug loading properties of the P(CS-g-OC-CA)Ds were studied using doxorubicin (DOX) as a model drug. When the degree of octane substitution is 76.42%

the optimal loading and entrapment efficiency of DOX are 49.6% and 47.4%

respectively. Notably

the amount and encapsulation efficiency of DOX can be controlled by controlling the degree of octane substitution. After loading

the micelles are approximately 80-90 nm in diameter. The fluid mechanics particle size is 114.3 nm

and the surface potential is +5.72 mV.

In vitro

release studies demonstrate a dual release phase from the drug-loaded micelles in pH 7.4 phosphate buffer

with a rapid first-phase release and a slow second-phase release of DOX. Cytotoxicity studies demonstrate that there is no obvious cytotoxicity of blank P(CS-g-OC-CA)Ds

and the survival rate of nasopharyngeal carcinoma (CNE-2) cells decreases with the increasing of the amount of drug-loaded micelles. These results show that the nanomicelles have a strong inhibitory effect on CNE-2 cells. In conclusion

the obtained P(CS-g-OC-CA)Ds are potentially useful as drug-delivery and fluorescence trace systems.

关键词

壳聚糖聚合物碳点荧光材料载药阿霉素

Keywords

chitosanpolymer carbon dotfluorescence materialdrug loadingDOX

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