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山西师范大学 生命科学院,山西 临汾,041000
纸质出版日期:2016-1-10,
收稿日期:2015-10-11,
修回日期:2015-11-13,
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司芳瑞, 苗艳明, 杨茂青等. 基于MPA包覆的Mn掺杂ZnS量子点/米托蒽醌复合体系对DNA的检测[J]. 发光学报, 2016,37(1): 13-21
SI Fang-rui, MIAO Yan-ming, YANG Mao-qing etc. Detection of DNA Based on Manganese-doped ZnS Quantum Dots/MTX Composite System[J]. Chinese Journal of Luminescence, 2016,37(1): 13-21
司芳瑞, 苗艳明, 杨茂青等. 基于MPA包覆的Mn掺杂ZnS量子点/米托蒽醌复合体系对DNA的检测[J]. 发光学报, 2016,37(1): 13-21 DOI: 10.3788/fgxb20163701.0013.
SI Fang-rui, MIAO Yan-ming, YANG Mao-qing etc. Detection of DNA Based on Manganese-doped ZnS Quantum Dots/MTX Composite System[J]. Chinese Journal of Luminescence, 2016,37(1): 13-21 DOI: 10.3788/fgxb20163701.0013.
利用3-羟基丙酸(MPA)包覆的Mn掺杂ZnS量子点与盐酸米托蒽醌(MTX)构建了一种可简单测定DNA的磷光复合体系。该体系以MTX作为良好的电子受体
通过光诱导电子转移(PIET)原理猝灭Mn掺杂ZnS量子点的室温磷光(RTP)。当体系中加入DNA后
DNA通过静电和嵌插作用与MTX结合
形成更稳定的复合物
并使MTX从Mn掺杂ZnS量子点表面脱离
Mn掺杂ZnS量子点的RTP恢复
从而实现了对DNA的痕量检测。该体系检测DNA的线性范围为0.1~20 mgL
-1
检出限为0.07 mgL
-1
。该方法可有效避免其他共存物质的干扰并可应用于实际生物样品中DNA含量的快捷测定。
A simple phosphorescent compound system for DNA detection was established by utilizing Mn (covered with 3-hydracrylicacid (MPA)) doped with ZnS quantum dots and mitoxantrone. By taking MTX as good electron acceptor
the room temperature phosphorescence (RTP) of Mn-doped ZnS quantum dots were quenched through photoinduced electron transfer principle. After the addition of DNA in system
DNA and MTX were combined through static electricity and intercalation
and a more stable compound was formed as well
which enable MTX removing from the surface of Mn-doped ZnS quantum dots and achieving RTP recovery of Mn-doped ZnS quantum dots. Thus
the trace detection of DNA was realized. The linear scope of this system for DNA detection and the detection limit is 0.1-20 mgL
-1
and 0.07 mgL
-1
respectively. This method can effectively avoid the interference from other coexisting substances
which can be used in rapid detection of DNA content in practical biological sample.
量子点室温磷光检测DNA盐酸米托蒽醌
quantum dotsphosphorescencedetectionDNAmitoxantrone
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