壳聚糖包裹AgInS2的物理表征及生物毒性研究

张晓凡; 刘丽炜; 邹鹏; 胡思怡; 王玥; 张喜和

doi:10.3788/fgxb20153610.1118

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壳聚糖包裹AgInS₂的物理表征及生物毒性研究

材料合成及性能 | 更新时间：2020-08-12

- 壳聚糖包裹AgInS₂的物理表征及生物毒性研究
- Physical Characterization and Biological Toxicity of Chitosan-capped AgInS₂ Quantum Dots
- 发光学报 2015年36卷第10期页码：1118-1125
- 作者机构：
  
  长春理工大学理学院,吉林长春,130022
- 作者简介：
- 基金信息：
  
  国家自然科学基金(11204020)();吉林省国际纳米光子学与生物光子学重点实验室支撑项目(20140622009JC)();国防预研究基金(62201070711)
- DOI：10.3788/fgxb20153610.1118
  中图分类号： O482.31
- 纸质出版日期：2015-10-10，
  
  收稿日期：2015-6-15，
  
  修回日期：2015-8-12，
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张晓凡, 刘丽炜, 邹鹏等. 壳聚糖包裹AgInS2的物理表征及生物毒性研究[J]. 发光学报, 2015,36(10): 1118-1125

ZHANG Xiao-fan, LIU Li-wei, ZOU Peng etc. Physical Characterization and Biological Toxicity of Chitosan-capped AgInS2 Quantum Dots[J]. Chinese Journal of Luminescence, 2015,36(10): 1118-1125
张晓凡, 刘丽炜, 邹鹏等. 壳聚糖包裹AgInS2的物理表征及生物毒性研究[J]. 发光学报, 2015,36(10): 1118-1125 DOI： 10.3788/fgxb20153610.1118.

ZHANG Xiao-fan, LIU Li-wei, ZOU Peng etc. Physical Characterization and Biological Toxicity of Chitosan-capped AgInS2 Quantum Dots[J]. Chinese Journal of Luminescence, 2015,36(10): 1118-1125 DOI： 10.3788/fgxb20153610.1118.

摘要

选用天然多糖中唯一的碱性多糖壳聚糖作为稳定剂和包裹剂

成功合成了水溶性的AgInS

量子点/低分子量壳聚糖纳米复合材料(AgInS

/LCSMS)。利用透射电子显微镜(TEM)、FT-IR傅里叶红外光谱仪、紫外吸收光谱、荧光分光光度计等表征手段对纳米复合材料的形貌、化学组成及光学性质进行了研究。结果表明

AgInS

/LCSMS纳米复合材料的粒径约为5~6 nm

在水相中仍具有较稳定的发光。之后

对AgInS

/LCSMS纳米复合材料的生物相容性进行了研究

对比AgInS

/LCSMS纳米复合材料与AgInS

量子点的细胞活性测试结果发现

AgInS

/LCSMS纳米复合材料的细胞活性比AgInS

量子点有了明显的提高

说明通过低分子量壳聚糖的包裹可以明显提高纳米材料的生物相容性。因此

这类具有较好水溶性和生物相容性的荧光 AgInS

/LCSMS纳米复合材料可作为优良的生物荧光标记材料在生物医学检验、细胞以及活体成像研究中有广泛的应用前景。

Abstract

The water-soluble AgInS

quantum dots/low molecular weight chitosan nanocomposites (AgInS

/LCSMS) were successfully synthesized. Natural polysaccharide in alkaline polysaccharideChitosan was used as stabilizer and capped agent. The structure

chemical composition

and optical properties of AgInS

/LCSMS nanocomposites were studied by transmission electron microscopy (TEM)

Fourier transform infrared spectroscopy (FT-IR)

fluorescence microscope

fluorescence spectrophotometer

and UV-Vis absorption spectroscopy

etc

. The results show that the particle diameter of AgInS

/LCSMS nanocomposites is about 5-6 nm

and they are still have a relatively stable luminescence in the water phase. In addition

we studied the biological compatibility of the AgInS

/LCSMS nanocomposites. Compared with pure AgInS

quantum dots

the cell activity of AgInS

/LCSMS nanocomposites has an obvious improvement. It shows that low molecular weight chitosan wrapping can obviously improve the biocompatibility of the nanometer materials. Therefore

this kind of AgInS

/LCSMS fluorescent nanocomposites with good water solubility and biological compatibility show significant value for potential applications in biomedicine test

biolabel

cell and

in vivo

imaging studies.

关键词

低分子量壳聚糖AgInS2量子点毒性

Keywords

low molecular weight chitosanAgInS2quantum dotstoxicity

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