荧光碳点在脊椎类模式动物斑马鱼中的活体成像与毒理学研究

邓帅; 李雨珊; 段延芳; 李丽; 安利民; 郑敏; 李炳生; 吴琼; 孙再成

doi:10.3788/fgxb20153604.0485

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荧光碳点在脊椎类模式动物斑马鱼中的活体成像与毒理学研究

发光学应用及交叉前沿 | 更新时间：2020-08-12

- 荧光碳点在脊椎类模式动物斑马鱼中的活体成像与毒理学研究
- Flourescent Imaging and Toxicology Study of Carbon Dots in Transparent Zebrafishes
- 发光学报 2015年36卷第4期页码：485-490
- 作者机构：
  
  1. 发光学及应用国家重点实验室中国科学院长春光学精密机械与物理研究所,吉林长春,130033
  2. 哈尔滨工业大学生命科学与技术学院,黑龙江哈尔滨,150080
  3. 黑龙江大学物理科学与技术学院,黑龙江哈尔滨,150080
  4. 哈尔滨工业大学基础与交叉科学研究院,黑龙江哈尔滨,150080
- 作者简介：
- 基金信息：
  
  国家自然科学基金(21403061,11474076)();黑龙江省博士后科研启动基金(LBH-Q13082)();中国博士后科学基金(2013M541420)();黑
- DOI：10.3788/fgxb20153604.0485
  中图分类号： O482.31
- 纸质出版日期：2015-4-3，
  
  收稿日期：2015-1-5，
  
  修回日期：2015-2-7，
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邓帅, 李雨珊, 段延芳等. 荧光碳点在脊椎类模式动物斑马鱼中的活体成像与毒理学研究[J]. 发光学报, 2015,36(4): 485-490

DENG Shuai, LI Yu-shan, DUAN Yan-fang etc. Flourescent Imaging and Toxicology Study of Carbon Dots in Transparent Zebrafishes[J]. Chinese Journal of Luminescence, 2015,36(4): 485-490
邓帅, 李雨珊, 段延芳等. 荧光碳点在脊椎类模式动物斑马鱼中的活体成像与毒理学研究[J]. 发光学报, 2015,36(4): 485-490 DOI： 10.3788/fgxb20153604.0485.

DENG Shuai, LI Yu-shan, DUAN Yan-fang etc. Flourescent Imaging and Toxicology Study of Carbon Dots in Transparent Zebrafishes[J]. Chinese Journal of Luminescence, 2015,36(4): 485-490 DOI： 10.3788/fgxb20153604.0485.

摘要

将荧光碳点引入透明斑马鱼胚胎中

研究了荧光碳点在斑马鱼中的活体成像和对斑马鱼的毒副作用。通过对水中添加荧光碳纳米颗粒

从形态上观察其对于斑马鱼个体发育的生物学效应

实时观察其在斑马鱼体内富集代谢的情况

为荧光碳点及其复合物在生物活体成像方面的研究应用奠定基础。结果表明

荧光碳点对斑马鱼生长发育无明显的毒副作用

不会导致胚胎发育异常(通过观察尾巴弯曲情况、色素深浅、血管发育速度、鱼鳔发育速度来确定对胚胎发育的影响)

并且对斑马鱼无明显致死现象。荧光碳点进入斑马鱼体内的时间非常迅速

并且可以在48 h之内经代谢排出体外。

Abstract

In this paper

we introduce the carbon dots (CDs) into transparent embryos of zebrafishes and study the fluorescent imaging and toxicology of CDs. With immerging oosperms and infants of zebrafishes into the aqueous solution of CDs for 1.5 h

the CDs go through the cell membrane and emerge inside of them. Then

from the morphological

we study the biological effects on the individual development and

in vivo

observe the supersession of zebrafishes. We demonstrate that CDs have no apparent toxicity on the growing development and do not induce the development abnormity of embryo. Furthermore

the CDs do not lead to the death of zebrafishes. We also find that CDs can enter into the zebrafisher rapidly and be expelled from the body within 48 h.

关键词

斑马鱼荧光碳点胚胎发育生物毒性

Keywords

zebrafishescarbon dotsembryonic developmentbiotoxicity

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