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山西师范大学 生命学院,山西 临汾,041000
纸质出版日期:2015-4-3,
收稿日期:2015-1-28,
修回日期:2015-2-22,
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杨茂青, 苗艳明, 闫桂琴. 基于Mn掺杂ZnS量子点-BSA纳米复合材料检测头孢曲松钠[J]. 发光学报, 2015,36(4): 472-479
YANG Mao-qing, MIAO Yan-ming, YAN Gui-qin. Detection of Ceftriaxone Based on ZnS:Mn Quantum Dots-BSA Nanohybrids[J]. Chinese Journal of Luminescence, 2015,36(4): 472-479
杨茂青, 苗艳明, 闫桂琴. 基于Mn掺杂ZnS量子点-BSA纳米复合材料检测头孢曲松钠[J]. 发光学报, 2015,36(4): 472-479 DOI: 10.3788/fgxb20153604.0472.
YANG Mao-qing, MIAO Yan-ming, YAN Gui-qin. Detection of Ceftriaxone Based on ZnS:Mn Quantum Dots-BSA Nanohybrids[J]. Chinese Journal of Luminescence, 2015,36(4): 472-479 DOI: 10.3788/fgxb20153604.0472.
以3-巯基丙酸(MPA)为稳定剂
采用水相合成法合成了具有优良光学性质的Mn掺杂ZnS量子点。该量子点在室温条件下即可发射较强的磷光信号。将量子点与牛血清蛋白(BSA)偶联后
形成了纳米复合材料。由于BSA对量子点表面缺陷进行了有效修复
量子点发出的磷光明显增强。当加入头孢曲松钠后
头孢曲松钠与BSA的相互作用使量子点的磷光被有效猝灭
该磷光猝灭量(△
P
)与头孢曲松钠的浓度在一定范围内呈良好的线性关系(
R
=0.99)。量子点与BSA偶联的最佳条件为:pH=7.4
反应温度37 ℃
反应时间40 min
BSA浓度80 mgL
-1
量子点浓度40 mgL
-1
。反应形成新的生物纳米复合材料
作为头孢曲松钠的磷光探针
其线性范围为0~30 molL
-1
相关系数
R
=0.99
检出限为0.14 molL
-1
相对标准偏差为1.63%。
Using 3-mercaptopropionic acid(MPA) as the stabilizer
ZnS:Mn quantum dots(QDs) were synthesized
via
water phase method
which could emit strong phosphorescence at room temperature. Bovine serum albumin (BSA) can effectively recover the defect on the surface of ZnS:Mn QDs
so the phosphorescence intensity of ZnS:Mn QDs can be enhanced after the conjugation between BSA and ZnS:Mn QDs. Ceftriaxone sodium can obviously quench the phosphorescence of ZnS:Mn QDs-BSA
and the quenched phosphorescence intensity amount (△
P
) is linearly proportional to the concentration of ceftriaxone sodium with the correlation coefficient
R
=0.99. The optimal condition of conjunction between ZnS:Mn QDs and BSA is as following: pH=7.4
reaction temperature of 37 ℃
response time of 40 min
BSA concentration of 80 mgL
-1
ZnS:Mn QDs concentration of 40 mgL
-1
respectively. As the phosphorescence probe
the synthesized ZnS:Mn QDs-BSA can detect ceftriaxone sodium effectively with the linear scope of 0-30 molL
-1
correlation coefficient
R
=0.99
inspection limit of 0.14 molL
-1
relative standard deviation of 1.63%
respectively.
量子点牛血清蛋白磷光探针头孢曲松钠
quantum dotsbovine serum albuminphosphorescence probeceftriaxone
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