基于Mn掺杂ZnS量子点-BSA纳米复合材料检测头孢曲松钠

杨茂青; 苗艳明; 闫桂琴

doi:10.3788/fgxb20153604.0472

您当前的位置：

首页 >

文章列表页 >

基于Mn掺杂ZnS量子点-BSA纳米复合材料检测头孢曲松钠

发光学应用及交叉前沿 | 更新时间：2020-08-12

- 基于Mn掺杂ZnS量子点-BSA纳米复合材料检测头孢曲松钠
- Detection of Ceftriaxone Based on ZnS:Mn Quantum Dots-BSA Nanohybrids
- 发光学报 2015年36卷第4期页码：472-479
- 作者机构：
  
  山西师范大学生命学院,山西临汾,041000
- 作者简介：
- 基金信息：
  
  山西省重点化学优势学科建设项目(912019)();国家教育部博士点联合基金(20111404110002)资助项目()
- DOI：10.3788/fgxb20153604.0472
  中图分类号： O657;O482.31
- 纸质出版日期：2015-4-3，
  
  收稿日期：2015-1-28，
  
  修回日期：2015-2-22，
扫描看全文
杨茂青, 苗艳明, 闫桂琴. 基于Mn掺杂ZnS量子点-BSA纳米复合材料检测头孢曲松钠[J]. 发光学报, 2015,36(4): 472-479

YANG Mao-qing, MIAO Yan-ming, YAN Gui-qin. Detection of Ceftriaxone Based on ZnS:Mn Quantum Dots-BSA Nanohybrids[J]. Chinese Journal of Luminescence, 2015,36(4): 472-479
杨茂青, 苗艳明, 闫桂琴. 基于Mn掺杂ZnS量子点-BSA纳米复合材料检测头孢曲松钠[J]. 发光学报, 2015,36(4): 472-479 DOI： 10.3788/fgxb20153604.0472.

YANG Mao-qing, MIAO Yan-ming, YAN Gui-qin. Detection of Ceftriaxone Based on ZnS:Mn Quantum Dots-BSA Nanohybrids[J]. Chinese Journal of Luminescence, 2015,36(4): 472-479 DOI： 10.3788/fgxb20153604.0472.

摘要

以3-巯基丙酸(MPA)为稳定剂

采用水相合成法合成了具有优良光学性质的Mn掺杂ZnS量子点。该量子点在室温条件下即可发射较强的磷光信号。将量子点与牛血清蛋白(BSA)偶联后

形成了纳米复合材料。由于BSA对量子点表面缺陷进行了有效修复

量子点发出的磷光明显增强。当加入头孢曲松钠后

头孢曲松钠与BSA的相互作用使量子点的磷光被有效猝灭

该磷光猝灭量(△

)与头孢曲松钠的浓度在一定范围内呈良好的线性关系(

=0.99)。量子点与BSA偶联的最佳条件为:pH=7.4

反应温度37 ℃

反应时间40 min

BSA浓度80 mgL

-1

量子点浓度40 mgL

-1

。反应形成新的生物纳米复合材料

作为头孢曲松钠的磷光探针

其线性范围为0~30 molL

-1

Abstract

Using 3-mercaptopropionic acid(MPA) as the stabilizer

ZnS:Mn quantum dots(QDs) were synthesized

via

water phase method

which could emit strong phosphorescence at room temperature. Bovine serum albumin (BSA) can effectively recover the defect on the surface of ZnS:Mn QDs

so the phosphorescence intensity of ZnS:Mn QDs can be enhanced after the conjugation between BSA and ZnS:Mn QDs. Ceftriaxone sodium can obviously quench the phosphorescence of ZnS:Mn QDs-BSA

and the quenched phosphorescence intensity amount (△

) is linearly proportional to the concentration of ceftriaxone sodium with the correlation coefficient

=0.99. The optimal condition of conjunction between ZnS:Mn QDs and BSA is as following: pH=7.4

reaction temperature of 37 ℃

response time of 40 min

BSA concentration of 80 mgL

-1

ZnS:Mn QDs concentration of 40 mgL

-1

respectively. As the phosphorescence probe

the synthesized ZnS:Mn QDs-BSA can detect ceftriaxone sodium effectively with the linear scope of 0－30 molL

-1

correlation coefficient

=0.99

inspection limit of 0.14 molL

-1

relative standard deviation of 1.63%

respectively.

关键词

量子点牛血清蛋白磷光探针头孢曲松钠

Keywords

quantum dotsbovine serum albuminphosphorescence probeceftriaxone

references

Miao Y M, Zhang Z F, Gong Y, et al. Phosphorescent quantum dots/doxorubicin nanohybrids based on photoinduced electron transfer for detection of DNA [J]. Biosens. Bioelectron., 2014, 59(15):300-306.

Tang N Y. Effect of strain on formation of antibonding hole ground states in InAs quantum dots [J]. Opt. Precision Eng. (光学精密工程), 2013, 21(6):1472-1478 (in Chinese).

Chen S H, Greeff A P, Swart H C. A comparative study between the simulated and measured cathodoluminescence generated in ZnS:Cu,Al,Au phosphor powder [J]. J. Lumin., 2005, 113:191-198.

Qin J J, Cao L X, Liu W, et al. ZnS:Mn/SiO2 quantum dots modified with PVP as fluorescent sensor for Pb2+ ions in sea water [J]. Chin. J. Lumin.(发光学报), 2014, 35(7):858-865 (in Chinese).

Zhang P F, Song J, Chen J, et al. Study on conjugation of quantum dot with anti-hepatitis B surface antigen antibody [J]. Chin. J. Anal. Chem.(分析化学), 2013, 41(6):846-850 (in Chinese).

Du H Y, Wei Z P, Li S, et al. Luminescent properties of surface modified ZnS:Mn quantum dot and detection of biological molecules [J]. Chin. J. Lumin.(发光学报), 2013, 34(4):421-426 (in Chinese).

Xie H Y, Pang D W. Preparation and application of quantum dot research progress in biological detection [J]. Chin. J. Anal. Chem.(分析化学), 2004, 32(8):1099-1103 (in Chinese).

Chen J, Sun J F, Guo L, et al. Mn-doped ZnS QDs at room temperature phosphorescence detection of lead ions [J]. Chin. J. Anal. Chem.(分析化学), 2012, 40(11):1680-1685 (in Chinese).

Huang B X, Kim H Y. Probing three-dimensional structure of bovine serum albumin by chemical cross-linking and mass spectrometry [J]. J. Am. Soc. Mass Spectrom., 2004, 15(8):1237-1247.

Zhang Y F, Wang J Z, Wang C Y, et al. Determination of ceftriaxone with fluorescence method [J]. Herald Med.(医药导报), 2007, 26(3):296-297 (in Chinese).

Li C S. Adverse reactions ceftriaxone [J]. J. Physiol. Sci.(四川生理科学杂志), 2009, 31(2):85-87 (in Chinese).

Woodfield J C, Van Rij A M, Pettigrew R A, et al. A comparison of the prophylactic efficacy of ceftriaxone and cefotaxime in abdominal surgery [J]. Am. Surg., 2003, 185(1):45-49.

Liu Y, Yang S X. Determination of ceftriaxone sodium/tazobactam sodium for injection by HPLC [J]. Chin. Pharm. J.(中国药学杂志), 2005, 40(20):1584-1586 (in Chinese).

Tu L, Hu C Q. Ceftriaxone capillary electrophoresis assay and related substances content [J]. Chin. Pharm. Anal.(药物分析杂志), 2005, 25(3):303-307 (in Chinese).

Liu B, Wang J Z, Man Y. Ceftriaxone flow injection chemiluminescence inhibition assay [J]. J. Instrum. Anal.(分析测试学报), 2003, 22(4):45-47 (in Chinese).

Shi Z Z, Wang F L, Wang J Z. Determination of drug content in ceftriaxone single sweep polarography [J]. Phys.Testing Chem. Anal., Part B: Chem. Anal.(理化检验(化学分册) ), 2006, 42(5):329-333 (in Chinese).

Costa-Fernndez J M, Pereiro R, Sanz-Medel A. The use of luminescent quantum dots for optical sensing [J]. Anal. Chem., 2006, 25(3):207-218.

Miao Y M, Zhang Z F, Gong Y, et al. Self-assembly of manganese doped zinc sulfide quantum dots/CTAB nanohybrids for detection of rutin [J]. Biosens. Bioelectron., 2014, 52:271-276.

He Y, Wang H F, Yan X P. Self-assembly of Mn-doped ZnS quantum dots/octa (3-aminopropyl) octasilsequioxane octahydrochloride nanohybrids for optosensing DNA [J]. Chem.Eur. J., 2009, 15(22):5436-5440.

Zhang A M, Yan W, Wang H S. Interactions of proteins with CdTe/CdS quantum dots ceftriaxone [J]. Chin. J. Anal. Chem.(分析化学), 2014, 36(4):444-448 (in Chinese).

Wang G, Wang D, Li X, et al. Exploring the binding mechanism of dihydropyrimidinones tohuman serum albumin: Spectroscopic and molecular modeling techniques [J]. Colloids Surf. B: Biointerf., 2011, 84(1):272-279.

Shen X C, Liang H, He X W. Circular dichroism spectroscopy and protein conformation of progress [J]. Chin. J. Anal. Chem.(分析化学), 2004, 32(3):388-394 (in English).

浏览量

下载量

CSCD

文章被引用时，请邮件提醒。

提交

工具集

关联资源

金属卤化物CH₃NH₃PbBr₃钙钛矿量子点快速检测新烟碱类农药

磷化铟量子点及其电致发光研究现状和挑战

基于量子点@有序介孔复合材料的Micro⁃LED色转换特性

PbS量子点掺杂聚合物宽带光纤放大器

CsPb_xSn_1-_xBr₃/a⁃ZrP复合材料的光学性能及其在白色发光二极管中的应用