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CdSe量子点“开关”的构建及其在药物分析中的应用
Construction of CdSe Quantum Dots “Switch” and Its Application in Pharmaceutical Analysis
- 发光学报 2015年36卷第3期 页码：312-316
- 作者机构：
1. 广西大学化学化工学院 广西石化资源加工及过程强化技术重点实验室,广西 南宁,530004
2. 广西大学 材料科学与工程学院,广西 南宁,530004
- 作者简介：
- 基金信息：广西壮族自治区教育厅科研基金(2013YB014)();广西石化资源加工及过程强化技术重点实验室开放基金(2013K007)资助项目()
DOI：10.3788/fgxb20153603.0312
中图分类号： O482.31纸质出版日期：2015-3-3，
网络出版日期：2015-1-12，
收稿日期：2014-11-27，
修回日期：2015-1-6，
扫 描 看 全 文
杨昆, 陈林情, 赵慧凯等. CdSe量子点“开关”的构建及其在药物分析中的应用[J]. 发光学报, 2015,36(3): 312-316
YANG Kun, CHEN Lin-qing, ZHAO Hui-kai etc. Construction of CdSe Quantum Dots “Switch” and Its Application in Pharmaceutical Analysis[J]. Chinese Journal of Luminescence, 2015,36(3): 312-316
杨昆, 陈林情, 赵慧凯等. CdSe量子点“开关”的构建及其在药物分析中的应用[J]. 发光学报, 2015,36(3): 312-316 DOI： 10.3788/fgxb20153603.0312.
YANG Kun, CHEN Lin-qing, ZHAO Hui-kai etc. Construction of CdSe Quantum Dots “Switch” and Its Application in Pharmaceutical Analysis[J]. Chinese Journal of Luminescence, 2015,36(3): 312-316 DOI： 10.3788/fgxb20153603.0312.
摘要
以CdCl
2
2.5H
2
O
Na
2
SeO
3
和NaBH
4
为反应物
制备巯基丁二酸稳定的CdSe量子点。在合成的量子点中加入一定浓度的铜离子
铜离子结合于量子点表面而使其荧光猝灭。在猝灭后的体系中加入青霉胺
由于青霉胺能使结合于量子点表面的铜离子释放出来而使量子点的荧光恢复。据此
建立了一种CdSe量子点"开关"测定青霉胺的新方法。在选定的实验条件下
方法的线性范围为1~72 g/mL
检出限为0.031 g/mL。应用于实际样品中青霉胺的测定
结果令人满意。
Abstract
CdSe quantum dots were prepared by the reaction of CdCl
2
2.5H
2
O
Na
2
SeO
3
and NaBH
4
in water and in the presence of mercaptosuccinic acid as stabilizer. The fluorescence of quantum dots can be significantly quenched by copper ions. However
in the presence of penicillamine
copper ions were released from the quantum dots and thus restored the fluorescence of quantum dots. A new type of fluorescence turn-on assay for detection of penicillamine using quantum dots/copper ion ensemble is developed. Under the selected conditions
the response was linearly proportional to the concentration of penicillamine from 1 to 72 g/mL
the limit of detection was 0.031 g/mL. The developed method has been successfully applied to the detection of penicillamine in real samples with satisfactory results.
关键词
量子点铜离子青霉胺荧光测定
Keywords
quantum dotscopper ionspenicillaminefluorescencedetermination
references
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