偏最小二乘法结合流动注射化学发光法同时测定芦丁和维生素C

曾华金; 梁会丽; 游静; 李世君; 睢国慧; 屈凌波

doi:10.3788/fgxb20133403.0369

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偏最小二乘法结合流动注射化学发光法同时测定芦丁和维生素C

半导体发光、激光和光电性质 | 更新时间：2020-08-12

- 偏最小二乘法结合流动注射化学发光法同时测定芦丁和维生素C
- Simultaneous Determination of Ascorbic Acid and Rutin by Flow-injection Chemiluminescence Method Using Partial Least Squares Regression
- 发光学报 2013年34卷第3期页码：369-374
- 作者机构：
  
  1. 郑州大学药学院, 河南郑州 450001
  2. 郑州大学化学分子工程学院, 河南郑州 450001
  3. 河南工业大学化学化工学院, 河南郑州 450001
- 作者简介：
- 基金信息：
- DOI：10.3788/fgxb20133403.0369
  中图分类号： O657.3
- 纸质出版日期：2013-3-10，
  
  收稿日期：2012-11-30，
  
  修回日期：2012-12-13，
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曾华金, 梁会丽, 游静, 李世君, 睢国慧, 屈凌波. 偏最小二乘法结合流动注射化学发光法同时测定芦丁和维生素C[J]. 发光学报, 2013,34(3): 369-374

ZENG Hua-jin, LIANG Hui-li, YOU Jing, LI Shi-jun, SUI Guo-hui, QU Ling-bo. Simultaneous Determination of Ascorbic Acid and Rutin by Flow-injection Chemiluminescence Method Using Partial Least Squares Regression[J]. Chinese Journal of Luminescence, 2013,34(3): 369-374
曾华金, 梁会丽, 游静, 李世君, 睢国慧, 屈凌波. 偏最小二乘法结合流动注射化学发光法同时测定芦丁和维生素C[J]. 发光学报, 2013,34(3): 369-374 DOI： 10.3788/fgxb20133403.0369.

ZENG Hua-jin, LIANG Hui-li, YOU Jing, LI Shi-jun, SUI Guo-hui, QU Ling-bo. Simultaneous Determination of Ascorbic Acid and Rutin by Flow-injection Chemiluminescence Method Using Partial Least Squares Regression[J]. Chinese Journal of Luminescence, 2013,34(3): 369-374 DOI： 10.3788/fgxb20133403.0369.

摘要

在碱性条件下

芦丁和维生素C对鲁米诺-铁氰化钾化学发光体系具有显著的抑制作用

但两者峰值出现的时间存在明显差异。基于这一特点

本文将偏最小二乘法与流动注射化学发光法结合

建立了偏最小二乘法-流动注射化学发光同时检测芦丁和维生素C的新方法。在优化实验条件下

发光强度

与芦丁浓度和维生素C质量浓度在0.02～0.6 mgL

-1

和0.1～20 mgL

-1

范围内呈线性关系

检出限分别为4.5 gmL

-1

和8.9 ngmL

-1

(信噪比为3)。经实际测定复方芦丁片和人体尿液中芦丁和维生素C的含量表明

该方法具有灵敏度高、分析速度快及操作简便等优点

适合用于药品及生物样品等复杂样品中芦丁和维生素C的测定。

Abstract

A flow-injection chemiluminescence (CL) system was developed for the simultaneous determination of ascorbic acid (AA) and rutin (RT) using partial least squares (PLS) calibration. This method is based on that fact that both AA and RT can quantitatively reduce Fe

to Fe

and that the reaction rates of AA and RT with Fe

are different. The calibration curve was linear over the concentration range of 0.1～20.0 mgL

-1

and 0.02～0.6 mgL

-1

for AA and RT

respectively. The limits of detection (LOD) for AA and RT were 8.9 gL

-1

and 4.5 gL

-1

respectively

which was calculated according to the International Union of Pure and Applied Chemistry (IUPAC) definition that is 3 times the standard deviation of the blank value. The proposed method was successfully applied to the simultaneous determination of both analytes in pharmaceutical formulations and human urine samples. The results indicate that the method offers the potential advantages of high sensitivity

simplicity and rapidity for AA and RT determination in pharmacy and biological samples.

关键词

偏最小二乘法流动注射化学发光芦丁维生素C

Keywords

flow-injection chemiluminescence (FI-CL)partial least squares (PLS)rutinascorbic acid

references

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