头孢匹胺钠与牛血清白蛋白的相互作用机理及共存金属离子的影响

刘保生; 闫潇娜; 曹世娜; 种宝红; 吕运开

doi:10.3788/fgxb20123309.1018

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头孢匹胺钠与牛血清白蛋白的相互作用机理及共存金属离子的影响

光谱分析 | 更新时间：2020-08-12

- 头孢匹胺钠与牛血清白蛋白的相互作用机理及共存金属离子的影响
- Interaction of Cefpiramide Sodium with Bovine Serum Albumin and The Effect of Coexistent Metal Ion on The Reaction
- 发光学报 2012年33卷第9期页码：1018-1024
- 作者机构：
  
  河北大学化学与环境科学学院药物化学与分子诊断教育部重点实验室,河北保定,071002
- 作者简介：
- 基金信息：
  
  国家自然科学基金(20675024)();河北省重点基础研究项目(10967126D)资助项目()
- DOI：10.3788/fgxb20123309.1018
  中图分类号： O657.3
- 纸质出版日期：2012-9-10，
  
  收稿日期：2012-3-26，
  
  修回日期：2012-6-28，
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刘保生, 闫潇娜, 曹世娜, 种宝红, 吕运开. 头孢匹胺钠与牛血清白蛋白的相互作用机理及共存金属离子的影响[J]. 发光学报, 2012,33(9): 1018-1024

LIU Bao-sheng, YAN Xiao-na, CAO Shi-na, CHONG Bao-hong, LV Yun-kai. Interaction of Cefpiramide Sodium with Bovine Serum Albumin and The Effect of Coexistent Metal Ion on The Reaction[J]. Chinese Journal of Luminescence, 2012,33(9): 1018-1024
刘保生, 闫潇娜, 曹世娜, 种宝红, 吕运开. 头孢匹胺钠与牛血清白蛋白的相互作用机理及共存金属离子的影响[J]. 发光学报, 2012,33(9): 1018-1024 DOI： 10.3788/fgxb20123309.1018.

LIU Bao-sheng, YAN Xiao-na, CAO Shi-na, CHONG Bao-hong, LV Yun-kai. Interaction of Cefpiramide Sodium with Bovine Serum Albumin and The Effect of Coexistent Metal Ion on The Reaction[J]. Chinese Journal of Luminescence, 2012,33(9): 1018-1024 DOI： 10.3788/fgxb20123309.1018.

摘要

研究了不同温度下牛血清白蛋白(BSA)与头孢匹胺钠(CPMS)的相互作用及常见金属离子(Mg

、Zn

、Cu

、Fe

、Co

、Ni

)对BSA-CPMS体系的影响。结果表明:CPMS能使BSA的荧光发生猝灭

其过程为静态猝灭并伴有非辐射能量转移;CPMS通过静电引力与BSA结合

结合常数为10

数量级

结合位点数约为1;结合主要作用于BSA的亚结构ⅡA中

结合距离

≈2.60 nm。同步荧光光谱和圆二色谱(CD)均表明CPMS使BSA构象发生了变化。

Abstract

The interaction between bovine serum albumin (BSA) and cefpiramide sodium (CPMS) was investigated at different temperatures. The effect of common metal ions (Mg

) on the BSA-CPMS system was also researched. The results show that CPMS can quench the intrinsic fluorescence of BSA significantly

and the quenching mechanism is a static quenching process which follows the Förester spectroscopy energy transfer. The electrostatic force played an important role on the conjugation reaction between BSA and CPMS. The binding constants (

) was in the order of 10

and the number of binding site (

) in the binary system was approximately equal to 1. The binding distance (

) was about 2.60 nm and the primary binding for CPMS was located at sub-domainⅡA of BSA. Synchronous spectra and Circular dichroism(CD) spectra revealed that the microenvironment and the conformation of BSA were changed during the conjugation reaction.

关键词

牛血清白蛋白头孢匹胺钠金属离子相互作用

Keywords

bovine serum albumincefpiramide sodiummetal ionsinteraction

references

Sun Q, Shi M, Shao W, et al. Tissue distribution and pulmonary targeting studies of cefpiramide sodium-loaded liposomes [J]. J. Drug Target., 2011, 19(1):49-55.

Shaikh S M T, Seetharamappa J, Ashoka S, et al. A study of the interaction between bromopyrogallol red and bovine serum albumin by spectroscopic methods [J]. Dyes and Pigments, 2007, 73(2):211-216.

Yu Y M, Feng J C, Liu Y. Spectroscopic Study of the interaction between sulfamethoxazole and bovine serum albumin in the presence of Fe3+ [J]. Acta Chimica Sinica (化学学报), 2011, 69(2):190-198 (in Chinese).

Steiner R F, Weinryb L. Exicited States of Protein and Nucleic Acid [M]. New York: Plenum Press, 1971:40.

Zhang Z H, Zang S L, Geng B, et al. Study on interaction of butyltin compounds with BSA by UV and CD spectra [J]. J. Anal. Sci.(分析科学学报), 2005, 21(2):179-181 (in Chinese).

Liu J Q, Tian J N, Zhang J Y, et al. Interaction of magnolol with bovine serum albumin:A fluorescence- quenching study [J]. Anal. Bioanal. Chem., 2003, 376(6):864-867.

Bi S Y, Ding L, Song D Q, et al. Studies on the binding mechanism of several antibiotics and human serum albumin [J]. Acta Chimica Sinica (化学学报), 2005, 63(23):2169-2173 (in Chinese).

Sulkowska A, Maciazek-Jurczyk M, Bojko B, et al. Competitive binding of phenylbutazone and colchicine to serum albumin in multidrug therapy [J]. J. Mol. Struct., 2008, 881(1-3):97-106.

Pei M Y, Zheng X F, Cao H Y, et al. Spectroscopic studies on interaction between 3-bromopyruvic acid and human serum albumin [J]. Chin. J. Anal. Chem. (分析化学), 2010, 38(7):948-952 (in Chinese).

Liu B S, Yang C, Wang J, et al. Luminescence mechanism study of the conjugation reaction between cefpirome sulfate and bovine serum albumin [J]. Chin. J. Lumin.(发光学报), 2011, 32(3):293-299 (in Chinese).

Chen D W, Xie Q J, Su Y H, et al. Fluorescent studies on the interactions between Au nanoparticles and quercetin as well as bovine hemoglobin [J]. J. Anal. Sci.(分析科学学报), 2008, 24(3):259-264 (in Chinese).

Mandal P, Bardhan M, Ganguly T. A detailed spectroscopic study on the interaction of rhodamine 6G with human hemoglobin [J]. J. Photochem. Photobiol. B, 2010, 99(2):78-86.

Zhang Q, Chen X, Bai X H. Simultaneous and fast research of protein binding characteristic of six flavonoids drugs by hollow fiber liquid phase microextraction [J]. Chin. J. Anal. Chem.(分析化学), 2011, 39(6):857-862 (in Chinese).

Aodeng G W, Jin Y C. Spectroscopic studies on the interaction between fenbufen and bovine serum albumin [J]. Chin. J. Lumin.(发光学报), 2011, 32(4):404-410 (in Chinese).

Shao S, Qiu J. Effecting of metal ions on the interaction between zidovudine and bovine serum albumin [J]. Acta Phys. Chim. Sin. (物理化学学报), 2009, 25(7):1342-1346 (in Chinese).

Fei Y, Lu G C, Fan G R, et al. Studies on interaction of sinafloxacin with bovine serum albumin and effect of the coexistent metal ions on the reaction [J]. Spectrosc. Spectral. Anal. (光谱学与光谱分析), 2008, 28(11):2609-2614 (in Chinese).

Wu G H, Wang J, Chen J L, et al. Bovine serum albumin in the presence of zinc(Ⅱ) by fluorescence method [J]. Spectrosc. Spectral. Anal.(光谱学与光谱分析), 2008, 28(4):913-916 (in Chinese).

Wang C X, Ye L, Yan F F, et al. Spectroscopic studies on the interaction between rifabutin andhuman serum albumin [J]. Chem. J. Chinese Universities (高等学校化学学报), 2007, 28(12):2280-2283 (in Chinese).

Wang Y, Chen Y P, Zhang Y Z. Investigation on the interaction of 2,4-dichlorophenol with human serum albumin [J]. Chem. Bioengin.(化学与生物工程), 2011, 28(5):28-33 (in Chinese).

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