CdTe量子点-罗丹明B荧光共振能量转移法测定溶菌酶

高桂园; 刘璐; 付璇; 杨冉; 屈凌波

doi:10.3788/fgxb20123308.0911

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CdTe量子点-罗丹明B荧光共振能量转移法测定溶菌酶

光谱分析 | 更新时间：2020-08-12

- CdTe量子点-罗丹明B荧光共振能量转移法测定溶菌酶
- Fluorescence Resonance Energy Transfer Between CdTe QDs and Rhodamine B with Its Application in The Determination of Lysozyme
- 发光学报 2012年33卷第8期页码：911-915
- 作者机构：
  
  1. 郑州大学化学与分子工程学院, 河南郑州 450001
  2. 河南工业大学化学化工学院, 河南郑州 450001
- 作者简介：
- 基金信息：
- DOI：10.3788/fgxb20123308.0911
  中图分类号： O482.31
- 纸质出版日期：2012-8-10，
  
  收稿日期：2012-4-20，
  
  修回日期：2012-5-23，
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高桂园, 刘璐, 付璇, 杨冉, 屈凌波. CdTe量子点-罗丹明B荧光共振能量转移法测定溶菌酶[J]. 发光学报, 2012,33(8): 911-915

GAO Gui-yuan, LIU Lu, FU Xuan, YANG Ran, QU Ling-bo. Fluorescence Resonance Energy Transfer Between CdTe QDs and Rhodamine B with Its Application in The Determination of Lysozyme[J]. Chinese Journal of Luminescence, 2012,33(8): 911-915
高桂园, 刘璐, 付璇, 杨冉, 屈凌波. CdTe量子点-罗丹明B荧光共振能量转移法测定溶菌酶[J]. 发光学报, 2012,33(8): 911-915 DOI： 10.3788/fgxb20123308.0911.

GAO Gui-yuan, LIU Lu, FU Xuan, YANG Ran, QU Ling-bo. Fluorescence Resonance Energy Transfer Between CdTe QDs and Rhodamine B with Its Application in The Determination of Lysozyme[J]. Chinese Journal of Luminescence, 2012,33(8): 911-915 DOI： 10.3788/fgxb20123308.0911.

摘要

合成了以硫代乙醇酸为稳定剂的CdTe量子点

以发射波长为530 nm的量子点为供体

罗丹明B为受体

建立一种以十六烷基三甲基溴化铵为介质的荧光共振能量体系检测溶菌酶含片中溶菌酶含量的方法。结果表明:在pH=5.0时

溶菌酶的浓度与共振能量转移效率降低值在2×10

-7

~ 8×10

-6

mol·L

-1

范围内呈线性关系

其线性方程为

=306.07-13.85

Abstract

The water-soluble CdTe was synthesized using thioglycolic acid as stabilizer. The fluorescence resonance energy transfer (FRET) between the CdTe QDs and Rhodamine B (RhB) in the presence of cetyltrimethylammonium bromide (CTAB) was characterized

in which the QDs with an emission wavelength of 530 nm was used as energy donor and rhodamine B as acceptor. Lysozyme content in lysozyme buccal tablets was determined by fluorescence resonance energy transfer. The results showed that the extent of fluorescence energy transfer quenching was linearly proportional to the concentration of lysozyme from 2?10

-7

mol稬

-1

to 8?10

-6

mol稬

-1

with a correlation coefficient of 0.991 0. The minimum detection concentration was 2?10

-8

mol稬

-1

RSD and the average recovery was 5.8% and 101%(

=5)

respectively.

关键词

CdTe罗丹明B荧光共振能量转移溶菌酶

Keywords

CdTeRhBFRETlysozyme

references

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Dragoni I, Balzaretti C, Rossini S, et al. Detection of hen lysozyme on proteic profiles of grana padano cheese through SELDI-TOF MS high-throughput technology during the ripening process [J].Food Analytical Methods, 2011, 4(1):233-239.

Hemmen E, Mahana W, Jollds P, et al. Common antigenic properties of a g-type (goose) and a c-type (duck) egg white lysozyme: Antibody responses in rabbits and mice [J]. Experientia, 1992, 48(1):79-84.

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Ge S G, Lu J J, Yan M, et al. Fluorescence resonance energy transfer sensor between quantum dot donors and neutral red acceptors and its detection of BSA in micelles [J]. Dyes and Pigments, 2011, 91(3):304-308.

Tao H L, Li S H, Li J P. Fluorescence resonance energy transfer between quantum dots of CdSe and CdTe and its application for determination of serum prostate-specific antigen [J].Chinese Journal of Analytical Chemistry (分析化学), 2012, 40(2):224-229 (in Chinese).

Bhuvaneswari J, Fathima A K, Rajagopal S. Rhenium (I)-based fluorescence resonance energy transfer probe for conformational changes of bovine serum albumin [J]. Journal of Photochemistry and Photobiology A: Chemistry, 2012, 227(1):38-44.

Hu B, Zhang L P, Chen M L, et al. The inhibition of fluorescence resonance energy transfer between quantum dots for glucose assay [J]. Biosensors and Bioelectronics, 2012, 32(1):82-88.

Liu H L, Wang Y H, Shen A G, et al. Highly selective and sensitive method for cysteine detection based on fluorescence resonance energy transfer between FAM-tagged ssDNA and graphene oxide [J]. Talanta, 2012, 93(15):330-335.

Yu W W, Qu L H, Guo W Z, et al. Experimental determination of the extinction coefficient of CdTe, CdSe, and CdS nanocrystals [J]. Chem.Mater, 2003, 15(14):2854-2860.

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