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1. 郑州大学 化学与分子工程学院, 河南 郑州 450001
2. 河南工业大学 化学化工学院, 河南 郑州 450001
纸质出版日期:2012-8-10,
收稿日期:2012-4-20,
修回日期:2012-5-23,
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高桂园, 刘璐, 付璇, 杨冉, 屈凌波. CdTe量子点-罗丹明B荧光共振能量转移法测定溶菌酶[J]. 发光学报, 2012,33(8): 911-915
GAO Gui-yuan, LIU Lu, FU Xuan, YANG Ran, QU Ling-bo. Fluorescence Resonance Energy Transfer Between CdTe QDs and Rhodamine B with Its Application in The Determination of Lysozyme[J]. Chinese Journal of Luminescence, 2012,33(8): 911-915
高桂园, 刘璐, 付璇, 杨冉, 屈凌波. CdTe量子点-罗丹明B荧光共振能量转移法测定溶菌酶[J]. 发光学报, 2012,33(8): 911-915 DOI: 10.3788/fgxb20123308.0911.
GAO Gui-yuan, LIU Lu, FU Xuan, YANG Ran, QU Ling-bo. Fluorescence Resonance Energy Transfer Between CdTe QDs and Rhodamine B with Its Application in The Determination of Lysozyme[J]. Chinese Journal of Luminescence, 2012,33(8): 911-915 DOI: 10.3788/fgxb20123308.0911.
合成了以硫代乙醇酸为稳定剂的CdTe量子点
以发射波长为530 nm的量子点为供体
罗丹明B为受体
建立一种以十六烷基三甲基溴化铵为介质的荧光共振能量体系检测溶菌酶含片中溶菌酶含量的方法。结果表明:在pH=5.0时
溶菌酶的浓度与共振能量转移效率降低值在2×10
-7
~ 8×10
-6
mol·L
-1
范围内呈线性关系
其线性方程为
Y
=306.07-13.85
X
相关系数为0.991 0
检出限为2×10
-8
mol·L
-1
RSD为5.8%
平均回收率为101%(
n
=5)。
The water-soluble CdTe was synthesized using thioglycolic acid as stabilizer. The fluorescence resonance energy transfer (FRET) between the CdTe QDs and Rhodamine B (RhB) in the presence of cetyltrimethylammonium bromide (CTAB) was characterized
in which the QDs with an emission wavelength of 530 nm was used as energy donor and rhodamine B as acceptor. Lysozyme content in lysozyme buccal tablets was determined by fluorescence resonance energy transfer. The results showed that the extent of fluorescence energy transfer quenching was linearly proportional to the concentration of lysozyme from 2?10
-7
mol稬
-1
to 8?10
-6
mol稬
-1
with a correlation coefficient of 0.991 0. The minimum detection concentration was 2?10
-8
mol稬
-1
RSD and the average recovery was 5.8% and 101%(
n
=5)
respectively.
CdTe罗丹明B荧光共振能量转移溶菌酶
CdTeRhBFRETlysozyme
Wang T X, Liu Z H, Xia L Y, et al. Determination of lysozyme in honey by ultraviolet spectrophotometry [J]. Food Science and Technology (食品科技), 2010, 35(4):292-297 (in Chinese).
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Dragoni I, Balzaretti C, Rossini S, et al. Detection of hen lysozyme on proteic profiles of grana padano cheese through SELDI-TOF MS high-throughput technology during the ripening process [J].Food Analytical Methods, 2011, 4(1):233-239.
Hemmen E, Mahana W, Jollds P, et al. Common antigenic properties of a g-type (goose) and a c-type (duck) egg white lysozyme: Antibody responses in rabbits and mice [J]. Experientia, 1992, 48(1):79-84.
Li J, Mei F, Li W Y, et al. Study on the fluorescence resonance energy transfer between CdTe QDs and butyl-rhodamine B in the presence of CTMAB and its application on the detection of Hg(Ⅱ) [J]. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 2007, 70(4):811-817.
Liu Y L, Lv X, Zhao Y, et al. A naphthalimide-rhodamine ratiometric fluorescent probe for Hg2+ based on fluorescence resonance energy transfer [J]. Dyes and Pigments, 2012, 92(3):909-915.
Ge S G, Lu J J, Yan M, et al. Fluorescence resonance energy transfer sensor between quantum dot donors and neutral red acceptors and its detection of BSA in micelles [J]. Dyes and Pigments, 2011, 91(3):304-308.
Tao H L, Li S H, Li J P. Fluorescence resonance energy transfer between quantum dots of CdSe and CdTe and its application for determination of serum prostate-specific antigen [J].Chinese Journal of Analytical Chemistry (分析化学), 2012, 40(2):224-229 (in Chinese).
Bhuvaneswari J, Fathima A K, Rajagopal S. Rhenium (I)-based fluorescence resonance energy transfer probe for conformational changes of bovine serum albumin [J]. Journal of Photochemistry and Photobiology A: Chemistry, 2012, 227(1):38-44.
Hu B, Zhang L P, Chen M L, et al. The inhibition of fluorescence resonance energy transfer between quantum dots for glucose assay [J]. Biosensors and Bioelectronics, 2012, 32(1):82-88.
Liu H L, Wang Y H, Shen A G, et al. Highly selective and sensitive method for cysteine detection based on fluorescence resonance energy transfer between FAM-tagged ssDNA and graphene oxide [J]. Talanta, 2012, 93(15):330-335.
Yu W W, Qu L H, Guo W Z, et al. Experimental determination of the extinction coefficient of CdTe, CdSe, and CdS nanocrystals [J]. Chem.Mater, 2003, 15(14):2854-2860.
Zheng J J, Zheng Z H. An inerratic variation of nonlinear co-efficient with temperature in CdSe/ZnSe self assembled quantum dots [J]. Chin. J. Lumin.(发光学报), 2010, 31(6):836-841 (in English) .
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