丁二酰化壳寡糖铕配合物的合成及与牛血清白蛋白的相互作用

李小芳; 冯小强; 杨声; 张玉霞

doi:10.3788/fgxb20123308.0905

您当前的位置：

首页 >

文章列表页 >

丁二酰化壳寡糖铕配合物的合成及与牛血清白蛋白的相互作用

光谱分析 | 更新时间：2020-08-12

- 丁二酰化壳寡糖铕配合物的合成及与牛血清白蛋白的相互作用
- Synthesis and Interaction of Succinic-oligochitosan-Eu(Ⅲ) Complex with Bovine Serum Albumin
- 发光学报 2012年33卷第8期页码：905-910
- 作者机构：
  
  1. 天水师范学院生命科学与化学学院,甘肃天水,741001
  2. 定西师范高等专科学校,甘肃定西,743000
- 作者简介：
- 基金信息：
  
  天水师范学院物理无机化学重点学科基金(ZD0840)();天水师范学院"青蓝"人才工程和天水师范学院中青年教师科研项目(TSA1003)资助项目()
- DOI：10.3788/fgxb20123308.0905
  中图分类号： O614.338
- 纸质出版日期：2012-8-10，
  
  收稿日期：2012-5-7，
  
  修回日期：2012-6-3，
扫描看全文
李小芳, 冯小强, 杨声, 张玉霞. 丁二酰化壳寡糖铕配合物的合成及与牛血清白蛋白的相互作用[J]. 发光学报, 2012,33(8): 905-910

LI Xiao-fang, FENG Xiao-qiang, YANG Sheng, ZHANG Yu-xia. Synthesis and Interaction of Succinic-oligochitosan-Eu(Ⅲ) Complex with Bovine Serum Albumin[J]. Chinese Journal of Luminescence, 2012,33(8): 905-910
李小芳, 冯小强, 杨声, 张玉霞. 丁二酰化壳寡糖铕配合物的合成及与牛血清白蛋白的相互作用[J]. 发光学报, 2012,33(8): 905-910 DOI： 10.3788/fgxb20123308.0905.

LI Xiao-fang, FENG Xiao-qiang, YANG Sheng, ZHANG Yu-xia. Synthesis and Interaction of Succinic-oligochitosan-Eu(Ⅲ) Complex with Bovine Serum Albumin[J]. Chinese Journal of Luminescence, 2012,33(8): 905-910 DOI： 10.3788/fgxb20123308.0905.

摘要

合成并表征了丁二酰化壳寡糖铕配合物

在模拟人体生理条件下

运用紫外和荧光光谱研究了配合物与牛血清白蛋白(BSA)的相互作用。结果表明:随着配合物浓度的增加

BSA的紫外光谱表现出增色效应并伴有蓝移;配合物与BSA形成复合物

从而猝灭BSA内源荧光

该猝灭机制为静态猝灭。计算得到室温下配合物与BSA的结合常数和结合位点数分别为2.068 2×10

L·mol

-1

和1.094 72。

Abstract

Succinic-oligochitosan-Eu(Ⅲ) complex was synthesized and characterized. Under simulated physiological conditions

the interaction between complex and bovine serum albumins (BSA) was studied by ultraviolet spectrum and fluorescence spectrum. The results showed that the absorption intensity of BSA increased with the concentration of complex. The complex quenched the fluorescence of BSA and it was static quenching

and then the binding constant and binding sites were calculated . They were 2.068 2×10

L·mol

-1

and 1.094 72 at room temperature

respectively.

关键词

丁二酰化壳寡糖铕牛血清白蛋白相互作用

Keywords

succinic-oligochitosan-Eu(Ⅲ)bovine serum albumininteraction

references

Sugio S, Kashima A, Mochizuki S, et al. Crystal structure of human serum albumin at 2.5 nm resolution [J]. Protein Eng., 1999, 12(6):439-446.

Wang Z, Zheng L, Yang S, et al. N-acetyl chitooligosaccharide is a potent angiogenic inhibitor both in vivo and in vitro [J]. Biochem. Biophys. Res. Commun., 2007, 357(1):26-31.

Liu B S, Xue C L, Wang J, et al. Studies on the interaction between ciprofloxacin and ofloxacin in presence of bovine serum albumin by spectroscopic methods [J]. Chin. J. Lumin. (发光学报), 2010, 31(2):285-290 (in Chinese).

Deng R W, Zhang Z S. Rare earth medicine and pharmacy property [J]. Chin. Rare Earth (稀土), 1987, 8(3):36-47 ( in Chinese).

Ren Q X, Zhai Y C, Bai Y H, et al. Synthesis, coordination mechanism and anti-hydroxyl free radical activity of coordination compounds of chitooligosaccharide with PrCl3 and DyCl3 [J]. Chin. Rare Earth (稀土), 2009, 30(3):57-61 (in Chinese).

Wang K J, Wen H R, Liu F, et al. Syntheses and spectral characterization of low-molecular-weight chitosan-Ce(Ⅲ) complexes [J]. Spectroscopy and Spectral Analysis (光谱学与光谱分析), 2006, 26(7):1277-1280 (in Chinese).

Yang Z F, He Q Z, Xu D F, et al. Preparation, characterization and antibacterial properties of chitosan-RE(Ⅲ) complexes [J]. Chin. Rare Earth (稀土), 2009, 30(6):50-54 (in Chinese).

Li X F, Feng X Q, Yang S, et al. Synthesis, antibacterial activity and interaction with bovine serum albumin of chitosan-rare earth complexes [J]. Journal of Functional Polymers (功能高分子学报), 2010, 23(2):197-201 (in Chinese).

Feng X Q, Li X F, Yang S, et al. Study on the synthesis of rare earth complexes of o-carboxymethyl chitosan and the IR interaction with bovine serum albumin [J]. Chinese Journal of Analysis Laboratory (分析试验室), 2010, 29(11):5-8 (in Chinese).

Feng X Q, Fu G Q, Li X F, et al. Interaction of rare earth with bovine serum albumin [J]. Chin. J. Lumin.(发光学报), 2011, 32(2):205-209 (in Chinese).

Xie M X, Xu X Y, Wang Y D. Spectroscopic investigation of the interaction between 2,3-dihydro-4',5,7-trihydroxyflavone and human serum albumin [J]. Acta Chimica Sinica (化学学报), 2005, 63(22):2055-2062 (in Chinese).

Chen G Z. Fluorescence Analytical Method [M]. Beijing: Science Press, 1990:122-189 (in Chinese).

Zhang B L, Wang W Q, Yuan R Y, et al. Spectroscopic studies on binding of anthraquinones and flavonoids to bovine serum albumin [J]. Acta Chimica Sinica (化学学报), 1994, 52(12):1213-1217 (in Chinese).

Zhang X W, Zhao F L, Li K A, et al. Studies on the reaction between ciprofloxacin and bovine serum albumin [J]. Chemical Journal of Chinese Universities (高等学校化学学报), 1999, 20(7):1063-1067 (in Chinese).

浏览量

下载量

CSCD

文章被引用时，请邮件提醒。

提交

工具集

关联资源

光谱法及分子对接技术研究黄体酮与牛血清白蛋白的结合机制

改进荧光法研究氟洛芬与牛血清白蛋白的相互作用

头孢匹胺钠与牛血清白蛋白的相互作用机理及共存金属离子的影响

芬布芬与牛血清白蛋白相互作用的荧光光谱检测

环丙沙星、氧氟沙星同时与牛血清白蛋白分子作用的荧光光谱