荜茇宁与邻硝基苯基荧光酮的荷移反应及其测定

敖登高娃; 安娜; 领小; 苗澍

doi:10.3788/fgxb20123305.0558

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荜茇宁与邻硝基苯基荧光酮的荷移反应及其测定

光谱分析 | 更新时间：2020-08-12

- 荜茇宁与邻硝基苯基荧光酮的荷移反应及其测定
- Spectrophotometric Determination of Piperlonguminine Based on The Charge Transfer Reaction Between Piperlonguminine and o-Nitrophenyl Fluorone
- 发光学报 2012年33卷第5期页码：558-561
- 作者机构：
  
  内蒙古大学化学化工学院, 内蒙古呼和浩特 010021
- 作者简介：
- 基金信息：
  
  国家自然科学基金(20965004)();内蒙古自然科学基金(2010BS1203)资助项目()
- DOI：10.3788/fgxb20123305.0558
  中图分类号： O657.32
- 纸质出版日期：2012-5-10，
  
  网络出版日期：2012-5-10，
  
  收稿日期：2011-12-1，
  
  修回日期：2012-3-16，
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敖登高娃, 安娜, 领小, 苗澍. 荜茇宁与邻硝基苯基荧光酮的荷移反应及其测定[J]. 发光学报, 2012,(5): 558-561

AODENG Gaowa, AN Na, LING Xiao, MIAO Shu. Spectrophotometric Determination of Piperlonguminine Based on The Charge Transfer Reaction Between Piperlonguminine and <em>o</em>-Nitrophenyl Fluorone[J]. Chinese Journal of Luminescence, 2012,(5): 558-561
敖登高娃, 安娜, 领小, 苗澍. 荜茇宁与邻硝基苯基荧光酮的荷移反应及其测定[J]. 发光学报, 2012,(5): 558-561 DOI： 10.3788/fgxb20123305.0558.

AODENG Gaowa, AN Na, LING Xiao, MIAO Shu. Spectrophotometric Determination of Piperlonguminine Based on The Charge Transfer Reaction Between Piperlonguminine and <em>o</em>-Nitrophenyl Fluorone[J]. Chinese Journal of Luminescence, 2012,(5): 558-561 DOI： 10.3788/fgxb20123305.0558.

摘要

建立了一种测定荜茇宁的方便快捷的荷移分光光度法。荜茇宁和邻硝基苯基荧光酮在中性三酸缓冲液中发生电荷转移反应

荷移络合物在515 nm处有最大吸收

表观摩尔吸光系数为6.57110

Lmol

-1

。该络合物的组成是1:1

药物质量浓度在0.054 6~10.92 g/mL范围内服从比尔定律

检出限为0.015 3 g/mL。对反应机理进行了初步探索

并利用本方法对生物样品中的荜茇宁进行了加标回收

回收率为99.8%~100.9%

相对标准偏差(RSD)为0.90%。

Abstract

A rapid and convenient spectrophotometric method for the determination of piperlonguminine was established in the present work. The method utilized charge transfer reaction between piperlonguminine and

-nitrophenyl fluorone in pH 7.00 buffer solution. The maximum absorption wavelength of the charge transfer complex was at 515 nm and the apparent molar absorptivity was 6.57110

Lmol

-1

with a 1 ∶1 stoichiometry. Beers law was obeyed in the range of 0.054 6~10.92 g/mL of piperlonguminine with the detection limit of 0.015 3 g/mL. The reaction mechanism was discussed preliminarily. The proposed method was applied to the biological and urine samples spiked with standard piperlonguminine solution. The recovery of the samples was within the range of 99.8%~100.9% and relative standard deviation (RSD) was 0.90%.

关键词

电荷转移反应邻硝基苯基荧光酮荜茇苧分光光度法

Keywords

charge transfer reactiono-nitrophenyl fluoronepiperlongumininespectrophotometry

references

Ma Chunjie, Borjihan Gerelu, Hurilebagen, et al. Antiatherogenic effect of piperlonguminine on experimental atherosclerosis in rabbits [J]. China Journal of Chinese Materia Medica (中国中药杂志), 2008, 33(4):436-440 (in Chinese).[2] Du Licheng, He Ping, Li Xiaohong, et al. Determination of neodymium using fluorimetric liquid method [J]. Chin. J. Lumin.(发光学报), 2010, 31(6):936-941 (in Chinese).[3] Ling Xiao, Borjihan Gerelu, Zhou Haijun, et al. Fluorescence characteristics and analytical application of Mn(Ⅱ)-pipe-rine-cetyltrimethylammonium bromide ternary system [J]. Spectroscopy and Spectral Analysis (光谱学与光谱分析), 2009, 1(3):188-190 (in Chinese).[4] Xue Yonglin, Gao Ling, Pen Shouxin. Determination based on chemometricspectral geminated vitamin B1,B2 and B6 [J]. Chin. J. Lumin.(发光学报), 2011, 32(10):1081-1087 (in Chinese).[5] Pandeeswaran M, Elango K P. Spectroscopic studies on the interact ion of cimetidine drug with biologically significant and acceptors [J]. Spectrochimica Acta Part A, 2010, 75:1462-1469.

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