利用铕(Ⅲ)-诺氟沙星-十二烷基硫酸钠的 光致发光及稀土敏化荧光法测定硫酸新霉素的浓度

刘保生; 闫潇娜; 种宝红; 曹世娜; 吕运开

doi:10.3788/fgxb20123304.0453

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利用铕(Ⅲ)-诺氟沙星-十二烷基硫酸钠的光致发光及稀土敏化荧光法测定硫酸新霉素的浓度

光谱分析 | 更新时间：2020-08-12

- 利用铕(Ⅲ)-诺氟沙星-十二烷基硫酸钠的光致发光及稀土敏化荧光法测定硫酸新霉素的浓度
- Determination of Trace Neomycin Sulfate by Photoluminescence and Lanthanide Sensitized Fluorescence Quenching on Europium-norfloxacin-SDS
- 发光学报 2012年33卷第4期页码：453-457
- 作者机构：
  
  河北大学化学与环境科学学院药物化学与分子诊断教育部重点实验室,河北保定,071002
- 作者简介：
- 基金信息：
  
  国家自然科学基金(20675024)();河北省重点基础研究项目(10967126D)资助()
- DOI：10.3788/fgxb20123304.0453
  中图分类号： O657.3
- 纸质出版日期：2012-4-10，
  
  网络出版日期：2012-4-10，
  
  收稿日期：2011-12-13，
  
  修回日期：2012-1-20，
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刘保生, 闫潇娜, 种宝红, 曹世娜, 吕运开. 利用铕(Ⅲ)-诺氟沙星-十二烷基硫酸钠的光致发光及稀土敏化荧光法测定硫酸新霉素的浓度[J]. 发光学报, 2012,33(4): 453-457

LIU Bao-sheng, YAN Xiao-na, CHONG Bao-hong, CAO Shi-na, LV Yun-kai. Determination of Trace Neomycin Sulfate by Photoluminescence and Lanthanide Sensitized Fluorescence Quenching on Europium-norfloxacin-SDS[J]. Chinese Journal of Luminescence, 2012,33(4): 453-457
刘保生, 闫潇娜, 种宝红, 曹世娜, 吕运开. 利用铕(Ⅲ)-诺氟沙星-十二烷基硫酸钠的光致发光及稀土敏化荧光法测定硫酸新霉素的浓度[J]. 发光学报, 2012,33(4): 453-457 DOI： 10.3788/fgxb20123304.0453.

LIU Bao-sheng, YAN Xiao-na, CHONG Bao-hong, CAO Shi-na, LV Yun-kai. Determination of Trace Neomycin Sulfate by Photoluminescence and Lanthanide Sensitized Fluorescence Quenching on Europium-norfloxacin-SDS[J]. Chinese Journal of Luminescence, 2012,33(4): 453-457 DOI： 10.3788/fgxb20123304.0453.

摘要

室温下

在pH=7.4的氨-氯化铵缓冲溶液中

铕(Ⅲ)与诺氟沙星反应形成稳定络合物

产生稀土敏化荧光

其最佳激发、发射波长分别为

=340 nm、

=612 nm

表面活性剂十二烷基硫酸钠(SDS)的加入使其荧光强度进一步增加。在该反应体系中加入适量硫酸新霉素溶液

铕(Ⅲ)与诺氟沙星络合物的激发、发射峰位置不变

但荧光强度降低

据此建立了灵敏的测定硫酸新霉素的荧光分析方法。硫酸新霉素的浓度在0.909~22.72 mg/L范围内符合线性关系。该方法的检出限为0.40 mg/L

可用于片剂及滴眼液中硫酸新霉素含量的测定

快速、简便、结果满意。6次平行测定的回收率为90.5%~102.3%

相对标准偏差为1.4%~3.5%。

Abstract

In the ammonia-ammonium chloride buffer solution of pH=7.4

europiumion(Eu

) and norfloxacin(NFLX) react and combine as steady complex under room temperature. The complex shows its sensitized fluorescence of rare earth. The maximum excitation wavelength and maximum emission wavelength are 340 nm and 612 nm

respectively. The fluorescence intensity has a further increase in sodium dodecyl sulfate(SDS). When neomycin sulfate solution is added

the fluorescence intensity of the system of Eu

-NFLX-SDS diminishes

while the locations of the excitation and emission wavelengths stay the same. According to this result

a new way to determine the concentration of neomycin sulfate is established. The concentration range that neomycin sulfate can detect is 0.909~22.7 mg/L. The detection limit is up to 0.4 mg/L. This measurement has been applied to determine neomycin sulfate in tablet and drops. The relative standard deviation is 1.4%~3.5% for 6 parallel determination of neomycin sulfate samples and the recovery is 90.5%~102.3%.

关键词

硫酸新霉素铕诺氟沙星十二烷基硫酸钠荧光猝灭

Keywords

neomycin sulfateeuropiumionnorfloxacinsodium dodecyl sulfatefluorescence quenching

references

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