基于双发射碳点的荧光探针构建及对水中铜离子的检测

李如雪; 黄明玥; 周硼; 段春迎

doi:10.37188/CJL.20230133

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基于双发射碳点的荧光探针构建及对水中铜离子的检测

更新时间：2023-06-14

- 基于双发射碳点的荧光探针构建及对水中铜离子的检测
  增强出版
- Construction of Fluorescence Probe Based on Carbon Dots Dual Emission Strategy and Ratio Detection of Copper Ions in Water
- 发光学报 2023年页码：1-9
- 作者机构：
  
  1.大连理工大学化学学院，辽宁大连　116024
- 作者简介：
  
  [ "李如雪（1998－），女，山东济宁市，硕士研究生，学生，2020年于山东师范大学获得学士学位，主要从事荧光探针的研究。 E-mail： lir857357320@163.com" ]
  [ "周硼(1965－)，女，辽宁大连人，博士研究生，副教授，2003年于大连理工大学获得博士学位，主要从事荧光探针的研究。 E-mail: zhoupeng@dlut.edu.cn" ]
- 基金信息：
  
  国家重大仪器设备开发专项(2012YQ090197);国家自然科学基金(52070032)
- DOI：10.37188/CJL.20230133
  中图分类号：
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李如雪, 黄明玥, 周硼, 等. 基于双发射碳点的荧光探针构建及对水中铜离子的检测[J/OL]. 发光学报, 2023,1-9.

LI Ruxue, HUANG Mingyue, ZHOU Peng, et al. Construction of Fluorescence Probe Based on Carbon Dots Dual Emission Strategy and Ratio Detection of Copper Ions in Water[J/OL]. Chinese Journal of Luminescence, 2023,1-9.
李如雪, 黄明玥, 周硼, 等. 基于双发射碳点的荧光探针构建及对水中铜离子的检测[J/OL]. 发光学报, 2023,1-9. DOI： 10.37188/CJL.20230133.

LI Ruxue, HUANG Mingyue, ZHOU Peng, et al. Construction of Fluorescence Probe Based on Carbon Dots Dual Emission Strategy and Ratio Detection of Copper Ions in Water[J/OL]. Chinese Journal of Luminescence, 2023,1-9. DOI： 10.37188/CJL.20230133.

摘要

为克服测试时环境变化对荧光检测的影响，我们设计合成比率型荧光探针，利用双波长的荧光发射来有效消除背景干扰。本文以邻苯二胺、四硼酸钠和1-甲基-3-烯丙基咪唑溴盐为反应前体，利用一步水热法合成了基于碳点双发射的荧光探针L-CDs，并实现了对铜离子（Cu,2+,）的双信号响应。L-CDs表现出荧光双发射现象，当激发波长为380 nm时，呈现出440 nm和570 nm的双发射峰。Cu,2+,可使探针在440 nm处的荧光发射强度减弱，同时570 nm的荧光发射峰增强。Cu,2+,浓度在0.04-0.244 mM范围内时，与荧光比率信号（F,570,/F,440,）表现出良好的线性相关，检出限（LOD）为0.6 μM。所构建的荧光探针可用于实际水样中Cu,2+,的检测，回收率为99.4 ~ 101.8%。

Abstract

In order to overcome the influence of environmental change on fluorescence detection， a ratio fluorescence probe was designed and prepared， and dual-wavelength fluorescence emission was used to effectively eliminate background interference. Using o-phenylenediamine， sodium tetraborate and 1-methyl-3-allyl imidazolium bromide as precursors， a one-step hydrothermal method has been developed to synthesize a ratio fluorescent probe based on carbon dots （L-CDs）， and to achieve a double signal response to heavy metal ions （Cu,2+,）. L-CDs exhibited fluorescence double emission phenomenon， and showed double emission peaks at 440 nm and 570 nm when the excitation wavelength was 380 nm. The addition of Cu,2+, can reduce the fluorescence emission intensity at 440 nm and increase the fluorescence emission peak value at 570 nm. The concentration of Cu,2+, in the range of 0.04-0.244 mM showed a good linear correlation with the fluorescence ratio signal （F,570,/F,440,）， and the detection limit （LOD） was 0.6 μM. The fluorescence probe can be used for the detection of Cu,2+, in actual water samples with the recovery of 99.4 ~ 101.4%.

关键词

碳量子点荧光探针铜离子比率探针

Keywords

carbon dotsFluorescence Probecupper ionRatio probe

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