环丙沙星、氧氟沙星同时 与牛血清白蛋白分子作用的荧光光谱

刘保生; 薛春丽; 王 晶; 杨 超; 吕运开

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环丙沙星、氧氟沙星同时与牛血清白蛋白分子作用的荧光光谱

论文 | 更新时间：2020-08-12

- 环丙沙星、氧氟沙星同时与牛血清白蛋白分子作用的荧光光谱
- Studies on the Interaction between Ciprofloxacin and Ofloxacin in Presence of Bovine Serum Albumin by Spectroscopic Methods
- 发光学报 2010年31卷第2期页码：285-290
- 作者机构：
  
  河北大学理化分析中心药物化学与分子诊断教育部重点实验室,河北保定,071002
- 作者简介：
- 基金信息：
- DOI：
  中图分类号： O657.3;O482.31
- 纸质出版日期：2010-4-30，
  
  网络出版日期：2010-4-30，
  
  收稿日期：2009-11-25，
  
  修回日期：1900-1-2，
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刘保生, 薛春丽, 王晶, 等. 环丙沙星、氧氟沙星同时与牛血清白蛋白分子作用的荧光光谱[J]. 发光学报, 2010,31(2):285-290.

LIU Bao-sheng, XUE Chun-li, WANG Jing, et al. Studies on the Interaction between Ciprofloxacin and Ofloxacin in Presence of Bovine Serum Albumin by Spectroscopic Methods[J]. Chinese Journal of Luminescence, 2010,31(2):285-290.
刘保生, 薛春丽, 王晶, 等. 环丙沙星、氧氟沙星同时与牛血清白蛋白分子作用的荧光光谱[J]. 发光学报, 2010,31(2):285-290. DOI：

LIU Bao-sheng, XUE Chun-li, WANG Jing, et al. Studies on the Interaction between Ciprofloxacin and Ofloxacin in Presence of Bovine Serum Albumin by Spectroscopic Methods[J]. Chinese Journal of Luminescence, 2010,31(2):285-290. DOI：

摘要

在pH=7.40的水溶液中

环丙沙星(CPFX)、氧氟沙星(OFLX)能够猝灭牛血清白蛋白(BSA)的荧光。当两种药物共存时BSA荧光被进一步猝灭。据此建立了利用荧光发光光谱法进行喹诺酮类药物CPFX与OFLX间相互作用的研究。结果表明:药物间存在相互作用

使药物与蛋白间的结合常数减小、结合稳定性下降

游离型药物含量增加

造成药效增强;药物对蛋白荧光的猝灭属于静态猝灭

药物与蛋白结合位点数约为1。根据Frster非辐射能量转移理论

确定了药物与蛋白之间的结合距离

＜7 nm

属于非辐射能量转移。药物间的相互作

用使r

值增加

结合距离增大。同步荧光光谱研究表明药物间的相互作用对蛋白构象产生影响

使蛋白质分子伸展

疏水性降低。

Abstract

In the aqueous solution of pH=7.40

both ciprofloxacin (CPFX) and ofloxacin (OFLX) can quench fluorescence of bovine serum albumin (BSA). The fluorescence intensity is obviously gone down when the two drugs coexist. According to this

studies were established on the interaction between CPFX and OFLX by fluorescence spectrum. It was proved that the interaction between the drugs reduces the binding stability of the drug and protein. At the same time

increase of the free drug concentration will increase the effect of the drugs. The result showed that the quenching mechanism of the combination of bovine serum albumin and drugs is a static procedure. The number of binding sites is 1 in several of system. Based on the theory of Frester spectroscopy energy transfer

the binding distance (

＜7.0 nm) between the drugs and BSA was obtained. It belongs to non-radiation energy transfer. Because of the existence of interaction between the drugs

the correlation coefficient and binding distance are increased. Studies by synchronous spectra show the effect of the interaction between the drugs on conformation of BSA. It makes protein molecules extend and hydrophobic nature reduces.

关键词

环丙沙星氧氟沙星牛血清白蛋白荧光光谱相互作用

Keywords

ciprofloxacinofloxacinbovine serum albuminfluorescence spectrainteraction

references

. Shao Shuang, Ma Boying, Wang Xuejie, et al. Study on the interaction between cefodium sodium and serum albumin [J]. Acta Phys. Chim. Sin. (物理化学学报), 2005, 21 (7):792-795 (in Chinese).

. Mathias U, Jung M. Determination of drug-serum protein interactions via fluorescence polarization measurements [J]. Anal. Bioanal. Chem., 2007, 388 (5-6):1147-1156.

. Rus O B, Puchkaev A V, Ivanov A I, et al. Interaction of albumins and hemin with aromatic antioxidants: A spectrophotometric and fluorometric study [J]. Appl. Biochem. Micro., 2006, 36 (1):36-45.

. Smyk B. Fluorescence study of sinapic acid interaction with bovine serum albumin and egg albumin [J]. J. Fluoresc., 2003, 13 (4):349-356.

. Bai Haixin, Yang Cheng, Yang Xiurong. Interaction between bovine serum albumin and Indo-1 using fluorescence spectroscopic method [J]. Chem. J. Chinese Univ. (高等学校化学学报), 2007, 28 (2):227-332 (in Chinese).

. Feng Xizeng, Bai Chunli, Lin Zhang.The interaction between acridine orange and bovine serum albumin [J]. Anal.Chem. (分析化学), 1998, 26 (2):154-157 (in Chinese).

. Long Xiufen, Liu Shaopu, Kong Ling, et al. A study on the interaction of proteins with some heteropoly compounds and their analytical application by resonance Rayleigh scattering method [J]. Talanta, 2004, 63 (2):279-286.

. Gong Aiqin, Zhu Xiashi, Hu Yanyan, et al. A fluorescence spectroscopic study of the interaction between epristeride and bovine serum albumin and its analytical application [J]. Talanta, 2007, 73 (4):668-673.

. Zhang Qingzhu. Fundamental Pharmacology [M]. Beijing: Higher Education Press, 2006, 48.

. Zhou Xiumei, Yang Wenbin, Chai Qiuyan. Studies of general pharmacological experiment on danshen injection [J]. Shanxi Med. J. (山西医药学杂志), 2004, 33 (12):1028-1030 (in Chinese).

. Li Xin, Gong Zehui, Chi Mugen, et al. Screening of ET-1receptor antagonists and the pharmacological evaluation [J]. Chin. Pharmacological Bulletin. (中国药理学通报), 2008, 24 (11):1422-1426 (in Chinese).

. Zhang Xiaowei, Zhao Fenglin, Li Kean. Studies on the reaction between ciprofloxacin and bovine serum albumin [J]. Chem. J. Chin. Univ. (高等学校化学学报), 1999, 20 (7):1063-1067 (in Chinese).

. Zou Jun,Li Xinxin,Yang Zhaoxu, et al. In vitroactivities of levofloxacin against helicobacter pylori [J]. J. Chin. Anti-biotics. (中国抗生素杂志), 2003, 28 (5):292-294 (in Chinese).

. Zhang Hongmei, Wang Yangqing, Yu Liming, et al. Spectroscopic study on interaction between zinc phthalocya-nine and bovine hemoglobin (BHb) [J]. Chemical Reagents (化学试剂), 2008, 30 (2):85-88 (in Chinese).

. Wei Yanli, Li Jianqing, Dong Chuan, et al. Investigation of the association behaviors between biliverdin and bovine serum albumin by fluorescence spectroscopy [J]. Talanta, 2006, 70 (2):377-382.

. Sun Yantao, Zhang Haitao, Sun Ye, et al. Study of interaction between protein and mainactive components in citrus aurantium L. by optical spectroscopy [J]. J. Lumin., 2010, 130 (2):270-279.

. Jiang Zhiqiang, Chi Yanhua, Zhuang Jia, et al. Mechanism studies on the combination Reaction between bovine serum albumin and zincon by fluorescence spectra [J]. Spectroscopy and Spectral Analysis (光谱学与光谱分析), 2007, 27 (5):986-990 (in Chinese).

. Pu Yingqiu, Tao Weibo. Studies on the interaction between seratrodastand bovine serum album in by fluorescence spectroscop [J]. Phamaceutical and Clinical Research (药学与临床研究), 2007, 15 (4):287-291 (in Chinese).

. Ge Feng, Wang Jianping, Liu Diqiu, et al. Study on interaction between shikonin and bovine serum albumin by fluorescence spectra [J]. The Chinese Journal of Process Engineering (过程工程学报), 2009, 9 (1):118-122 (in Chinese).

. Huang Bo, Zou Guolin, Yang Tianming. Studies on the interaction between adriamycin and bovine serum albumin [J]. Acta Chimica Sinica (化学学报), 2002, 60 (10):1867-1871 (in Chinese).

. Wu Xiaohong, Zhou Jiahong, Gu Xiaotian, et al. Study on interaction between hypocrellin A and hemoglobin or myoglobin using synchronous fluorescence spectra [J]. Spectroscopy and Spectral Analysis (光谱学与光谱分析), 2006, 26 (12): 2287-2290 (in Chinese).

. Chen Daiwu, Xie Qingji, Jiang Xueqin, et al. Inreraction of quercetion with casein and bovine serum albumin as well as effects of coexisting carbon nanotubes [J]. Acta Phys. Chim. Sin. (物理化学学报), 2008, 24 (3):379-387 (in Chinese).

. Yan Chengnong, Zhang Huaxin, Liu Yi, et al. Fluorescence spectra of the binding reaction between paraquat and bovine serum albumin [J]. Acta Chimica Sinica (化学学报), 2005, 63 (18):1727-1732 (in Chinese).

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