Preparation and Properties of SiO2 Coating Europium (Ⅲ) Complex Fluorescent Nanoparticles

ZHOU Li-rong; ZHOU Li-qun; YU Guo-feng; TANG Zi-wei; WANG Fen

doi:null

您当前的位置：

首页 >

文章列表页 >

Preparation and Properties of SiO₂ Coating Europium (Ⅲ) Complex Fluorescent Nanoparticles

更新时间：2020-08-12

- Preparation and Properties of SiO₂ Coating Europium (Ⅲ) Complex Fluorescent Nanoparticles
- Chinese Journal of Luminescence Vol. 29, Issue 2, Pages: 371-375(2008)
- 作者机构：
  
  1. 湖北大学, 有机功能分子合成与应用教育部重点实验室,湖北武汉,430062
  2. 孝感学院, 化学系,湖北孝感,432100
- 作者简介：
- 基金信息：
- DOI：
  CLC： O482.31;O614.33
- Published：20 March 2008，
  
  Received：19 July 2007，
  
  Revised：28 December 2007，
扫描看全文
周丽荣, 周立群, 余国锋, 唐子威, 王芬. SiO2包覆铕(Ⅲ)配合物的荧光纳米粒子合成与性质[J]. 发光学报, 2008,29(2): 371-375

ZHOU Li-rong, ZHOU Li-qun, YU Guo-feng, TANG Zi-wei, WANG Fen. Preparation and Properties of SiO2 Coating Europium (Ⅲ) Complex Fluorescent Nanoparticles[J]. Chinese Journal of Luminescence, 2008,29(2): 371-375
周丽荣, 周立群, 余国锋, 唐子威, 王芬. SiO2包覆铕(Ⅲ)配合物的荧光纳米粒子合成与性质[J]. 发光学报, 2008,29(2): 371-375 DOI：

ZHOU Li-rong, ZHOU Li-qun, YU Guo-feng, TANG Zi-wei, WANG Fen. Preparation and Properties of SiO2 Coating Europium (Ⅲ) Complex Fluorescent Nanoparticles[J]. Chinese Journal of Luminescence, 2008,29(2): 371-375 DOI：

摘要

以前驱物pAB-DTPAA-APTEOS、正硅酸乙酯(TEOS)和三氯化铕(EuCl3)等为原料

采用油包水(W/O)的反相微乳液法

在正硅酸乙酯(TEOS)和3-氨丙基三乙氧基硅烷(APTEOS)的共同水解下

制备出新型的SiO

包覆铕配合物荧光纳米粒子Eu-pAB-DTPAA-AP-SiO

。运用TEM、IR、UV-Vis、荧光光谱等技术对荧光纳米粒子进行了表征。TEM结果表明:包覆体呈球形

分散均匀

平均粒径为40nm。纳米粒子与配体、前驱物的紫外吸收谱相比较

峰位发生了一定的红移

表明通过反相微乳液法得到的固体粉末与EuCl3反应后

已经生成配合物Eu-pAB-DTPAA-AP-SiO

。红外光谱研究表明

在801cm-1出现ν

Si-C

的伸缩振动峰

471cm-1处出现ν

Eu-O

的伸缩振动峰。由此证实Eu-pAB-DTPAA-AP-SiO

配合物的存在。荧光光谱分析表明

纳米粒子Eu-pAB-DTPAA-AP-SiO

表现出较好的荧光性能

位于592

615

689nm的发射峰分别归属于Eu

离子的

→

、

→

和

→

跃迁

其中最强峰615nm属于Eu

的特征跃迁发射。作为一种新型的荧光试剂

该纳米粒子具有粒径小

亲水性强

荧光强度大

且表面的氨基能方便地与生物分子偶联

故可作为优良的时间分辨荧光标记物用于各种高灵敏生物检测技术中。

Abstract

The incorporation of rare earth complex into inorganic hybrid host materials has been extensively explored in recent studies

especially focused on SiO

-based materials

and the obtained materials were found to show high improvements for their thermal

mechanical properties and chemical stability. Furthermore

the materials are nanometer-sized luminescent

and become a research attractions as luminescence probes in various types of biological detection.In this paper

the fluorescent nanoparticles were prepared with pAB-DTPAA-APTEOS precursor

TEOS and EuCl3 as raw materials by means of water-in-oil (W/O) microemulsion through controlling copolymerization of tetraethyl orthosilicate (TEOS) and 3-aminopropyl-triethyloxysilane (APTEOS). Fluorescent nanoparticles were characterized with TEM

UV-vis

IR and fluorescence techniques. It can be seen from the TEM image of the phosphor that the fluorescent nanoparticles are spherical and better particle dispersity

with average particle size of 40 nm. The IR spectra of Eu-pAB-DTPAA-AP-SiO

has two regions

which correspond to Si-C stretching (801 cm

-1

) and Eu-O stretching (471 cm

-1

)

this confirms the existence of complex Eu-pAB-DTPAA-AP-SiO

. In the UV-vis spectra

compared to the absorption spectrum of pAB-DTPAA

a red shift of the first peak (from 232 nm to 260 nm) was found in the spectrum of the precursor (pAB-DTPAA-APTEOS)

no change was observed for the major at 336 nm

which indicates the information of pAB-DTPAA-APTEOS. Furthermore

after EuCl3 was added to the solution of pAB-DTPAA-APTEOS

the red shift phenomena of the absorption peaks were observed. These changes indicate that complex Eu-pAB-DTPAA-AP-SiO

was formed in the pAB-DTPAA-APTEOS-EuCl3 solution. The excitation and emission spectra of the nanoparticles indicate that the excitation peak wavelength is at 260 nm and the emission peak wavelength is at 615 nm. When the nanoparticles were excited by 260 nm

only the emission lines of

→

(J= 1～4) of Eu

were observed

with the hypersensitive

→

transition as the most prominently single radiation peak without splitting. As a new analytical reagent

the fluorescent nanoparticles combine the advantages of luminophore-doped silica nanoparticle probe and lanthanide latex fluorescence probe including smaller size (about 40 nm)

high hydrophilicity and biocompatibility. Furthermore

the amino groups directly introduced to the nanoparticles surface by using APTEOS in the preparation made the surface modification and bioconjugation of the nanoparticles easier. The particles are potential of good biocompatibility and can be excepted as efficient biological labels.

关键词

SiO2铕配合物微乳液荧光纳米粒子包覆

Keywords

SiO2europium complexmicroemulsionfluorescent nanoparticlescoating

references

Tang Aiwei,Teng Feng,Gao Yinhao,et al.Synthesis and luminescent properties of Core/shell/shell structural CdSe/CdS/ZnS nanocrystals[J].Chin.J.Lumin.(发光学报),2006,27(2):234-238 (in Chiense).

Chao Kefu,Zhang Youlin,Kong Xiangqui,et al.A study on fluorescence spectroscopy of immobilizing FITC labeled antibody between fiber optic surface and biomolecule[J].Chin.J.Lumin.(发光学报),2007,28(6):940-944 (in Chinese).

Patolsky F,Gill R,Weizmann Y,et al.Lighting-up the dynamics of telomerization and DNA replication by CdSe-ZnS quantum dots[J].J.Am.Chem.Soc.,2003,125(46):13918-13919.

Harma H,Soukka T,Lonnberg S,et al.Zeptomole detection sensitivity of prostate-specific antigen in a rapid microtitre plate assay using time-resolved fluorescence[J].Lumin.,2000,15(6):351-355.

Vaisanen V,Harma H,Lilja H,et al.Time-resolved fluorescence imaging for quantitative histochemistry using lanthanide chelates in nanoparticles and conjugated to monoclonal antibodies[J].Lumin.,2000,15(6):389-397.

Harma H,Soukka T,Lovgren T.Europium nanoparticles and time-resolved fluorescence for ultrasensitive detection of prostate-specific antigen[J].Clin.Chem.,2001,47(3):561-568.

Santra S,Zhang P,Wang K,et al.Conjugation of biomolecules with luminophore-doped silica nanoparticles for photostable biomarkers[J].Anal.Chem.,2001,73(20):4988-4993.

Santra S,Wang K,Tapec R,et al.Development of novel dye-doped silica nanoparticles for biomarker application[J].J.Biomed.Opt.,2001,6(2):160-166.

Hilliard L R,Zhao X,Tan W.Immobilization of oligonucleotides onto silica nanoparticles for DNA hybridization studies[J].Anal.Chim.Acta,2002,470(1):51-56.

Qhobosheane M,Santra S,Zhang P,et al.Biochemically functionalized silica nanoparticles[J].Analyst,2001,126(8):1274-1278.

Santra S,Tapec R,Theodoropoulou N,et al.Synthesis and characterization of silica-coated iron oxide nanoparticles in microemulsion:the effect of nonionic surfactants[J].Langmuir,2001,17(10):2900-2906.

Soini E,Lovgren T.Time-resolved fluorescence of lanthanide probes and applications in biotechnology[Review].CRC Crit.Rev.Anal.Chem.,1987,18:105-154.

Gudgin Dickson Eva F,Alfred Pollak,Diamandis E P.Ultrasensitive bioanalytical assays using time-resolved fluorescence detection[J].Pharmacol.Ther.,1995,66(2):207-235.

Diamandis E P.Immunoassay with time-resolved fluorescence spectroscopy:principles and applications[J].Clin.Biochem.,1988,21(3):139-150.

Yuan J L,Wang G L,Majima K,et al.Synthesis of a terbium fluorescent chelate and its application to time-resolved fluoroimmunoassay[J].Anal.Chem.,2001,73(8):1869-1876.

Ning Q Y,Meng J X,Wang H M.Preparation of novel terbium(Ⅲ) chelate-doped fluorescent silica nanoparticles[J].J.the Chinese Rare Earth Society (中国稀土学报),2006,3(24):289-292 (in Chinese)

Wang X M,Kong W,Xu H.Preparation of EDTA Anhydride and DTPA Anhydride[M].Zhengzhou:Henan Chemical Industry,1999,14-15 (in Chinese)

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Synthesis and Characterization of Core-shell Structured YVO₄ :Eu³⁺@SiO₂ Nanocomposites

Controllable Synthesis and Surface Modification of Fe₃O₄ Nanoparticles

Preparation of La₂(MoO₄)₃ by A Simple Microemulsion Method and Its Photoluminescence Properties

Preparation and Characterization of Fluorescent Hybrid Nanospheres of SiO₂/Rhodamine B via Microemulsion

Preparation and Characterization of LaPO₄ ∶ Eu³⁺@SiO₂ Core-shell Luminescent Naonrods

Related Author

LIU Li-na

ZHANG Dong-mei

ZHANG Yong-sheng

ZANG Chun-he

TANG Chun-juan

QIN Rui-fei

SUN Rui-rui

XIAO Hong-yu

Related Institution

Department of Mathematics and Physics, Luoyang Institute of Science and Technology

Key Laboratory of Functional Molecule Design and Interface Process, Anhui Jianzhu University

Materials and Chemical Engineering, Anhui Jianzhu University

School of Chemical Engineering, Hefei University of Technology

Address：No.3888 Dong Nanhu Road, Changchun, Jilin, China Postal code：130033
Tel：0431-86176862 Email：fgxbt@126.com
Technical support is provided by Beijing Founder electronics co., LTD 吉ICP备11002662号-17 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰