FENG Bin, TENG Feng, TANG Ai-wei, WANG Yan, HOU Yan-bing, WANG Yong-sheng. Application of Aminophenol-stabilized CdSe Nanocrystals as a Fluorescent Label for Lysozyme[J]. Chinese Journal of Luminescence, 2007,28(3): 421-424,
FENG Bin, TENG Feng, TANG Ai-wei, WANG Yan, HOU Yan-bing, WANG Yong-sheng. Application of Aminophenol-stabilized CdSe Nanocrystals as a Fluorescent Label for Lysozyme[J]. Chinese Journal of Luminescence, 2007,28(3): 421-424,DOI:
Application of Aminophenol-stabilized CdSe Nanocrystals as a Fluorescent Label for Lysozyme
Water-soluble CdSe nanocrystals were synthesized in the system of Na
2
SeSO
3
and Cd(CH
2
COO)
2
by using L-cysteine hydrochloride (L-Cys) as a stabilizer. Lysozyme (Lys) was labelled by the CdSe nanocrystals to investigate the application of CdSe nanocrystals as a fluorescent label which can be connected to the biologic molecule easily by the L-Cys over around the nanocrystals. There are more choice to label the biologic reagents because of the-NH
2
and-COOH of Lys. The powder of CdSe nanocrystals was characterized by transmission electron microscopy (TEM) and the optical properties was investigated by fluorescent spectral analysis and fluorescent microscope. It was proved that different CdSe nanocrystals with different sizes and optical pro-perties can be synthesized in different reaction condition. The peak wavelengthes of emission and absorption spectra of the CdSe nanocrystals are at about 530 and 410nm
respectively. After labeling
the full width at half maximum (FWHM) of CdSe nanocrystals can be kept well which was about 40nm and easy to distinguish during labeling the biologic reagents. Comparing with the optical properties before labelling
the emission maxima of the obtained CdSe nanocrystals have a little shift
just about 2nm
but the emission intensity of CdSe nanocrystals-labelled lysozyme can took a great enhancement about 100% with the change of the consistence of Lys. In sum
the L-cys-stabilized CdSe has an important value as a fluorescent label.
Epifani M,Giannini C.A novel synthesis of CdSe nanocrystals[J].Mater.Lett.,2004,58(19):2429-2432.
Sondi I,Siiman O.Synthesis of CdSe nanoparticles in the presence of aminodextran as stabilizing and capping agent[J].Colloid Int.Sci.,2004,275:503-507.
Zhong Huaizhen,Li Guoqiang,He Xiaoyun,et al.Preparation of nanosize CdS/PANI film and studies of its photoelectric properties[J].Chin.J.Lumin.(发光学报),2004,25(5):585-590 (in Chinese).
Wang Yinshu,Zheng Dong,Sun Ping,et al.Photoluminescence of CdSeS nanocrystals at room temperature[J].Chin.J.Lumin.(发光学报),2003,24(2):139-143 (in Chinese).
Bruchez M Jr,Moronne M,Gin P,et al.Semiconductor nanocrystals as fluorescent biological labels[J].Science,1998,281(5385):2013-2016.
Warren C,Nie Shuming.Quantum dot bioconjugates for ultrasensitive nonisotopic detection[J].Science,1998,281(5385):2016-2018.
Han M Y,Gao X H,Su J Z,et al.Quantum-dot-tagged micro-beads for multiplexed optical coding of biomolecules[J].Nature Biotech.,2001,19(7):631-635.
Lin Zhangbi,Zhang Hao,Jin Qinhan,et al.Studies on labeling of papain with quantum dots synthesized in aqueous-solution[J].Chem.J.Chin.Univ.(高等学校化学学报),2003,24(4):609-611 (in Chinese).
Talapin D V,Haubold S,Rogach A L,et al.A novel organometallic synthesis of highly luminescent CdTe nanocrystals[J].Phps.Chem.B,2001,105(12):2260-2263.
Aldana J,Wang Y A,Peng X,et al.Photochemical instability of CdSe nanocrystals coated by hydrophilic thiolsf[J].J.Am.Chem.Soc.,2001,123(36):8844-8850.
Lin Cunhua,Xiao Surong,Meng Qingguo.The structure and characteristic of lysozyme and its application[J].J.Weifang University (潍坊学院学报),2005,5(2):108-110 (in Chinese).
Yu Ying,Zhou Zhentang.Fluorescence determination of lysozyme with cadmium di-selenide/cadmium sulfide quantum dots synthesized in the aqueous solution[J].Chin.J.Anal.Chem.(分析化学),2005,33(5):650-652 (in Chinese).
Tang Aiwei,Teng Feng,Gao Yinhao,et al.Influence of preparation conditions of CdSe nanocrystals on luminescence properties[J].Chin.J.Lumin.(发光学报),2005,26(5):622-626 (in Chinese).
Chen Zengxie,Liu Jing,Luo Dan.Biochemistry Experiments (生物化学实验)[M].Hefei:Press of University of Science and Technology of China,1994,29 (in Chinese).
Zhong Ping,Yu Ying,Wu Jiangzhong,et al.Preparation and application of functionalized nanoparticles of CdSe capped with 11-mercaptoundecanoic acid as a fluorescence probe[J].Talanta,2006,70(4):902-906.
Lin Zhangbi,Zhang Hao,Chen Qidan,et al.Studies on labeling of papain with quantum dots synthesized in aqueous solution[J].Chem.J.Chin.Univ.(高等学校化学学报),2003,24(4):609-611 (in Chinese).