In order to developing sensitive and precise testoterone chemiluminescence immunoassdy

optimal paremeters of measuring acridinium ester and antibody titer must be selected.In this study

we find that time paremeter for luminescence analysis testosterone-3-carboxymethyloxime-4-(To-mehty)-acridinium-9-carboxylate(To-3AEPMAC)is very important in the low concentration.During the measurement

there is a peak of solvent in 0.00～0.21s

peak of To-3-AEPMAC in 0.43～2.22s.The luminescence reaction can be completed in 0.32～3.91s.But it is not very correction because the more To-3-AEPMAC is

the longer luminescence peak time is.These results are decided by the reaction mixing condition and reactive dynamics.The solvent peak occupies 4.10±2.21%(

x

±s)in all luminescence.The same result is obtained with measuing the reaction rate.It is best to choose 0.30 s delay time and 10 s integrated time for analysis the chemiluminescence characteristic.This measuring manner riot only refused the luminescence disturbance of solvent

but also obtained all signal of To-3-AEPMAC.Then it is bad to analysis the hight peak of luminescence because of varying time in peak appearing.Optimal antibody titer is measured with three methods

there are:maximum value of antibody diluted curve

maximum verying reaction and minimum reaction standard error.The first and second methods are respected for sensition

the later method is respected for precision of standard curve.The optimal antibody titer of testosterone chemiluminescence immunoassay is 1:18000.