红枣碳量子点&ldquo;关-开&rdquo;型荧光探针检测芦丁的研究

王靖原; 张越诚; 马红燕; 刘晓莉; 王琦

doi:10.3788/fgxb20183904.0600

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红枣碳量子点“关-开”型荧光探针检测芦丁的研究

发光学应用及交叉前沿 | 更新时间：2020-08-12

- 红枣碳量子点“关-开”型荧光探针检测芦丁的研究
- Synthesized of Carbon Quantum Dots from Red Dates and as A “Off-On” Fluorescence Probe for High Sensitive Detection of Rutin
- 发光学报 2018年39卷第4期页码：600-607
- 作者机构：
  
  延安大学化学与化工学院延安市分析技术与检测重点实验室,陕西延安,716000
- 作者简介：
- 基金信息：
  
  陕西省自然科学基础研究计划（2014JM2056）();延安大学研究生科研创新项目资助()
- DOI：10.3788/fgxb20183904.0600
  中图分类号： O657.3
- 纸质出版日期：2018-4-5，
  
  网络出版日期：2017-10-25，
  
  收稿日期：2017-8-15，
  
  修回日期：2017-10-18，
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王靖原, 张越诚, 马红燕等. 红枣碳量子点“关-开”型荧光探针检测芦丁的研究[J]. 发光学报, 2018,39(4): 600-607

WANG Jing-yuan, ZHANG Yue-cheng, MA Hong-yan etc. Synthesized of Carbon Quantum Dots from Red Dates and as A “Off-On” Fluorescence Probe for High Sensitive Detection of Rutin[J]. Chinese Journal of Luminescence, 2018,39(4): 600-607
王靖原, 张越诚, 马红燕等. 红枣碳量子点“关-开”型荧光探针检测芦丁的研究[J]. 发光学报, 2018,39(4): 600-607 DOI： 10.3788/fgxb20183904.0600.

WANG Jing-yuan, ZHANG Yue-cheng, MA Hong-yan etc. Synthesized of Carbon Quantum Dots from Red Dates and as A “Off-On” Fluorescence Probe for High Sensitive Detection of Rutin[J]. Chinese Journal of Luminescence, 2018,39(4): 600-607 DOI： 10.3788/fgxb20183904.0600.

摘要

以红枣为原料，通过一步水热法合成了具有强烈荧光的碳量子点（CQDs），通过荧光光谱、紫外-可见光谱、红外光谱、X射线粉末衍射光谱对其发光特性进行了研究，并基于KMnO

与CQDs形成无辐射复合体，构建了"关-开"型荧光探针用于芦丁的高灵敏度检测。实验发现，在pH=4.00的HAc-NaAc缓冲溶液中，KMnO

的加入可使CQDs的荧光发生显著猝灭，体系的荧光信号处于"关闭"状态；加入芦丁后，由于KMnO

更易与还原性药物芦丁发生反应，将KMnO

从CQDs表面移除下来，使CQDs的荧光得以恢复，体系的荧光信号处于"打开"状态。对反应的机理进行初步探讨，考察了各种反应条件，在优化的实验条件下，CQDs荧光探针荧光恢复值

与芦丁的浓度在2.510

-7

~1.010

-5

mol/L范围内呈良好线性关系，方法检出限为1.510

-7

mol/L。该方法应用于样品中芦丁的测定，结果满意。

Abstract

A one-step hydrothermal method was developed for the preparation of carbon quantum dots(CQDs) with strong fluorescence by using red dates as precursors. The luminescence properties of the CQDs were studied by fluorescence spectroscopy

UV-Vis spectrum

transmission electron microscopy

infrared spectrum

X-ray powder diffraction spectroscopy. In pH=4.00 NaAc-HAc buffer solution

after potassium permanganate(KMnO

) was added in CQDs solution

the fluorescence of CQDs was quenched

and the fluorescence signal of the system was in the "off" state. In the presence of rutin

the fluorescence of CQDs was recovered because KMnO

preferred to react with rutin

which results in the departure of KMnO

from the surface of the CQDs

and the fluorescence signal of the system was "open". The degree of recovery of CQDs fluorescence intensity was proportional to the rutin concentration

which provided a new method for the detection of rutin with high sensitivity and selectivity based on the fluorescence quenching-recovery properties of CQDs. The mechanism of fluorescence quenching of CQDs by KMnO

was studied

and the reaction conditions were optimized. Under optimal conditions

the recovered fluorescence of CQDs is linearly related with the rutin concentration in the range 2.510

-7

-1.010

-5

mol/L

the detection limit is 1.510

-7

mol/L. The present method is applied for the detection of rutin in samples with satisfactory results.

关键词

碳量子点荧光探针芦丁高锰酸钾红枣

Keywords

carbon quantum dotsfluorescence proberutinpotassium permanganatered dates

references

XU X, RAY R, GU Y, et al.. Electrophoretic analysis and purification of fluorescent single-walled carbon nanotube fragments[J]. J. Am. Chem. Soc., 2004, 126(40):12736-12737.

SUN Y P, ZHOU B, LIN Y, et al.. Quantum-sized carbon dots for bright and colorful photoluminescence[J]. J. Am. Chem. Soc., 2006, 128(24):7756-7757.

娄庆, 曲松楠. 基于超级碳点的水致荧光"纳米炸弹"[J]. 中国光学, 2015, 8(1):91-98. LOU Q, QU S N. Water triggered luminescent "nano-bombs" based on supra-carbon-nanodots[J]. Chin. Opt., 2015, 8(1):91-98. (in Chinese)

YAN Z, SHU J, YU Y, et al.. Preparation of carbon quantum dots based on starch and their spectral properties[J]. Lumin. J. Biol. Chem. Lumin., 2015, 30(4):388-392.

PENG F U, ZHOU L, TANG L, et al.. Progress in preparation of carbon quantum dots and its application in the fields of energy and environment[J]. Chin. J. Appl. Chem., 2016, 33(7):742-755.

苏安梅, 钟青梅, 余姝轶, 等. 碳量子点荧光猝灭法测定饮料中日落黄[J]. 发光学报, 2017, 38(4):530-534. SU A M, ZHONG Q M, YU S Y, et al.. Determination of sunset yellow in beverage by fluorescence quenching method with carbon quantum dot[J]. Chin. J. Lumin., 2017, 38(4):530-534. (in chinese)

LIM S Y, SHEN W, GAO Z. Carbon quantum dots and their applications[J]. Chem. Soc. Rev., 2015, 44(1):362-381.

TYAGI A, TRIPATHI K M, SINGH N, et al.. Green synthesis of carbon quantum dots from lemon peel waste:applications in sensing and photocatalysis[J]. RSC Adv., 2016, 6(76):72423-72432.

刘晓莉, 马红燕, 任岗, 等. 菜花碳量子点的一步合成及其与塞克硝唑的相互作用[J]. 分析试验室, 2017, 36(7):768-771. LIU X L, MA H Y, REN G, et al.. One-step synthesis of cauline carbon quantum dots and its interaction with secnidazole[J]. Chin. J. Anal. Lab., 2017, 36(7):768-771. (in Chinese)

张玉娟, 赵智慧, 王英特, 等. 碳量子点的绿色合成及检测水体中苦味酸[J]. 分析试验室, 2016, 35(11):1259-1263. ZHANG Y J, ZHAO Z H, WANG Y T, et al.. Carbon synthesis of carbon dots and detection of picric acid in water[J]. Chin. J. Anal. Lab., 2016, 35(11):1259-1263. (in Chinese)

DU W, XU X, HAO H, et al.. Green synthesis of fluorescent carbon quantum dots and carbon spheres from pericarp[J]. Sci. Chi. Chem., 2015, 58(5):863-870.

SUN W, YANG M, LI Y, et al.. Electrochemical behavior and determination of rutin on a pyridinium-based ionic liquid modified carbon paste electrode[J]. J. Pharm. Biomed. Anal., 2008, 48(5):1326-1331.

LIU D, MEI Q, WAN X, et al.. Determination of rutin and isoquercetin contents in hibisci mutabilis folium in different collection periods by HPLC[J]. J. Chromatog. Sci., 2015, 53(10):1680-1684.

JANGDE R K, SINGH M, SINGH D. Development and determination of rutin by spectrofluorimetric method[J]. Biomed. Pharm. J., 2015, 7(2):681-686.

GAO Y, WANG L, ZHANG Y, et al.. Greenly synthesized graphene with L-glutathione modified electrode and its application towards determination of rutin[J]. RSC Adv., 2016, 6(96):94024-94032.

SUN Y, GUO T, SUI Y, et al.. Quantitative determination of rutin, quercetin, and adenosine in flos carthami by capillary electrophoresis[J]. J. Sep. Sci., 2015, 26(12-13):1203-1206.

HUANG H, XU Y, TANG C J, et al.. Facile and green synthesis of photoluminescent carbon nanoparticles for cellular imaging[J]. New J. Chem., 2014, 38(2):784-789.

QU S, WANG X, LU Q, et al.. A biocompatible fluorescent ink based on water-soluble luminescent carbon nanodots[J]. Angew. Chem., 2012, 51(49):12215-12218.

李晓峰,周明, 龚爱华, 等. 氮掺杂碳量子点的合成、表征及其在细胞成像中的应用[J]. 材料科学与工程学报, 2015, 33(1):41-45. LI X F, ZHOU M, GONG A H, et al.. Synthesis and characterization of nitrogen doped carbon quantum dots and its application in cell imaging[J]. J. Mater. Sci. Eng., 2015, 33(1):41-45. (in Chinese)

ZHANG X, MA Z, YANG Y, et al.. Influence of alkyl length on properties of piezofluorochromic aggregation induced emission compounds derived from 9,10-bis[(N-alkylphenothiazin-3-yl)vinyl] anthracene[J]. Tetrahedron., 2014, 70(4):924-929.

HUANG S, ZHU F, XIAO Q, et al.. A CdTe/CdS/ZnS core/shell/shell QDs-based "off-on" fluorescent biosensor for sensitive and specific determination of L-ascorbic acid[J]. RSC Adv., 2014, 4(87):46751-46761.

GAO Z, WANG L, SU R, et al.. A carbon dot-based "off-on" fluorescent probe for highly selective and sensitive detection of phytic acid[J]. Biosens. Bioelectron., 2015, 75(15):232-238

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红枣碳量子点“关-开”型荧光探针检测芦丁的研究

Synthesized of Carbon Quantum Dots from Red Dates and as A “Off-On” Fluorescence Probe for High Sensitive Detection of Rutin

DOI：10.3788/fgxb20183904.0600

摘要

Abstract

关键词

Keywords

references