核酸适体传感法检测中药材中黄曲霉毒素B1

曾云龙; 程圆昕; 易守军; 张敏; 何盼; 赵敏; 唐春然

doi:10.3788/fgxb20183904.0589

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核酸适体传感法检测中药材中黄曲霉毒素B1

发光学应用及交叉前沿 | 更新时间：2020-08-12

- 核酸适体传感法检测中药材中黄曲霉毒素B1
- A Novel Aptasensor Assay for Aflatoxin B1 in Traditional Chinese Medicine
- 发光学报 2018年39卷第4期页码：589-594
- 作者机构：
  
  1. 湖南科技大学化学化工学院理论有机和功能分子教育部重点实验室, 湖南湘潭 411201
  2. 湖南科技大学材料科学与工程学院, 湖南湘潭 411201
- 作者简介：
- 基金信息：
  
  国家自然科学基金（21475040）资助项目()
- DOI：10.3788/fgxb20183904.0589
  中图分类号： O611.4
- 纸质出版日期：2018-4-5，
  
  网络出版日期：2017-11-3，
  
  收稿日期：2017-8-15，
  
  修回日期：2017-10-17，
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曾云龙, 程圆昕, 易守军等. 核酸适体传感法检测中药材中黄曲霉毒素B1[J]. 发光学报, 2018,39(4): 589-594

ZENG Yun-long, CHENG Yuan-xin, YI Shou-jun etc. A Novel Aptasensor Assay for Aflatoxin B1 in Traditional Chinese Medicine[J]. Chinese Journal of Luminescence, 2018,39(4): 589-594
曾云龙, 程圆昕, 易守军等. 核酸适体传感法检测中药材中黄曲霉毒素B1[J]. 发光学报, 2018,39(4): 589-594 DOI： 10.3788/fgxb20183904.0589.

ZENG Yun-long, CHENG Yuan-xin, YI Shou-jun etc. A Novel Aptasensor Assay for Aflatoxin B1 in Traditional Chinese Medicine[J]. Chinese Journal of Luminescence, 2018,39(4): 589-594 DOI： 10.3788/fgxb20183904.0589.

摘要

以自组装方法，构建了金纳米粒子/核酸适体/CdTe量子点复合物荧光传感体系，金纳米粒子使复合物中量子点荧光猝灭。样品溶液中存在黄曲霉毒素B1（AFB1）时，复合物中核酸适体选择性地与AFB1结合，并释放出量子点，使体系的荧光得到恢复，其荧光强度随加入样品中AFB1的量增大而增大。AFB1浓度在0.005~2.00 ng/mL范围与体系荧光强度恢复呈良好线性关系，检测限为1.2 pg/mL。该法用于连翘、山楂和甘草等中药材中痕量AFB1测定，取得了满意结果。

Abstract

The nanocomposites of Au nanoparticles/aptamers/quantum dots were constructed by self-assembly. Fluorescence of CdTe quantum dots can be quenched by Au nanoparticles in the nanocomposites. The fluorescence of the system can recover when aflatoxin B1 (AFB1) is added to the assay solution

specific interactions between AFB1 and the aptamer cause release of the quantum dots. A linear fluorescence signal response to AFB1 concentration is obtained over a wide AFB1 concentration range of 0.005-2.00 ng/mL with a detection limit of 1.2 pg/mL. The proposed method has been employed in the determination of trace AFB1 in forsythia suspense

hawthorn and radix liquiritiae successfully.

关键词

核酸适体量子点测定中药材黄曲霉毒素B1

Keywords

aptasensorquantum dotsdeterminationtraditional chinese medicineaflatoxin B1

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