柯里拉京与DNA相互作用的光谱研究

申炳俊; 刘占伟; 刘荣娟; 张佳佳; 林晓倩; 田坚; 金丽虹

doi:10.3788/fgxb20173812.1681

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柯里拉京与DNA相互作用的光谱研究

发光学应用及交叉前沿 | 更新时间：2020-08-12

- 柯里拉京与DNA相互作用的光谱研究
- Study on Mechanism of Interactions Between DNA and Corilagin by Spectroscopy
- 发光学报 2017年38卷第12期页码：1681-1687
- 作者机构：
  
  1. 长春理工大学清洁能源技术研究所, 吉林长春 130022
  2. 长春理工大学生命科学技术学院, 吉林长春 130022
- 作者简介：
- 基金信息：
  
  国家自然科学基金（21153003）();吉林省教育厅项目（201574）();长春理工大学博士后基金（2014年，889）资助项目()
- DOI：10.3788/fgxb20173812.1681
  中图分类号： O657.3
- 纸质出版日期：2017-12-5，
  
  网络出版日期：2017-8-16，
  
  收稿日期：2017-4-24，
  
  修回日期：2017-7-24，
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申炳俊, 刘占伟, 刘荣娟等. 柯里拉京与DNA相互作用的光谱研究[J]. 发光学报, 2017,38(12): 1681-1687

SHEN Bing-jun, LIU Zhan-wei, LIU Rong-juan etc. Study on Mechanism of Interactions Between DNA and Corilagin by Spectroscopy[J]. Chinese Journal of Luminescence, 2017,38(12): 1681-1687
申炳俊, 刘占伟, 刘荣娟等. 柯里拉京与DNA相互作用的光谱研究[J]. 发光学报, 2017,38(12): 1681-1687 DOI： 10.3788/fgxb20173812.1681.

SHEN Bing-jun, LIU Zhan-wei, LIU Rong-juan etc. Study on Mechanism of Interactions Between DNA and Corilagin by Spectroscopy[J]. Chinese Journal of Luminescence, 2017,38(12): 1681-1687 DOI： 10.3788/fgxb20173812.1681.

摘要

在pH 7.4的生理条件下，以溴化乙锭（EB）作为荧光探针，利用荧光光谱、紫外-可见吸收光谱、共振散射光谱法结合盐效应和DNA熔点（

）实验研究了柯里拉京（Cor）与小牛胸腺DNA分子之间的相互作用机制。实验结果表明，Cor静态猝灭DNA-EB体系的荧光。Cor与DNA作用后，其特征吸收峰强度发生减色效应；与DNA作用导致Cor在480.5 nm处的共振散射峰增强，并在330.2 nm处出现新共振散射峰。盐效应对Cor与DNA分子相互作用的影响较小。与Cor作用引起DNA的

值升高5.5℃。由此推断，Cor与DNA相互作用的主要方式为嵌插，两者间形成了超分子体系。通过计算获得Cor与DNA间结合常数（

）为5.8210

L/mol（298 K）、2.4710

L/mol（310 K），它们之间的作用为熵驱动的自发、吸热过程，疏水作用力是主要的非共价作用方式。

Abstract

Under the physiological condition of pH 7.4

using ethidium bromide (EB) as the fluorescent probe

the interaction mechanism between coriolis (Cor) and calf thymus DNA was studied by UV-Vis absorption spectroscopy

resonance scattering spectroscopy combined with salt effect and DNA melting point. Cor can quench the fluorescence of EB-DNA system through a static process. The subtractive effect of the characteristic absorption peak occurrs after Cor interacts with DNA. The interaction leads to the enhancement of Cor resonance at 481 nm and a new resonance scattering peak at 330 nm. The salt effect has a little influence on the interaction between Cor and DNA. The interaction leads to the increase of the

of DNA by 5.5℃. It is deduced that the main way of the interaction between Cor and DNA is intercalation

and a supramolecular system is formed between them. The binding constant (

) between Cor and DNA is 5.8210

L/mol (298 K) and 2.4710

L/mol (310 K). The interaction between Cor and DNA is entropy-driven spontaneous

endothermic. Hydrophobic forces are the main non-covalent mode of action.

关键词

柯里拉京小牛胸腺DNA光谱学嵌插反应机理

Keywords

corilagincalf thymus DNAspectra methodintercalationreaction mechanism

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