双氰基荧光染料的合成、光学性质及其生物成像

李龙龙; 赵宁; 李冰; 彭丹; 周宁宁; 刘世新

doi:10.3788/fgxb20173812.1575

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双氰基荧光染料的合成、光学性质及其生物成像

材料合成及性能 | 更新时间：2020-08-12

- 双氰基荧光染料的合成、光学性质及其生物成像
- Synthesis, Optical Properties and Biological Imaging of A New Two-photon Fluorescence Dye with Dicyano-group
- 发光学报 2017年38卷第12期页码：1575-1581
- 作者机构：
  
  1. 山东省科学院新材料研究所山东省特种含硅新材料重点实验室,山东济南,250014
  2. 山东省计量科学研究院,山东济南,250014
  3. 山东省标准化研究院,山东济南,250014
- 作者简介：
- 基金信息：
  
  国家自然科学基金青年基金（51303097）资助项目()
- DOI：10.3788/fgxb20173812.1575
  中图分类号： O625.67
- 纸质出版日期：2017-12-5，
  
  网络出版日期：2017-8-28，
  
  收稿日期：2017-4-26，
  
  修回日期：2017-8-18，
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李龙龙, 赵宁, 李冰等. 双氰基荧光染料的合成、光学性质及其生物成像[J]. 发光学报, 2017,38(12): 1575-1581

LI Long-long, ZHAO Ning, LI Bing etc. Synthesis, Optical Properties and Biological Imaging of A New Two-photon Fluorescence Dye with Dicyano-group[J]. Chinese Journal of Luminescence, 2017,38(12): 1575-1581
李龙龙, 赵宁, 李冰等. 双氰基荧光染料的合成、光学性质及其生物成像[J]. 发光学报, 2017,38(12): 1575-1581 DOI： 10.3788/fgxb20173812.1575.

LI Long-long, ZHAO Ning, LI Bing etc. Synthesis, Optical Properties and Biological Imaging of A New Two-photon Fluorescence Dye with Dicyano-group[J]. Chinese Journal of Luminescence, 2017,38(12): 1575-1581 DOI： 10.3788/fgxb20173812.1575.

摘要

设计合成了一种A--D--A型的双光子荧光染料3，6-双（4-乙烯基苯腈）-9-乙基咔唑，测试了其在二氯甲烷（DCM）、乙酸乙酯（EA）、乙醇（EtOH）、乙腈（ACN）、二甲亚砜（DMSO）和磷酸缓冲盐溶液（PBS）等不同溶剂中的紫外吸收光谱、单光子及双光子荧光光谱。化合物3，6-双（4-乙烯基苯腈）-9-乙基咔唑在紫外吸收光谱中存在两个相似的特征吸收带并呈现出复杂的溶剂化效应，在DMSO中具有最大荧光量子产率（86.02%），其相应的活性吸收截面为12.56 GM。在双光子荧光成像方面，染料分子具有优良的细胞膜通透性并且在双光子荧光显微镜下呈现出明亮的绿色荧光，表现出较好的双光子荧光成像性能。这些数据表明，化合物3，6-双（4-乙烯基苯腈）-9-乙基咔唑可用作一种较为理想的双光子荧光标记染料。

Abstract

A novel two-photon fluorescence dye 4

4'-((1Z

1'Z)-(9-ethyl-9H-carbazole-3

6-diyl)bis(ethene-2

1-diyl))dibenzonitrile was synthesized

and the linear absorption and fluorescent spectra of the dye in different solvents were investigated. The results indicate that the dye has a intricate solvent effect existing in UV-Vis absorption spectra

and the quantum yields and two-photon active cross-sections in DMSO are 86.02%

12.56 GM

respectively. About the images

the dye exhibites excellent cell membrane permeability and optical properties. These data show that the compound is a promising candidate for two-photon fluorescence labels.

关键词

双光子荧光染料紫外吸收量子产率活性吸收截面细胞成像

Keywords

two-photon fluorescence dyeUV-Vis absorptionquantum yieldsactive cross-sectionsbiological imaging

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