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1. 南京航空航天大学 理学院, 江苏 南京 210016
2. 南京师范大学,江苏 南京,210046
纸质出版日期:2017-7-5,
收稿日期:2016-12-5,
修回日期:2017-2-4,
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葛学峰, 吴彦玮, 赵志敏. 荧光光谱法检测蜂蜜中腐霉利农药的含量[J]. 发光学报, 2017,38(7): 973-977
GE Xue-feng, WU Yan-wei, ZHAO Zhi-min. Deteramination of Procymidone Residues Content in Honey by Fluorescence Spectroscopy[J]. Chinese Journal of Luminescence, 2017,38(7): 973-977
葛学峰, 吴彦玮, 赵志敏. 荧光光谱法检测蜂蜜中腐霉利农药的含量[J]. 发光学报, 2017,38(7): 973-977 DOI: 10.3788/fgxb20173807.0973.
GE Xue-feng, WU Yan-wei, ZHAO Zhi-min. Deteramination of Procymidone Residues Content in Honey by Fluorescence Spectroscopy[J]. Chinese Journal of Luminescence, 2017,38(7): 973-977 DOI: 10.3788/fgxb20173807.0973.
为了实现农产品中农药残留含量快速分析,根据荧光光谱的基本原理研究了腐霉利的荧光特性,对腐霉利-蜂蜜混合体系进行了研究,分析荧光强度与蜂蜜中腐霉利含量之间的关系。采用稳态荧光光谱仪分别检测腐霉利溶液和蜂蜜溶液的荧光光谱,纯蜂蜜溶液荧光峰在342 nm,腐霉利溶液的荧光峰在390 nm,通过逐次向纯蜂蜜溶液中添加0.1 g/dL的腐霉利标准溶液,发现相对于纯蜂蜜,荧光峰位置发生红移,说明腐霉利与蜂蜜中某些物质发生了相互作用。采用Origin8.5数据分析软件对蜂蜜的荧光峰值处荧光峰强度与腐霉利含量线性拟合后,发现蜂蜜溶液中腐霉利的含量与对应的荧光强度有良好的线性关系,预测函数模型为I=188504.75
x
+384764.33,相关系数为0.99。经过相同实验条件下的实验验证,模型预测准确率在96.8%~100%之间。研究结果表明:荧光光谱法检测蜂蜜中腐霉利残留含量的方法是行之有效的,为其他农产品中腐霉利农药残留含量的检测提供了参考依据。
In order to offer the quick analysis of concentration of pesticides residues in agricultural products
a mixed procymidone-honey system was built and studied according to the principle of fluorescence spectrum. The relationship between the fluorescence intensity and the procymidone content in honey was also analyzed. With the aid of steady fluorescence spectrometer
the fluorescence spectra of procymidone solution and honey solution were detected respectively. The fluorescence emission peaks of pure honey and procymidone are found at about 342 nm and 390 nm. The fluorescence peak position has a red shift (from 342 nm to 390 nm) with respect to an addition of procymidone (0.1 g/dL) into honey
indicating the interaction between procymindone and some substances in honey. By using software program of Origin8.5 which is used to process and analyze data
the linear fitting is done of the fluorescence peak intensity of the honey and the content of procymindone. The results show that the prediction models between the fluorescence intensity and procymidone content in honey has a good linear relationship. The function model is I=188504.75
x
+384764.33
and the correlation coefficient is 0.99. The experimental results under the same conditions show that the accuracy of the function model is 96.8%-100%. The results can be concluded that the determination of procymidone is effective and reliable for the concentration testing of procymidone residues in other agricultural products.
荧光光谱蜂蜜腐霉利含量检测
fluorescence spectrumhoneyprocymidoneconcentration detection
JIANG X, LI D, XIA X, et al.. Immunosensors for detection of pesticide residues[J]. Biosens. Bioelectron., 2008, 23(11):1577-1587.
张国军. 八种常用杀菌剂"三致"作用及生殖毒性研究进展[J]. 中国预防医学杂志, 2007, 8(3):320-320. ZHANG G J. Research progress of "three effects" and reproductive toxicity of eight commonly used fungicides[J]. Chin. Prevent. Med., 2007, 8(3):320-320. (in Chinese)
任刚, 廖卫波, 胡志成, 等. 人参提取物中腐霉利残留的HPLC分析[J]. 中国实验方剂学杂志, 2013, 19(23):107-110. REN G, LIAO W B, HU Z C, et al.. Analysis of procymidone residues in ginseng extract by HPLC[J]. Chin. J. Experiment. Trad. Med. Formulae, 2013, 19(23):107-110. (in Chinese)
祁超, 张浩, 潘洪玉, 等. 腐霉利的高效液相色谱分析[J]. 农药, 1998, 37(8):18-19. QI C, ZHANG H, PAN H Y, et al.. Determination of procymidone by RP-HPLC[J]. Pesticides, 1998, 37(8):18-19. (in Chinese)
FULG?NCIO A C D C, SACZK A A, OLIVEIRA M F D, et al.. New voltammetry-based analytical method for indirect determination of procymidone in brazilian apples[J]. Food Anal. Methods, 2014, 7(1):31-38.
SOARES A A, AMARAL E H, FERREIRA D S, et al.. In-house method validation and occurrence of alpha-, beta-endosul fan, endosulfan sulphat, lambda-cyhalothrin, procymidone and trifluralin residues in strawberry[J]. Food Sci. Technol., 2013, 33(4):765-775.
ZHANG C P, ZHAP H, WU M, et al.. Simultaneous determination of procymidone, pyridaben and beta-cypermethrin residues in tea solution by GC-ECD[J]. J. Chromatograph. Sci., 2012, 50(10):940-944.
陈琼, 喻芳, 张惠, 等. QuEChERS法处理结合液质法测茶叶中多种农残[J]. 广州化工, 2015, 43(16):143-146. CHEN Q, YU F, ZHANG H, et al.. The improved QuECh method to deal with a variety of pesticide residues in tea by liquid chromatography-mass spectrometry method[J]. Guangzhou Chem. Industry, 2015, 43(16):143-146. (in Chinese)
张群, 吴南村, 张月, 等. 气相色谱-串联质谱法测定韭菜中的三唑酮和腐霉利残留[J]. 农药学学报, 2016, 18(3):397-401. ZHANG Q, WU N C, ZHANG Y, LIU C H, et al.. Determination of triadimefon and procymidone residues in allium tuberosum by gas chromatography-tandem mass spectrometry[J]. Chin. J. Pesticide Sci., 2016, 18(3):397-401. (in Chinese)
解魁, 张伟, 杨丽, 等. 气相色谱-质谱联用法测定人参中五氯苯胺和腐霉利的残留[J]. 中国卫生检验杂志, 2015, 25(21):624-3628 XIE K, ZHANG W, YANG L, et al.. Determination of pentachloroaniline and procymidone residues in ginseng by gas chromatography-mass spectrometry[J]. Chin. J. Health Lab. Technol., 2015, 25(21):624-3628. (in Chinese)
宋博才. 气相色谱法测定草莓的农残含量[J]. 安徽农业科学, 2016, 44(10):133-135. SONG B C. Determination of pesticide residues in strawberries by gas chromatography[J]. J. Anhui Agric. Sci., 2016, 44(10):133-135. (in Chinese)
邱伟芬, 张昌娟, 文良, 等. 气相色谱-质谱联用结合QuEChERS法快速筛查大米中多种农药残留[J]. 食品科学, 2015, 36(12):221-225. QIU W F, ZHANG C J, WEN L, et.al.. Rapid determination of pesticide multi-residues in rice by QuEChERS and GC-MS[J]. Food Sci., 2015, 36(12):221-225. (in Chinese)
TAIRA S, TOKAI M, KANEKO D, et al.. Mass spectrometry imaging analysis of location of procymidone in cucumber samples[J]. J. Agric. Food Chem., 2015, 63(27):181-184.
ARAJO E A, LARA M C R, REIS M R D, et al.. Determination of haloxyfop-methyl, linuron, and procymidone pesticides in carrot using SLE-LTP extraction and GC-MS[J]. Food Anal. Methods, 2016, 9(5):1-9.
KORBA K, PELIT L, PELIT F, et al.. Preparation and characterization of sodium dodecyl sulfate doped polypyrrole solid phase micro extraction fiber and its application to endocrine disruptor pesticide analysis[J]. J. Chromatograph. B, 2013, 929:90-96.
朱学良, 戚向阳, 刘传菊, 等. 基质固相分散-气相色谱法测定桃中3种残留杀真菌剂[J]. 理化检验(化学分册), 2009, 45(1):46-48. ZHU X L, QI X Y, LIU C J, et al..GC determination of 3 residual fungicides in peach with matrix solid phase dispersion of sample[J]. Phys. Testing Chem. Anal.(Part B:Chem. Anal.), 2009, 45(1):46-48. (in Chinese)
许金钩, 王尊本. 荧光分析法[M]. 北京:科学出版社, 2007. XU J G, WANG Z B. Fluorescence Analysis[M]. Beijing:Science Press, 2007. (in Chinese)
徐寿昌. 有机化学[M]. 北京:高等教育出版社, 1993. XU S C. Organic Chemistry[M]. Beijing:Higher Education Press, 1993. (in Chinese)
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