基于置换法的硫离子荧光传感器的研究及其在生物细胞成像中的应用

程晓红; 钟志成; 李望南

doi:10.3788/fgxb20173806.0768

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基于置换法的硫离子荧光传感器的研究及其在生物细胞成像中的应用

器件制备及器件物理 | 更新时间：2020-08-12

- 基于置换法的硫离子荧光传感器的研究及其在生物细胞成像中的应用
- Coumarin-derived Fluorescent Chemosensor for Sulfide Anion:Displacement Approach and Bioimaging Application
- 发光学报 2017年38卷第6期页码：768-779
- 作者机构：
  
  湖北文理学院低维光电材料与器件湖北省重点实验室,湖北襄阳,441053
- 作者简介：
- 基金信息：
  
  国家自然科学基金（21502047）();湖北省光电子协同创新中心基金资助项目()
- DOI：10.3788/fgxb20173806.0768
  中图分类号： O622.4
- 纸质出版日期：2017-6-5，
  
  收稿日期：2017-1-1，
  
  修回日期：2017-3-15，
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程晓红, 钟志成, 李望南. 基于置换法的硫离子荧光传感器的研究及其在生物细胞成像中的应用[J]. 发光学报, 2017,38(6): 768-779

CHENG Xiao-hong, ZHONG Zhi-cheng, LI Wang-nan. Coumarin-derived Fluorescent Chemosensor for Sulfide Anion:Displacement Approach and Bioimaging Application[J]. Chinese Journal of Luminescence, 2017,38(6): 768-779
程晓红, 钟志成, 李望南. 基于置换法的硫离子荧光传感器的研究及其在生物细胞成像中的应用[J]. 发光学报, 2017,38(6): 768-779 DOI： 10.3788/fgxb20173806.0768.

CHENG Xiao-hong, ZHONG Zhi-cheng, LI Wang-nan. Coumarin-derived Fluorescent Chemosensor for Sulfide Anion:Displacement Approach and Bioimaging Application[J]. Chinese Journal of Luminescence, 2017,38(6): 768-779 DOI： 10.3788/fgxb20173806.0768.

摘要

报道了一种基于香豆素衍生物与铜离子的络合物（C1-Cu），可通过间接的方法检测硫离子。络合物C1-Cu的溶液中加入硫离子后，表现出明显的荧光增强响应，检测灵敏度高，检出限低达90 nmol/L；响应速度快，可实现硫离子的实时检测；荧光强度变化与硫离子浓度呈现良好的线性关系，能准确地定量分析硫离子浓度。上述络合物C1-Cu对硫离子的检测具有超高的选择性，即使在其他阴离子存在下也能高效地识别出硫离子；得益于香豆素良好的水溶性和生物相容性，上述络合物C1-Cu可实现生物细胞中硫离子的荧光成像。除荧光光谱响应之外，该体系对硫离子亦有紫外-可见光谱响应，无需借助任何仪器便可实现对硫离子方便、快捷的"裸眼"检测。

Abstract

We reported herein an ensemble C1-Cu

as a new displacement-based fluorescent probe for sulfide anion detection. Upon the addition of S

it displayed marked fluorescence enhancement under aqueous conditions and the detection limit was determined to be as low as 90 nmol/L. In addition to its high selectivity for sulfide anion rather than other common anions

C1 was successfully applied to the detection of sulfide anion in HeLa cells with Turn-On fluorescent methods.

关键词

置换法荧光增强硫离子水溶性生物成像

Keywords

displacement approachfluorescence enhancementsulfide aniongood solubilitybioimaging application

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