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1. 东南大学生物科学与医学工程学院 生物电子学国家重点实验室, 江苏 南京 210096
2. 黔南民族医学高等专科学校, 贵州 都匀 558000
纸质出版日期:2017-3-5,
收稿日期:2016-9-19,
修回日期:2016-10-15,
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胡先运, 孟铁宏, 张汝国等. 基于InP@ZnS QDs/Dured纳米荧光探针的DNA检测[J]. 发光学报, 2017,38(3): 288-295
HU Xian-yun, MENG Tie-hong, ZHANG Ru-guo etc. InP@ZnS QDs/Dured Fluorescent Nanoprobe for The Detection of DNA[J]. Chinese Journal of Luminescence, 2017,38(3): 288-295
胡先运, 孟铁宏, 张汝国等. 基于InP@ZnS QDs/Dured纳米荧光探针的DNA检测[J]. 发光学报, 2017,38(3): 288-295 DOI: 10.3788/fgxb20173803.0288.
HU Xian-yun, MENG Tie-hong, ZHANG Ru-guo etc. InP@ZnS QDs/Dured Fluorescent Nanoprobe for The Detection of DNA[J]. Chinese Journal of Luminescence, 2017,38(3): 288-295 DOI: 10.3788/fgxb20173803.0288.
利用巯基丙酸包覆的InP@ZnS量子点(QDs)与Dured构建了一种检测DNA的荧光探针。在该探针中,以环境友好型带负电的InP@ZnS量子点为荧光团,与带正电的Dured通过静电结合,构建了InP@ZnS QDs/Dured纳米荧光探针。通过荧光共振能量转移(FRET)机理,量子点荧光被猝灭;当DNA存在时,Dured与DNA的特异性结合使Dured从InP@ZnS QDs表面脱附,FRET过程被打断,InP@ZnS QDs荧光恢复,以荧光关-开方式检测DNA。该探针检测DNA的线性范围为2.0~275.0 ngL
-1
,检测限为1.0 ngL
-1
,并可用于模拟生物生理条件下的DNA检测。
The fluorescent nanoprobe for the detection of DNA was established by utilizing mercaptopropionic acid coated InP@ZnS QDs and Dured. In this nanoprobe. The InP@ZnS QDs/Dured fluorescent nanoprobes were structured by electrostatic interactions between environment friendly
negatively charged InP@ZnS quantum dots and positively charged Dured
and then the fluorescence of InP@ZnS QDs/Dured was quenched through fluorescence resonance energy transfer (FRET). At presence of DNA
DNA and Dured were specific binding
which enable Dured removing from the surface of InP@ZnS QDs and achieving fluorescence recovery of InP@ZnS QDs. Thus
the detection of DNA was realized. The linear range of the InP@ZnS QDs/Dured fluorescent nanoprobes for DNA detection and the detection limit were 2.0-275.0 ngL
-1
and 1.0 ngL
-1
respectively. The InP@ZnS QDs/Dured fluorescent nanoprobes can be also used in rapid detection of DNA under simulated physiological conditions.
InP@ZnS量子点荧光探针DNA荧光共振能量转移
InP@ZnS quantum dotsfluorescent probeDNAFRET
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