浏览全部资源
扫码关注微信
1. 长春理工大学 清洁能源技术研究所,吉林 长春,130022
2. 长春理工大学 生命科学技术学院,吉林 长春,130022
纸质出版日期:2016-10-5,
收稿日期:2016-4-18,
修回日期:2016-8-4,
扫 描 看 全 文
申炳俊, 金丽虹, 张佳佳等. 对-香豆酸与人血清白蛋白相互作用的荧光和表面增强拉曼光谱研究[J]. 发光学报, 2016,37(10): 1259-1266
SHEN Bing-jun, JIN Li-hong, ZHANG Jia-jia etc. Investigation on The Interaction between <em>p</em>-Coumaric Acid and Human Serum Albumin by Fluorescence and Surface Enhanced Raman Spectra[J]. Chinese Journal of Luminescence, 2016,37(10): 1259-1266
申炳俊, 金丽虹, 张佳佳等. 对-香豆酸与人血清白蛋白相互作用的荧光和表面增强拉曼光谱研究[J]. 发光学报, 2016,37(10): 1259-1266 DOI: 10.3788/fgxb20163710.1259.
SHEN Bing-jun, JIN Li-hong, ZHANG Jia-jia etc. Investigation on The Interaction between <em>p</em>-Coumaric Acid and Human Serum Albumin by Fluorescence and Surface Enhanced Raman Spectra[J]. Chinese Journal of Luminescence, 2016,37(10): 1259-1266 DOI: 10.3788/fgxb20163710.1259.
在模拟生理条件下,应用荧光光谱和表面增强拉曼光谱法对对-香豆酸(
p
-CA)与人血清白蛋白 (HSA) 的结合机理进行研究。结果表明,
p
-CA对HSA的荧光猝灭机制为静态猝灭,并伴有非辐射能量转移。荧光光谱显示,在298,304,310 K下,
p
-CA与HSA的结合常数(
K
A
)分别为3.4110
4
,2.0910
4
,1.3810
4
L/mol,结合位点数(
n
)近似为1。表面增强拉曼光谱研究揭示,
p
-CA的酚基与HSA有效结合。标记竞争实验指出,
p
-CA在HSA上的结合位点主要在SiteⅠ。反应过程热力学参数表明,二者间的作用主要为静电引力,且根据Frster能量转移理论求得
p
-CA与HSA间的距离为 5.11 nm。同步荧光光谱显示,
p
-CA的结合没有导致HSA构象发生明显变化。
Under simulated physiological conditions
the binding mechanism between
p
-Coumaric acid (
p
-CA) and human serum albumin (HSA) was investigated by fluorescence spectrum and surface enhanced Raman scattering (SERS). The results show that the effect between
p
-CA and HSA is a static fluorescence quenching with Frster's non-radioactive energy transformation. At 298
304
310 K
the binding constants (
K
A
) between
p
-CA and HSA are 3.4110
4
2.0910
4
1.3810
4
L/mol
the binding site (
n
) value is approximate to 1. SERS reveals that the phenolic group of
p
-CA combines with HSA. Thermodynamic data indicate that the interaction between
p
-CA and HSA is mainly electrostatic attraction. Marker competition experiments point out that the primary binding site for
p
-CA is located at site Ⅰ in HSA. According to Frster energy transfer theory
the binding distance between
p
-CA and HSA is 5.11 nm. Synchronous fluorescence spectra show that the conformation of HSA does not changed apparently with the addition of
p
-CA.
荧光光谱表面增强拉曼光谱对-香豆酸人血清白蛋白反应机理
fluorescence spectrumsurface enhanced Raman scatteringp-Coumaric acidhuman serum albuminreaction mechanism
YAN Y, XU J, CHEN G Z. Protein conformation in solution by three-dimensional fluorescence spectrometry [J]. Sci. China Ser. B, 1996, 39(5):527-535.
CHENG J C, DAI F, ZHOU B, et al.. Antioxidant activity of hydroxy cinnamic acid derivatives in human low density lipoprotein: mechanism and structure-activity relationship [J]. Food Chem., 2007, 104(1):132-139.
BISWICK T, PARK D H, SHUL Y G, et al.. p-Coumaric acid-zinc basic salt nanohybrid for controlled release and sustained antioxidant activity [J]. J. Phys. Chem. Solids, 2010, 71(4):647-649.
AAHRO J R, P STANELY M P. Preventive effects of p-Coumaric acid on cardiac hypertrophy and alterations in electrocardiogram, lipids, and lipoproteins in experimentally induced myocardial infarcted rats [J]. Food Chem. Toxicol., 2013, 60(10):348-354.
AADEL-WAHAB M H, El-MAHDY M A, ABD-ELLAH M F, et al.. Influence of p-Coumaric acid on doxorubicin-induced oxidative stress in rat's heart [J]. Pharmacol. Res., 2003, 48(5):461-465.
BODINI S F, MANFREDINI S, EPP M, et al.. Quorum sensing inhibition activity of garlic extract and p-Coumaric acid [J]. Lett. Appl. Microbial., 2009, 49(5):551-555.
陆从文,兰秀风,张林,等. 依巴斯汀与牛血清蛋白相互作用的荧光光谱研究 [J]. 光子学报, 2015, 44(10):1030004. LU C W, LAN X F, ZHANG L, et al.. Interaction between ebastine and bovine serum albumin by fluorescence spectroscopy [J]. Acta Photon. Sinica, 2015, 44(10):1030004. (in Chinese)
刘保生,韩荣,李志云,等. 硝基羟乙唑与溶菌酶反应机制的荧光光谱研究 [J]. 发光学报, 2015, 36(12):1458-1463. LIU B S, HUA R, LI Z Y, et al.. Investigation of the interaction mechanism between lysozyme and trichazol using fluorescence spectroscopic method [J]. Chin. J. Lumin., 2015, 36(12):1458-1463. (in Chinese)
JIANG H, CHEN R R, PU H L. Study on the interaction between tabersonine and human serum albumin by optical spectroscopy and molecular modeling methods [J]. J. Lumin., 2012, 132(3):592-599.
ZHANG W, WANG Y P, BAI X Y, et al.. Based on SERS conformational studies of ginsenoside Rb1 and its metabolites before and after combined with human serum albumin [J]. Spectrochim. Acta Part A: Mol. Biomol. Spect., 2015, 137:116-121
张巍,赵雨,白雪媛,等. 对黄芩素和黄芩苷与人血清白蛋白相互作用的光谱研究 [J]. 高等学校化学学报, 2010, 31(9):1834-1840. ZHANG W, ZHAO X, BAI X Y, et al.. Studies on the interaction for baicalein and baicalin with human serum albumin by spectroscopic methods [J]. Chem. J. Chi. Univ., 2010, 31(9):1834-1840. (in Chinese)
WANG W N, ZHANG W, DUAN Y K, et al.. Investigation of the binding sites and orientation of caffeine on human serum albumin by surface-enhanced Raman scattering and molecular docking [J]. Spectrochim. Acta Part A: Mol. Biomol. Spect., 2013, 115(11):57-63.
WANG Q, HE J W, YAN J, et al.. Spectroscopy and docking simulations of the interaction between lochnericine and bovine serum albumin [J]. Luminescence, 2015, 30(2):240-246.
SUNDARAGANESAN N, DOMINIC J B, SETTU K. Vibrational spectra and assignments of 5-amino-2-chlorobenzoic acid by ab initio Hartree-Fock and density functional methods [J]. Spectrochim. Acta Part A: Mol. Biomol. Spect., 2007, 66(2):381-388.
LEE P C, MEISEL D. Adsorption and surface-enhanced Raman of dyes on silver and gold sols [J]. J. Phys. Chem., 1982, 86(17):3391-3395.
申炳俊,金丽虹,田坚. 荧光与紫外光谱法研究柚皮素与人血清白蛋白的结合模式 [J]. 分析试验室, 2016, 35(1):41-46. SHEN B J, JIN L H, TIAN J. Study of naringenin and its interaction with HSA by fluorescence and UV spectroscopies [J]. Chin. J. Anal. Lab., 2016, 35(1):41-46. (in Chinese)
PARAMAGURU G, KATHIRAVAN A, SELVARAJ S, et al., Interaction of anthraquinone dyes with lysozyme: evidences from spectroscopic and docking studies [J]. J. Hazard. Mater., 2010, 175(1-3):985-991.
杜传荣,逯东伟,石康,等. 三种肉桂酰胺衍生物的制备及其与人血清白蛋白的结合 [J]. 发光学报, 2015, 36(11):1342-1352. DU C R, LU D W, SHI K, et al.. Synthesis of three cinnamamide derivatives and their interaction mechanism with human serum albumin [J]. Chin. J. Lumin., 2015, 36(11):1342-1352. (in Chinese)
郭清莲,潘凌立,杨立云,等. 吡虫啉与人血清白蛋白相互作用热力学行为 [J]. 物理化学学报, 2016, 32(1):274-282. GUO Q L, PAN L L, YANG L Y, et al.. Thermodynamics of the interaction of imidacloprid with human serum albumin [J]. Acta Phys. -Chim. Sinica, 2016, 32(1):274-282. (in Chinese)
ROSS P D, SUBRAMANIAN S. Thermodynamic of protein association reactions: forces contributing to stability [J]. Biochemistry, 1981, 20(11):3096-3102.
WANG N, YE L, YAN F F, et al.. Spectroscopic studies on the interaction of azelnidipine with bovine serum albumin [J]. Int. J. Pharm., 2008, 351(1-2):55-60.
PALAFOX M A. Scaling factors for the prediction of vibrational spectra. Ⅰ. benzene molecule [J]. Int. J. Quant. Chem., 2000, 77(3):661-684.
0
浏览量
70
下载量
2
CSCD
关联资源
相关文章
相关作者
相关机构