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河北大学化学与环境科学学院 河北省分析科学技术重点实验室,河北 保定,071002
纸质出版日期:2016-7-5,
收稿日期:2016-1-28,
修回日期:2016-4-13,
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刘保生, 李彤彤, 张秋菊等. 不同光谱法用于药物与蛋白相互作用的比较研究[J]. 发光学报, 2016,37(7): 866-872
LIU Bao-sheng, LI Tong-tong, ZHANG Qiu-ju etc. Multi-spectrophotometric Methods for The Investigation of The Interactions of Bovine Serum Albumin and Drugs[J]. Chinese Journal of Luminescence, 2016,37(7): 866-872
刘保生, 李彤彤, 张秋菊等. 不同光谱法用于药物与蛋白相互作用的比较研究[J]. 发光学报, 2016,37(7): 866-872 DOI: 10.3788/fgxb20163707.0866.
LIU Bao-sheng, LI Tong-tong, ZHANG Qiu-ju etc. Multi-spectrophotometric Methods for The Investigation of The Interactions of Bovine Serum Albumin and Drugs[J]. Chinese Journal of Luminescence, 2016,37(7): 866-872 DOI: 10.3788/fgxb20163707.0866.
在模拟人体生理环境下
利用荧光猝灭法(FQS)、同步荧光法(SYS)、共振光散射法(RLS)及紫外吸收光谱法(UV)分别研究了298 K下牛血清白蛋白与硫酸粘杆菌素、硫酸头孢匹罗、头孢匹胺钠3种药物间的相互作用机理。结果表明:对于相同的体系
利用4种方法计算得到的结合常数在同一数量级
猝灭方式均为生成新物质的静态猝灭
药物与蛋白作用时均以1:1的比例结合
Hill系数近似。但对4种方法所得实验数据的综合比较表明
FQS、SYS更适合用于研究蛋白与药物的反应机理。
The interactions between colistin sulfate(CLS)
cefpirome sulfate(CFS)
cefpiramide sodium(CFMS) and bovine serum albumin(BSA) were investigated by fluorescence quenching spectroscopy (FQS)
synchronous fluorescence spectroscopy (SYS)
resonance light scattering (RLS) and UV absorption spectroscopy at 298 K with the simulated physiological condition of the body. The results show that the binding constants of BSA-CLS
BSA-CFS and BSA-CFMS obtained from four methods are at the same order of magnitude
and the quenching mechanism is a static quenching process. The number of binding site (
n
) in the three system is approximately equal to 1
and the values of Hill's coefficients in the three system are approximately consistent. The comparison to the experimental data for four methods indicates that FQS
SYS seem to be more suitable for the studying of the reaction mechanism of protein and drug.
牛血清白蛋白荧光猝灭法同步荧光法共振光散射法紫外吸收光谱法
bovine serum albuminfluorescence quenching spectroscopysynchronous fluorescence spectroscopyresonance light scattering spectroscopyUV-Vis absorption spectroscopy
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