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1. 苏州大学 药学院,江苏 苏州,215123
2. 苏州大学 第一附属医院,江苏 苏州,215123
3. 重庆科技学院 化学化工学院 重庆,401331
纸质出版日期:2016-3-5,
收稿日期:2015-10-16,
修回日期:2015-12-21,
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陈哲, 豆兴茹, 葛芝莉等. 利用聚丙烯酸钠-Nafion混合膜增强三联吡啶钌掺杂SiO<sub>2</sub>纳米粒的电化学发光信号及其生物标记[J]. 发光学报, 2016,37(3): 310-314
CHEN Zhe, DOU Xing-ru, GE Zhi-li etc. Electrochemiluminescence Signal Enhancement of Ru(bpy)<sub>3</sub><sup>2+</sup>-doped SiO<sub>2</sub> Nanoparticles Based on Sodium Polyacrylate-Nafion Mixing Membrane and Their Bio-labeling[J]. Chinese Journal of Luminescence, 2016,37(3): 310-314
陈哲, 豆兴茹, 葛芝莉等. 利用聚丙烯酸钠-Nafion混合膜增强三联吡啶钌掺杂SiO<sub>2</sub>纳米粒的电化学发光信号及其生物标记[J]. 发光学报, 2016,37(3): 310-314 DOI: 10.3788/fgxb20163703.0310.
CHEN Zhe, DOU Xing-ru, GE Zhi-li etc. Electrochemiluminescence Signal Enhancement of Ru(bpy)<sub>3</sub><sup>2+</sup>-doped SiO<sub>2</sub> Nanoparticles Based on Sodium Polyacrylate-Nafion Mixing Membrane and Their Bio-labeling[J]. Chinese Journal of Luminescence, 2016,37(3): 310-314 DOI: 10.3788/fgxb20163703.0310.
对于三联吡啶钌(Ru(bpy)
3
2+
)掺杂的SiO
2
纳米粒
以聚电解质聚丙烯酸钠和成膜物质Nafion的混合液对纳米粒进行包覆
制备了聚丙烯酸钠和Nafion混合膜包覆的电化学发光(ECL)纳米粒。结果表明:混合膜包覆的纳米粒
相对于Nafion膜的ECL信号增强了13倍。同时
混合膜表面可交换阳离子显著增加
能够通过离子交换固载大量的Ru(bpy)
3
2+
纳米粒的ECL信号可进一步增强约3倍。混合膜还具有另外一个显著的优势
即通过混合膜的疏水相互作用可以方便地标记生物大分子
标记抗体仍然具有良好的免疫活性。
Ru(bpy)
3
2+
-doped SiO
2
nanoparticles were covered with the mixture of polyelectrolyte sodium polyacrylate and membrane-forming substance Nafion
and then the electrochemiluminescence (ECL) nanoparticles were acquired. The results show that ECL intensity of the polyacrylate-Nafion mixing membrane covered nanoparticles is enhanced 13 times
compared with the Nafion membrane covered nanoparticles. At the same time
there is an obvious increase of the exchangeable cation on the surface of the mixing membrane
and more Ru(bpy)
3
2+
can be immobilized on the surface of the nanoparticles based on ion exchanging
and the corresponding ECL intensity can be enhanced about 3 times. Another significant advantage of the mixing membrane is that the as-prepared nanoparticles can label bio-macromolecule more easily based on the hydrophobic interaction. The results show that the labelled antibody still has good immune activity.
信号增强电化学发光纳米粒聚丙烯酸钠Nafion混合膜生物标记
signal enhancementelectrochemiluminescence nanoparticlessodium polyacrylateNafion-mixing membranebio-labelling
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QIAN J, ZHOU Z X, CAO X D, et al.. Electrochemiluminescence immunosensor for ultrasensitive detection of biomarker using Ru(bpy)32+-encapsulated silica nanosphere labels [J]. Anal. Chim. Acta, 2010, 665(1):32-38.
YANG X, YUAN R, CHAI Y Q, et al.. Ru(bpy)32+-doped silica nanoparticles labeling for a sandwich-type electrochemiluminescence immunosensor [J]. Biosens. Bioelectron., 2010, 25(7):1851-1855.
GE Z L, SONG T M, CHEN Z, et al.. Polyelectrolyte-based electrochemiluminescence enhancement for Ru(bpy)32+ loaded by SiO2 nanoparticle carrier and its high sensitive immunoassay [J]. Anal. Chim. Acta, 2015, 862:24-32.
郭武润,豆兴茹,葛芝莉,等. 三联吡啶钌超分子包合物及其SiO2复合纳米粒的电化学发光信号放大 [J]. 发光学报, 2014, 35(5):558-564. GUO W R, DOU X R, GE Z L, et al.. Electrochemiluminescence signal amplification of Ru(bpy)32+ supramolecular inclusion and its composite nanoparticles [J]. Chin. J. Lumin., 2014, 35(5):558-564. (in Chinese)
RUBINSTEIN I, BARD A J. Polymer films on electrodes. 4. Nafion-coated electrodes and electrogenerated chemiluminescence of surface-attached tris (2, 2'-bipyridine) ruthenium2+ [J]. J. Am. Chem. Soc., 1980, 102(21):6641-6642.
RUBINSTEIN I, RISHPON J, GOTTESFELD S. An AC-impedance study of electrochemical processes at nafion-coated electrodes [J]. J. Electrochem. Soc., 1986, 133(4):729-734.
WANG J, GOLDEN T. Permselectivity and ion-exchange properties of Eastman-AQ polymers on glassy carbon electrodes [J]. Anal. Chem., 1989, 61(13):1397-1400.
LIU H Y, YING T L, SUN K, et al.. Reagentless amperometric biosensors highly sensitive to hydrogen peroxide, glucose and lactose based on N-methyl phenazine methosulfate incorporated in a Nafion film as an electron transfer mediator between horseradish peroxidase and an electrode [J]. Anal. Chim. Acta, 1997, 344(3):187-199.
CHETCUTI A F, WONG D K Y, STUART M C. An indirect perfluorosulfonated ionomer-coated electrochemical immunosensor for the detection of the protein human chorionic gonadotrophin [J]. Anal. Chem., 1999, 71(18):4088-4094.
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