盐酸吡格列酮与BSA反应机理的荧光猝灭法、同步荧光法比较研究

李改霞; 刘保生; 李志云; 付家赫; 王天成

doi:10.3788/fgxb20153606.0705

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盐酸吡格列酮与BSA反应机理的荧光猝灭法、同步荧光法比较研究

发光学应用及交叉前沿 | 更新时间：2020-08-12

- 盐酸吡格列酮与BSA反应机理的荧光猝灭法、同步荧光法比较研究
- Comparative Studies on The Reaction Mechanism of Pioglitazone Hydrochloride with Bovine Serum Albumin by Fluorescence Spectroscopy and Synchronous Fluorescence Spectroscopy
- 发光学报 2015年36卷第6期页码：705-710
- 作者机构：
  
  河北大学化学与环境科学学院河北省分析科学技术重点实验室,河北保定,071002
- 作者简介：
- 基金信息：
  
  国家自然科学基金(21375032)();河北大学2014年大学生创新创业训练项目(2014089)资助()
- DOI：10.3788/fgxb20153606.0705
  中图分类号： O657.3
- 纸质出版日期：2015-6-3，
  
  收稿日期：2015-3-27，
  
  修回日期：2015-4-22，
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李改霞, 刘保生, 李志云等. 盐酸吡格列酮与BSA反应机理的荧光猝灭法、同步荧光法比较研究[J]. 发光学报, 2015,36(6): 705-710

LI Gai-xia, LIU Bao-sheng, LI Zhi-yun etc. Comparative Studies on The Reaction Mechanism of Pioglitazone Hydrochloride with Bovine Serum Albumin by Fluorescence Spectroscopy and Synchronous Fluorescence Spectroscopy[J]. Chinese Journal of Luminescence, 2015,36(6): 705-710
李改霞, 刘保生, 李志云等. 盐酸吡格列酮与BSA反应机理的荧光猝灭法、同步荧光法比较研究[J]. 发光学报, 2015,36(6): 705-710 DOI： 10.3788/fgxb20153606.0705.

LI Gai-xia, LIU Bao-sheng, LI Zhi-yun etc. Comparative Studies on The Reaction Mechanism of Pioglitazone Hydrochloride with Bovine Serum Albumin by Fluorescence Spectroscopy and Synchronous Fluorescence Spectroscopy[J]. Chinese Journal of Luminescence, 2015,36(6): 705-710 DOI： 10.3788/fgxb20153606.0705.

摘要

利用荧光猝灭法和同步荧光法进行了盐酸吡格列酮(PGH)与牛血清白蛋白(BSA)之间反应机理的比较研究。结果表明:两种方法在研究PGH-BSA体系的猝灭类型、结合常数、结合位置、作用力类型、协同性及能量转移距离等方面

得到了相同的结果

表明同步荧光光谱法可以用于药物与蛋白反应机理的研究。

Abstract

The reaction mechanism between pioglitazone hydrochloride (PGH) and bovine serum albumin (BSA) was studied by fluorescence quenching method and synchronous fluorescence method under different temperatures. The results show that the conclusions of two methods are identical on the quenching mechanism

type of interaction force

binding constants

binding location

synergistic effect

energy-transfer parameters of pioglitazone hydrochloride with bovine serum albumin

etc

. These indicate that the synchronous fluorescence spectroscopy can be used to replace the traditional fluorescence spectroscopy to study the reaction mechanism of drugs with protein.

关键词

荧光猝灭法同步荧光法盐酸吡格列酮牛血清白蛋白反应机理

Keywords

fluorescence spectroscopysynchronous fluorescence spectroscopypioglitazone hydrochloridebovine serum albuminreaction mechanism

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