基于FRET机理的荧光探针的合成及其对汞离子的识别

王海菊; 张文珠; 何成; 周硼

doi:10.3788/fgxb20123309.1030

您当前的位置：

首页 >

文章列表页 >

基于FRET机理的荧光探针的合成及其对汞离子的识别

光谱分析 | 更新时间：2020-08-12

- 基于FRET机理的荧光探针的合成及其对汞离子的识别
- Synthesis of Fluorescent Probe Based on FRET and Its Appalication in The Determination for Hg²⁺
- 发光学报 2012年33卷第9期页码：1030-1036
- 作者机构：
  
  大连理工大学化学学院精细化工国家重点实验室,辽宁大连,116021
- 作者简介：
- 基金信息：
  
  国家自然科学基金(21171029)资助项目()
- DOI：10.3788/fgxb20123309.1030
  中图分类号： O482.31
- 纸质出版日期：2012-9-10，
  
  收稿日期：2012-6-10，
  
  修回日期：2012-6-29，
扫描看全文
王海菊, 张文珠, 何成, 周硼. 基于FRET机理的荧光探针的合成及其对汞离子的识别[J]. 发光学报, 2012,33(9): 1030-1036

WANG Hai-ju, ZHANG Wen-zhu, HE Cheng, ZHOU Peng. Synthesis of Fluorescent Probe Based on FRET and Its Appalication in The Determination for Hg<sup>2+</sup>[J]. Chinese Journal of Luminescence, 2012,33(9): 1030-1036
王海菊, 张文珠, 何成, 周硼. 基于FRET机理的荧光探针的合成及其对汞离子的识别[J]. 发光学报, 2012,33(9): 1030-1036 DOI： 10.3788/fgxb20123309.1030.

WANG Hai-ju, ZHANG Wen-zhu, HE Cheng, ZHOU Peng. Synthesis of Fluorescent Probe Based on FRET and Its Appalication in The Determination for Hg<sup>2+</sup>[J]. Chinese Journal of Luminescence, 2012,33(9): 1030-1036 DOI： 10.3788/fgxb20123309.1030.

摘要

基于FRET机理设计合成了一个包含罗丹明6G及香豆素的汞离子荧光探针Rh-6G-coumarin(RC)

研究了它的光谱性能及对汞离子的识别作用。在

OH)∶

O)=9∶1溶液中加入汞离子后

575 nm处荧光强度迅速增大

荧光由蓝色变为明亮的红色

同时溶液的颜色由黄色变为红色。溶液中其他金属离子

如Na

、K

、Mg

、Fe

、Co

、Pb

、Zn

、Cd

和Cr

对汞离子的荧光识别没有太大影响。该探针可在较宽的pH ( 4~10)范围内识别汞离子。光谱滴定实验表明

汞离子与RC以2∶1的计量比形成了配合物。

Abstract

By combining rhodamine 6G and cumarin

a fluorescent probe of Hg

Rh-6G-cumarin(RC)

based on FRET mechanism was synthesized. And through research its spectral properties and recognition to mercury ion

we found that it featured a rapid fluorescence enhancement at 575 nm upon the addition of Hg

OH)∶

O)=9∶1 solution. The fluorescence changed from blue to bright red

while the color of the solution changed from yellow to red. This probe showed high specific for Hg

over other metal cations

such as Na

and Pb

. It could detect Hg

effectively in a relatively wide pH range (4~10). The titration experiment indicated a 2∶1 stoichiometry of the RC-Hg

complexation species.

关键词

荧光探针汞离子FRET罗丹明6G香豆素

Keywords

fluorescent probeHg2+FRETRhodamine 6Gcumarin

references

Cai S, Lao K M, Lau C, et al. "Turn-on" chemiluminescence sensor for the highly selective and ultrasensitive detection of Hg2+ ions based on interstrand cooperative coordination and catalytic formation of gold nanoparticles [J]. Anal. Chem., 2011, 83(24):9702-9708.

Nolan E M, Lippard S J. Tools and tactics for the optical detection of mercuric ion [J]. Chem. Rev., 2008, 108(9):3443-3480.

Lee Y H, Lee M H, Zhang J F, et al. Pyrene excimer-based calix

arene FRET chemosensor for mercury(Ⅱ) [J]. J. Org. Chem., 2010, 75(21):7159-7165.

Zhang Y N, Yang L L, Zhou P, et al. Fluorescent emission of coumaric aldehyde molecule enhanced by dihydrogen phosphate [J]. Chin. J. Lumin.(发光学报), 2011, 32(9):962-967 (in Chinese).

Ma Y S, Zhang F X, Zhang Y M, et al. Synthesis and characterization of a pH fluorescence probe based on water soluble perylene diinide derit [J]. Chin. J. Lumin.(发光学报), 2011, 32(3):251-255 (in Chinese).

Vázquez M, Fabbrizzi L, Taglietti A, et al. A colorimetric approach to anion sensing:A selective chemosensor of fluoride ions,in which color is generated by anion-enhanced delocalization [J]. Angew. Chem. Int. Ed., 2004, 43(15):1962-1965.

Ma Y S, Zhang F X, Sun Y M, et al. Synthesis and characterization of a pH fluorescence probe based water soluble perylene diimide derivative [J]. Chin. J. Lumin.(发光学报), 2011, 32(3):251-255 (in Chinese).

Dong J, Yu J, Feng S L, et al. A salen-type fluorescent probe to selectively identify Mg(Ⅱ) [J]. Chin. J. Lumin.(发光学报), 2012, 33(6):669-673 (in Chinese).

Liu B S, Xue C L, Wang J, et al. Studies on the interaction between ciprofloxacin and ofloxacin in presence of bovine serum albumin by spectroscopic methods [J]. Chin. J. Lumin.(发光学报), 2010, 31(2):285-290 (in Chinese).

Connors K A. Binding Contants [M]. New York: John Wiley, 1987.

Berberan-Santos M N, Choppinet P, Fedorov A, et al. Multiehromophoric cyelodextrins. 8. dynamies of homo- and heterotransfer of exeitation energy in inelusion complexes with fluoreseent dyes [J]. J. Am. Chem. Soc., 2000, 122(48):11876-11886.

Yang Y K, Yook K J, Tae J. A rhodamine-based fluoresecent and colorimetric chemodosimeter for the rapid detection of Hg2+ ions in aqueous media [J]. J. Am. Chem. Soc., 2005, 127(48):16760-16761.

Zhang X L, Xiao Y, Qian X H. Highly energy transfer in the light harvesting system composed of three kinds of boron-dipyrromethene derivatives [J]. Org. Lett., 2008, 10(1):29-32.

Zhang X L, Xiao Y, Qian X H. A ratiometric fluorescent probe based on FRET for imaging Hg2+ ions in living cells [J]. Angew. Chem. Int. Ed., 2008, 47(42):8025-8029.

Yuan L, Lin W Y, Chen B, et al. Development of FRET-based ratiometric fluorescent Cu2+ chemodosimeters and the applications for living cell imaging [J]. Org. Lett., 2012, 14(2):432-435.

Childress E S, Roberts C A, Sherwood D Y, et al. Ratiometric fluorescence detection of mercury ions in water by conjugated polymer nanoparticles [J]. Anal. Chem., 2012, 84(3):1235-1239.

浏览量

下载量

CSCD

文章被引用时，请邮件提醒。

提交

工具集

关联资源

一种新型近红外荧光探针用于Aβ40聚集体的检测

基于喹啉基的噁二唑酮类荧光探针合成及对Fe³⁺的识别

高荧光效率硫量子点的简单制备及其在对硝基苯酚检测中的应用

新型姜黄素衍生物荧光探针的合成及对半胱氨酸的选择性识别

一种线粒体靶向香豆素基pH荧光探针合成及性能