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大连理工大学化学学院 精细化工国家重点实验室,辽宁 大连,116021
纸质出版日期:2012-9-10,
收稿日期:2012-6-10,
修回日期:2012-6-29,
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王海菊, 张文珠, 何成, 周硼. 基于FRET机理的荧光探针的合成及其对汞离子的识别[J]. 发光学报, 2012,33(9): 1030-1036
WANG Hai-ju, ZHANG Wen-zhu, HE Cheng, ZHOU Peng. Synthesis of Fluorescent Probe Based on FRET and Its Appalication in The Determination for Hg<sup>2+</sup>[J]. Chinese Journal of Luminescence, 2012,33(9): 1030-1036
王海菊, 张文珠, 何成, 周硼. 基于FRET机理的荧光探针的合成及其对汞离子的识别[J]. 发光学报, 2012,33(9): 1030-1036 DOI: 10.3788/fgxb20123309.1030.
WANG Hai-ju, ZHANG Wen-zhu, HE Cheng, ZHOU Peng. Synthesis of Fluorescent Probe Based on FRET and Its Appalication in The Determination for Hg<sup>2+</sup>[J]. Chinese Journal of Luminescence, 2012,33(9): 1030-1036 DOI: 10.3788/fgxb20123309.1030.
基于FRET机理设计合成了一个包含罗丹明6G及香豆素的汞离子荧光探针Rh-6G-coumarin(RC)
研究了它的光谱性能及对汞离子的识别作用。在
V
(C
2
H
5
OH)∶
V
(H
2
O)=9∶1溶液中加入汞离子后
575 nm处荧光强度迅速增大
荧光由蓝色变为明亮的红色
同时溶液的颜色由黄色变为红色。溶液中其他金属离子
如Na
+
、K
+
、Mg
2+
、Fe
2+
、Co
2+
、Pb
2+
、Zn
2+
、Cd
2+
和Cr
3+
对汞离子的荧光识别没有太大影响。该探针可在较宽的pH ( 4~10)范围内识别汞离子。光谱滴定实验表明
汞离子与RC以2∶1的计量比形成了配合物。
By combining rhodamine 6G and cumarin
a fluorescent probe of Hg
2+
Rh-6G-cumarin(RC)
based on FRET mechanism was synthesized. And through research its spectral properties and recognition to mercury ion
we found that it featured a rapid fluorescence enhancement at 575 nm upon the addition of Hg
2+
in
V
(C
2
H
5
OH)∶
V
(H
2
O)=9∶1 solution. The fluorescence changed from blue to bright red
while the color of the solution changed from yellow to red. This probe showed high specific for Hg
2+
over other metal cations
such as Na
+
K
+
Mg
2+
Cr
3+
Fe
2+
Co
2+
Zn
2+
Cd
2+
and Pb
2+
. It could detect Hg
2+
effectively in a relatively wide pH range (4~10). The titration experiment indicated a 2∶1 stoichiometry of the RC-Hg
2+
complexation species.
荧光探针汞离子FRET罗丹明6G香豆素
fluorescent probeHg2+FRETRhodamine 6Gcumarin
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