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北京交通大学光电子技术研究所 教育部发光与光学信息重点实验室 北京,100044
纸质出版日期:2010-6-30,
网络出版日期:2010-6-30,
收稿日期:2010-1-10,
修回日期:1900-1-2,
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彭洪尚, 黄世华. 比率荧光纳米水凝胶的制备以及在pH探测中的应用[J]. 发光学报, 2010,31(3):395-399.
PENG Hong-shang, HUANG Shi-hua. A Ratiometric Fluorescense Nanogel for pH Sensor[J]. Chinese Journal of Luminescence, 2010,31(3):395-399.
基于再沉淀方法制备了一种具有比率荧光发射的纳米水凝胶
适用于检测生理范围的pH值。通过在聚氨酯水凝胶中引入pH值指示剂以及具有共振能量传递关系的荧光染料分子
赋予原本非pH响应和非荧光型的水凝胶以pH探测能力。随着pH值由酸性渐变为碱性
纳米水凝胶的绿色荧光强度逐渐增强
而红色荧光逐渐减弱。所制备的纳米水凝胶由于具有灵敏度高、亲水性好、稳定性好、响应快以及pH值检测范围恰好涵盖了生理pH值范围(pH值6~8)等优点
因此在细胞内pH值探测领域具有广阔应用前景。
In this paper a ratiometric fluorescence nanogel was prepared and used in pH sensing. By introducing a pH probe and two fluorescent dyes (related by fluorescent resonant energy transfer)
the non-responsive and non-fluorescent hydrogel (polyurathane) was imparted the ability of pH sensing. With the increase of pH value from acidic to basic
the dominant fluorescence of nanogels varieds from red to green accordingly. The as-prepared nanogels exhibits high sensitivity
high hydrophilicity
quick responsiveness
and physiological pH detecting in the range of 6~8. Hence
the nanogels would be very potential as intracellular pH nanosensors.
纳米水凝胶pH探针比率荧光共振能量传递
nanogelpH sensorratiometric fluorescenceFRET
. Gehrke S H. Synthesis, equilibrium swelling, kinetics, permeability and applications of environmentally responsive gels [J]. Adv. Polym. Sci., 1993, 110 :81-144.
. Hoffman A S. Hydrogels for biomedical applications [J]. Adv. Drug Delivery Rev., 2002, 54 (1):3-12.
. Holtz J H, Asher S A. Polymerized colloidal crystal hydrogel films as intelligent chemical sensing materials [J]. Nature, 1997, 389 (6653):829-832.
. Kim J, Nayak S, Lyon L A. Bioresponsive hydrogel microlenses [J]. J. Am. Chem. Soc., 2005, 127 (26):9588-9592.
. Sirkar K, Revzin A,Pishko M V. Glucose and lactate biosensors based on redox polymer/oxidoreductase nanocomposite thin films [J]. Anal. Chem., 2000, 72 (13):2930-2936.
. Iwai K, Matsumura Y, Uchiyama S, et al. Development of fluorescent microgel thermometers based on thermo-responsive polymers and their modulation of sensitive range [J]. J. Mat. Chem., 2005, 15 (27-28):2796-2800.
. Gota C, Uchiyama S, Yoshihara T, et al. Temperature-dependent fluorescence lifetime of a fluorescent polymeric thermometer, poly(N-isopropylacrylamide), labeled by polarity and hydrogen bonding sensitive 4-sulfamoyl-7-aminobenzofurazan [J]. J. Phys. Chem. B, 2008, 112 (10):2829-2836.
. Gota C, Okabe K, Funatsu T, et al. Hydrophilic fluorescent nanogel thermometer for intracellular thermometry [J]. J. Am. Chem. Soc., 2009, 131 (8):2766-2767.
. Yoo M K, Jang M K, Nah J W, et al. Effect of temperature on the mobility of core-shell-type nanoparticles composed of poly(g-benzyl-L-glutamate) and poly(N-isopropylacrylamide) [J]. Macromol. Chem. Phys., 2006, 207 (5):528-535.
. Missirlis D, Tirelli N, Hubbell J A. Amphiphilic hydrogel nanoparticles. Preparation, characterization and preliminary assessment as new colloidal drug carriers [J]. Langmuir, 2005, 21 (6):2605-2613.
. Oh J K, Siegwart D J, Lee H I, et al. Biodegradable nanogels prepared by atom transfer radical polymerization as potential drug delivery carriers: synthesis, biodegradation, in vitro release, and bioconjugation [J]. J. Am. Chem. Soc., 2007, 129 (18):5939-5945.
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