水溶性β-NaY(Gd)F₄:Yb³⁺/Er³⁺纳米棒的水热合成及生物偶联

水溶性-NaY(Gd)F 4 :Yb 3+ /Er 3+ 纳米棒的水热合成及生物偶联 Hydrothermal Synthesis and Bioconjugation of Water-soluble -NaY(Gd)F 4 :Yb 3+ /Er 3+ Nanorods 肇欣 ZHAO Xin 1 2 first-author 孙振刚 SUN Zhen-gang 1 张伟 ZHANG Wei 2 华瑞年 HUA Rui-nian 2 辽宁师范大学 化学化工学院,辽宁 大连,116029 辽宁师范大学 化学化工学院,辽宁 大连,116029 School of Chemistry and Chemical Engineering, Liaoning Normal University School of Chemistry and Chemical Engineering, Liaoning Normal University 大连民族学院 生命科学学院,辽宁 大连,116600 大连民族学院 生命科学学院,辽宁 大连,116600 College of Life Science, Dalian Nationalities University College of Life Science, Dalian Nationalities University 以聚乙烯亚胺(PEI)为表面活性剂,采用水热合成法,制得了表面氨基修饰的水溶性-NaY(Gd)F 4 :Yb 3+ /Er 3+ 纳米棒,并对-NaY(Gd)F 4 :Yb 3+ /Er 3+ 上转换纳米棒的制备方法、条件等进行了考察.结果表明,当Gd 3+ 的引入摩尔分数为40%时,200 ℃下反应8 h即可获得纯-NaY(Gd)F 4 :Yb 3+ /Er 3+ 纳米棒.利用X射线粉末衍射仪(XRD)、扫描电子显微镜 (SEM)、透射电子显微镜(TEM)、荧光光谱(PL)对样品的结构、形貌及光谱特性进行了表征.结构和形貌分析结果表明,制得的样品为单相-NaY(Gd)F 4 :Yb 3+ /Er 3+ 纳米棒,纳米棒的截面粒径约为40 nm,平均长度约为210 nm.在980 nm波长激发下,样品的上转换发光光谱中出现了4个发射中心位于407,529,546,660 nm的发射带,分别对应于Er 3+ 离子的 2 H 9/2 4 I 15/2 、 2 H 11/2 4 I 15/2 、 4 S 3/2 4 I 15/2 和 4 F 9/2 4 I 15/2 跃迁.采用戊二醛法,使-NaY(Gd)F 4 :Yb 3+ /Er 3+ 上转换纳米棒表面的氨基与牛血清蛋白(BSA)分子中的氨基成功偶连在一起.利用紫外光谱分析(UV)和考马斯亮蓝法,对二者的偶联进行了证明. The amino modified -NaY(Gd)F 4 :Yb 3+ /Er 3+ nanorods were synthesized by hydrothermal synthesis process using polyethyleneimine (PEI) as a surfactant. The preparation method and conditions of -NaY(Gd)F 4 :Yb 3+ /Er 3+ upconversion nanorods were also studied. The pure -NaY(Gd)F 4 :Yb 3+ /Er 3+ upconversion nanorods can be obtained at 200 ℃ for 8 h with Gd 3+ mole fraction of 40%. The crystal structure, morphology and luminescent properties of final products were characterized by X-ray diffraction (XRD), field emission scanning electron microscopy (SEM), transmission electron microscopy (TEM) and fluorescence spectra (PL), respectively. The as-synthesized samples presented a pure phase -NaY(Gd)F 4 :Yb 3+ /Er 3+ nanorods with average diameter of 40 nm and average length of 210 nm. Under 980 nm excitation, four upconversion emissions centered at 407, 529, 546, 660 nm were observed, corresponding to 2 H 9 /2 4 I 15/2 , 2 H 11/2 4 I 15/2 , 4 S 3/2 4 I 15/2 and 4 F 9/2 4 I 15/2 transitions of Er 3+ , respectively. The amino groups on the surface of -NaY(Gd)F 4 :Yb 3+ /Er 3+ upconversion nanorods can be coupled with bovine serum albumin (BSA) via a glutaraldehyde method, and the coupling was confirmed by UV spectroscopy and Coomassie brilliant blue method at room temperature. &beta -NaY(Gd)F 4 :Yb 3+ /Er 3+ 上转换发光 生物偶联 &beta -NaY(Gd)F 4 :Yb 3+ /Er 3+ up-conversion luminescence bioconjugation Li C X, Lin J. Rare earth fluoride nano-/microcrystals: Synthesis, surface modification and application . J. Mater. Chem., 2010, 20: 6831-6847. Shen J, Sun L D, Yan C H. Luminescent rare earth nanomaterials for bioprobe applications [J]. Dalton Trans., 2008(42): 5687-5697. Qin W P, Zhang D S, Zhao D, et al. 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