碳量子点/曙红Y比率型荧光探针测定L-抗坏血酸

武文波; 张越诚; 李承佳; 马红燕; 杨晓军

doi:10.3788/fgxb20204103.0331

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碳量子点/曙红Y比率型荧光探针测定L-抗坏血酸

发光学应用及交叉前沿 | 更新时间：2020-08-12

- 碳量子点/曙红Y比率型荧光探针测定L-抗坏血酸
- Determination of L-ascorbic Acid by Carbon Quantum Dot/Eosin Y Ratiometric Fluorescence Probe
- 发光学报 2020年41卷第3期页码：331-338
- 作者机构：
  
  延安大学化学与化工学院延安市分析技术与检测重点实验室,陕西延安,716000
- 作者简介：
- 基金信息：
  
  延安大学科研计划青年基金（YDQ2018-17）();国家自然科学基金（31860089）资助项目()
- DOI：10.3788/fgxb20204103.0331
  中图分类号： O657.3
- 纸质出版日期：2020-3-5，
  
  网络出版日期：2019-10-15，
  
  收稿日期：2019-9-16，
  
  修回日期：2019-10-9，
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武文波, 张越诚, 李承佳等. 碳量子点/曙红Y比率型荧光探针测定L-抗坏血酸[J]. 发光学报, 2020,41(3): 331-338

WU Wen-bo, ZHANG Yue-cheng, LI Cheng-jia etc. Determination of L-ascorbic Acid by Carbon Quantum Dot/Eosin Y Ratiometric Fluorescence Probe[J]. Chinese Journal of Luminescence, 2020,41(3): 331-338
武文波, 张越诚, 李承佳等. 碳量子点/曙红Y比率型荧光探针测定L-抗坏血酸[J]. 发光学报, 2020,41(3): 331-338 DOI： 10.3788/fgxb20204103.0331.

WU Wen-bo, ZHANG Yue-cheng, LI Cheng-jia etc. Determination of L-ascorbic Acid by Carbon Quantum Dot/Eosin Y Ratiometric Fluorescence Probe[J]. Chinese Journal of Luminescence, 2020,41(3): 331-338 DOI： 10.3788/fgxb20204103.0331.

摘要

以天然生物质去皮的蓖麻为碳源，采用一步水热法合成了荧光性能优良的绿色荧光蓖麻碳量子点（CO-CQDs），对其形貌和发光性能进行了表征。通过将该CO-CQDs与荧光极强的卤代荧光素染料曙红Y（EY）复合，二者可形成荧光发射峰相距较远的新型CO-CQDs/EY复合物。在pH=4.00的Na

HPO

-柠檬酸缓冲溶液中，在320 nm的激发波长下，CO-CQDs/EY复合物于405 nm和540 nm处显示出两个独立的荧光发射峰。在该体系中加入Cr（Ⅵ），405 nm和540 nm两处的荧光信号均显著猝灭。L-抗坏血酸（L-ascorbic acid，AA）的加入可使复合物于540 nm的荧光信号恢复，而405 nm处的荧光强度基本不变。据此建立了一种以CO-CQDs/EY复合物为比率型荧光探针测定AA的新方法。实验测定了荧光信号恢复的最佳条件和影响荧光恢复的因素，初步探讨了反应机理。在优化的实验条件下，该探针于540 nm/405 nm两处的荧光强度比值与AA的浓度在5.010

-8

~4.010

-6

mol/L范围内呈良好线性关系，检出限为3.710

-8

mol/L。该探针用于检测药物、水果和蔬菜中AA的含量，结果满意。

Abstract

Green fluorescent castor carbon quantum dots(CO-CQDs) with excellent fluorescence properties were synthesized by one-step hydrothermal method with natural biomass peeled castor. By combining the as-synthesized CO-CQDs with eosin Y(EY)

a novel ratiometric fluorescence probe CO-CQDs/EY was formed. In the Na

HPO

-citric acid buffer solution of pH=4.00

under the excitation wavelength of 320 nm

the CO-CQDs/EY complex showed two independent fluorescence emission peaks at 405 nm and 540 nm. The fluorescence signals of 405 nm and 540 nm could be both quenched by Cr(Ⅵ). In the presence of L-ascorbic acid

the fluorescence signal at 540 nm was significantly increased

while the fluorescence intensity at 405 nm still remained unchanged. Therefore

a novel method for L-ascorbic acid analysis was developed based on this fluorescence ratiometric probe CO-CQDs/EY. This ratiometric method showed excellent specificity

sensitivity and accuracy. The optimum conditions of experiment were investigated and the mechanism of interaction was discussed. Under the optimized experimental conditions

the linear range of this method was 5.010

-8

-4.010

-6

mol/L

and the detection limit was calculated to be 3.710

-8

mol/L. Moreover

this method has been successfully applied to L-ascorbic acid analysis in drugs

vegetables and fruits

showing great potential in real sample analysis.

关键词

碳量子点曙红YCr(Ⅵ)比率型荧光探针L-抗坏血酸

Keywords

carbon quantum dotseosin YCr(Ⅵ)ratiometric fluorescence probeL-ascorbic acid

references

SMIRNOFF N. Ascorbic acid metabolism and functions:a comparison of plants and mammals[J]. Free Radic. Biol. Med., 2018,122:116-129.

孙振艳,赵中一,郭小慧,等. 荧光分析法测定维生素C[J]. 化学分析计量, 2006,15(4):18-20. SUN Z Y,ZHAO Z Y,GUO X H,et al.. Determination of vitamin C by fluorescence spectrophotometry[J]. Chem. Anal. Meter., 2006,15(4):18-20. (in Chinese).

张进,万瑞英,姚思童,等. FTIR定量分析维生素C制剂的制样方法研究[J]. 分析试验室, 2015,34(11):1353-1357. ZHANG J,WAN R Y,YAO S T,et al.. Study of sample preparation for determination of vitamin C preparations by Fourier transform infrared spectroscopy[J]. Chin. J. Anal. Lab., 2015,34(11):1353-1357. (in Chinese)

甘振威,武广恒,刘国良,等. 自动电位滴定法和高效液相色谱法测定深颜色果蔬中维生素C浓度的比较[J]. 吉林大学学报(医学版), 2014,40(2):441-445. GAN Z W,WU G H,LIU G L,et al.. Comparison of vitamin C concentrations in dark colour fruits and vegetables detected by automatic potentiometric titration and HPLC method[J]. J. Jilin Univ. (Med. Ed.), 2014,40(2):441-445. (in Chinese)

王蕊,姜丽,袁航,等. 高效液相色谱法测定维生素C片的含量[J]. 黑龙江医药, 2010,23(6):876-877. WANG R,JIANG L,YUAN H,et al.. Determination of vitamin C tablets by high performance liquid chromatography[J]. Heilongjiang Med. J., 2010,23(6):876-877. (in Chinese)

SAMIDE A,TUTUNARU B. Interactions between vitamin C and nanocolloidal silver particles studied by cyclic voltammetry and UV-Vis spectrophotometry[J]. Electroanalysis, 2017,29(11):2498-2506.

FARAJZADEH M A,NAGIZADEH S. A new simple and accurate turbidimetric method for determination of ascorbic acid in pharmaceuticals and fruits[J]. J. Chin. Chem. Soc., 2002,49(5):949-956.

BEHFAR A A,SADEGHI N,JANNAT B,et al.. Determination of L-ascorbic acid in plasma by voltammetric method[J]. Iran. J. Pharm. Res., 2010,9(2):123-128.

KLEPO L,COPRA-JANICIJEVIC A,KUKOC-MODUN L. A new indirect spectrofluorimetric method for determination of ascorbic acid with 2,4,6-tripyridyl-S-triazine in pharmaceutical samples[J]. Molecules, 2016,21(1):101-103.

WU F S,SU H F,WANG K,et al.. Facile synthesis of N-rich carbon quantum dots from porphyrins as efficient probes for bioimaging and biosensing in living cells[J]. Int. J. Nanomed., 2017,12:7375-7391.

张越诚,马红燕,王靖原,等. 基于糊精碳量子点荧光信号"关-开"测定L-抗坏血酸的研究[J]. 化学研究与应用, 2019,31(4):638-643. ZHANG Y C,MA H Y,WANG J Y,et al.. Sensitive detection of L-ascorbic acid based on "off-on" dextrin carbon quantum dots as fluorescent probe[J]. Chem. Res. Appl., 2019,31(4):638-643. (in Chinese)

LIU J J,CHEN Y L,WANG W F,et al.. "Switch-on" fluorescent sensing of ascorbic acid in food samples based on carbon quantum dots-MnO2 probe[J]. J. Agric. Food Chem., 2016,64(1):371-380.

YAN F Y,BAI Z J,LIU F,et al.. Ratiometric fluorescence probes based on carbon dots[J]. Curr. Org. Chem., 2018,22(1):57-66.

ZHOU H T,MEI J,CHEN Y A,et al.. Phenazine-based ratiometric Hg2+ probes with well-resolved dual emissions:a new sensing mechanism by vibration-induced emission(VIE)[J]. Small, 2016,12(47):6542-6546.

YANG S H,GUO W J,SUN X H. Electrostatic association complex of a polymer capped CdTe(S) quantum dot and a small molecule dye as a robust ratiometric fluorescence probe of copper ions[J]. Dyes Pigm., 2018,158:114-120.

WANG C X,HUANG Y J,JIANG K L,et al.. Dual-emitting quantum dot/carbon nanodot-based nanoprobe for selective and sensitive detection of Fe3+ in cells[J]. Analyst, 2016,141(14):4488-4494.

刘雯. 比率荧光传感体系的构建及其在快速检测方面的应用[D]. 济南:山东师范大学, 2018. LIU W. Construction of Ratio Fluorescence Sensing System and Its Application in Rapid Detection[D]. Jinan:Shandong Normal University, 2018. (in Chinese)

LI G H,LIU Y,NIU J,et al.. A ratiometric fluorescent composite nanomaterial for RNA detection based on graphene quantum dots and molecular probes[J]. J. Mater. Chem. B, 2018,6(26):4380-4384.

YANG Y H,CUI J H,ZHENG M T,et al.. One-step synthesis of amino-functionalized fluorescent carbon nanoparticles by hydrothermal carbonization of chitosan[J]. Chem. Commun., 2012,48(3):380-382.

朱欣欣. 碳量子点/有机染料复合物比率荧光探针的制备与分析应用研究[D]. 青岛:青岛大学, 2017. ZHU X X. Preparation and Analysis of Carbon Quantum Dot/Organic Dye Complex Ratio Fluorescent Probe[D]. Qingdao:Qingdao University, 2017. (in Chinese)

YU L H,LIU Z F,LIU S P. Fading spectrophotometric method for the determination of polyvinylpyrrolidone with eosin Y[J]. Chin. J. Chem., 2009,27(8):1505-1509.

田伦富,胡小莉,刘忠芳,等. 普罗帕酮与曙红Y的双荧光猝灭反应及其分析应用研究[J]. 化学学报, 2011,69(4):411-417. TIAN L F,HU X L,LIU Z F,et al.. Mutual fluorescence quenching between propafenone and eosin Y and its analytical application[J]. Acta Chim. Sinica, 2011,69(4):411-417. (in Chinese)

龚未彬,杨传孝,孙向英. 荧光聚乙烯亚胺/曙红X比率荧光法测定十二烷基硫酸钠[J]. 华侨大学学报(自然科学版), 2016,37(3):336-341. GONG W B,YANG C X,SUN X Y. Detection of sodium dodecyl sulfate by ratiometric fluorescence based on fluorescence polyethyleneimine/eosin Y[J]. J. Huaqiao Univ.(Nat. Sci.), 2016,37(3):336-341. (in Chinese)

白鹏霞. 碳量子点荧光探针的制备及在Cr(Ⅵ)与甲醛检测中的应用[D]. 西安:陕西科技大学, 2019. BAI P X. Preparation of Carbon Ouantum Dot Fluorescent Probe and Its Application in Detectionof Cr(Ⅵ) and HCHO[D]. Xi'an:Shaanxi University of Science & Technology, 2019. (in Chinese)

ZHANG X Q,MA Z Y,YANG Y,et al.. Influence of alkyl length on properties of piezofluorochromic aggregation induced emission compounds derived from 9,10-bis[(N-alkylphenothiazin-3-yl) vinyl] anthracene[J]. Tetrahedron, 2014,70(4):924-929.

刘真真,齐沛沛,王新全,等. 超高效液相色谱-四极杆-飞行时间质谱测定果蔬中维生素C[J]. 色谱, 2016,34(11):1048-1054. LIU Z Z,QI P P,WANG X Q,et al.. Determination of vitamin C in fruits and vegetables by ultra performance liquid chromatography-quadrupole time-of-flight mass spectrometry[J]. Chin. J. Chromatogr., 2016,34(11):1048-1054. (in Chinese)

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