基于萘酰腙类锌离子增强型荧光探针的合成及光谱性能

吴红梅; 郭宇; 曹建芳; 吴中立

doi:10.3788/fgxb20183905.0621

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基于萘酰腙类锌离子增强型荧光探针的合成及光谱性能

材料合成及性能 | 更新时间：2020-08-12

- 基于萘酰腙类锌离子增强型荧光探针的合成及光谱性能
- Synthesis and Spectral Properties of Zinc Ion-enhanced Fluorescence Probe Based on Naphthoyl Acylhydrazone
- 发光学报 2018年39卷第5期页码：621-626
- 作者机构：
  
  1. 辽宁工业大学化学与环境工程学院,辽宁锦州,121001
  2. 辽宁工业大学机械工程与自动化学院, 辽宁锦州 121001
- 作者简介：
- 基金信息：
  
  国家自然科学基金（21601075，21606118）();辽宁省自然科学基金（2015020249）资助项目()
- DOI：10.3788/fgxb20183905.0621
  中图分类号： O433.5
- 纸质出版日期：2018-5-5，
  
  网络出版日期：2017-10-25，
  
  收稿日期：2017-9-14，
  
  修回日期：2018-1-11，
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吴红梅, 郭宇, 曹建芳等. 基于萘酰腙类锌离子增强型荧光探针的合成及光谱性能[J]. 发光学报, 2018,39(5): 621-626

WU Hong-mei, GUO Yu, CAO Jian-fang etc. Synthesis and Spectral Properties of Zinc Ion-enhanced Fluorescence Probe Based on Naphthoyl Acylhydrazone[J]. Chinese Journal of Luminescence, 2018,39(5): 621-626
吴红梅, 郭宇, 曹建芳等. 基于萘酰腙类锌离子增强型荧光探针的合成及光谱性能[J]. 发光学报, 2018,39(5): 621-626 DOI： 10.3788/fgxb20183905.0621.

WU Hong-mei, GUO Yu, CAO Jian-fang etc. Synthesis and Spectral Properties of Zinc Ion-enhanced Fluorescence Probe Based on Naphthoyl Acylhydrazone[J]. Chinese Journal of Luminescence, 2018,39(5): 621-626 DOI： 10.3788/fgxb20183905.0621.

摘要

设计合成了一种简单的酰腙类结构的增强型荧光探针HM，实现了对锌离子（Zn

）的高选择性识别。运用ESI-MS质谱、荧光光谱和紫外-可见光谱等手段研究了探针HM对Zn

的识别过程。紫外光谱测试表明，当向探针HM中加入Zn

后，386 nm处的吸收峰逐渐减弱，在420 nm处出现了新的吸收峰，并且强度逐渐增大，直至达到平衡，等吸收点为396 nm。荧光光谱分析表明，探针HM能够高选择性地识别Zn

。在发射波长510 nm处的荧光增强2.5倍，最低检出限为1.010

-5

mol/L、量子产率为0.02。该识别过程为PET（光诱导电子转移机理）和CHEF（螯合荧光增强机理）共同作用的结果。通过电喷雾质谱和Job's plot实验证明探针HM与Zn

以1：1配位，平衡常数（

）达到4.0510

Lmol

-1

。

Abstract

A simple fluorescent probe HM based on acylhydrazone structure was synthesized for recognition of zinc ion (Zn

). The recognition process of Zn

was studied by ESI-MS

fluorescence spectroscopy and UV-Vis spectroscopy. UV-Vis titration test shows that the addition of Zn

into HM aqueous solution caused gradually drop of the absorption peak at 386 nm. At the same time

a new absorption peak at 420 nm appeared and the intensity gradually increased until equilibrium. A sharp isosbestic point at 396 nm was obtained. Florescence spectroscopy indicates that the fluorescent probe HM exhibites high selectivity for detection of Zn

. The fluorescence intensity was increased by 2.5 times at excited wavelength of 510 nm. The lowest detection limit of HM for Zn

is up to 1.010

-5

mol/L and the fluorescence quantum yield is 0.02. The recognition process is presumed to be caused by PET (photoinduced electron transfer) process combining with CHEF (Chelation enhanced fluorescence) mechanism. The Job's plot and ESI-MS results demonstrate a 1:1 stoichiometric host-guest complex for probe molecular HM and Zn

with the association constant being calculated as

=4.0510

Lmol

-1

关键词

锌离子荧光探针酰腙识别

Keywords

zinc ionfluorescence probeacylhydrazonerecognition

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