基于亚甲基蓝的近红外荧光探针用于HOCl的特异性检测

姚书帆; 尧雨斯; 郑武斌; 叶晨喆; 应杰; 吕光磊; 李春霞

doi:10.37188/fgxb20204107.0791

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基于亚甲基蓝的近红外荧光探针用于HOCl的特异性检测

材料合成及性能 | 更新时间：2020-09-10

- 基于亚甲基蓝的近红外荧光探针用于HOCl的特异性检测
- Near-infrared Fluorescent Probe Based on Methylene Blue for Specific Detection of Hypochlorous Acid
- 发光学报 2020年41卷第7期页码：791-799
- 作者机构：
  
  浙江师范大学化学与生命科学学院, 浙江金华 321004
- 作者简介：
  
  [ "姚书帆 (1998-)，女，浙江绍兴人，在读本科生，主要从事有机荧光探针分子的合成及其在疾病检测中应用的研究。E-mail: yaoshufan@zjnu.edu.cn" ]
  [ "吕光磊(1986-)，男，山东泰安人，博士，讲师，2017年于复旦大学获得博士学位，主要从事有机无机杂化材料的设计合成及其在疾病诊疗中应用的研究。E-mail:guanglei@zjnu.edu.cn" ]
  [ "李春霞 (1977-)，女，山东德州人，博士，教授，硕士研究生导师/博士研究生导师，2008年于中国科学院长春应用化学研究所获得博士学位，主要从事功能纳米材料在荧光检测和肿瘤诊疗中的研究。E-mail: cxli@zjnu.edu.cn" ]
- 基金信息：
  
  国家自然科学基金面上项目(51872263);浙江省自然科学基金重点项目(LZ19E020001);浙江省自然科学基金重点项目青年项目(LQ19B050003)
- DOI：10.37188/fgxb20204107.0791
  中图分类号： O656
- 纸质出版日期：2020-7，
  
  收稿日期：2020-5-5，
  
  录用日期：2020-5-25
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姚书帆, 尧雨斯, 郑武斌, 等. 基于亚甲基蓝的近红外荧光探针用于HOCl的特异性检测[J]. 发光学报, 2020,41(7):791-799.

Shu-fan YAO, Yu-si YAO, Wu-bin ZHENG, et al. Near-infrared Fluorescent Probe Based on Methylene Blue for Specific Detection of Hypochlorous Acid[J]. Chinese Journal of Luminescence, 2020,41(7):791-799.
姚书帆, 尧雨斯, 郑武斌, 等. 基于亚甲基蓝的近红外荧光探针用于HOCl的特异性检测[J]. 发光学报, 2020,41(7):791-799. DOI： 10.37188/fgxb20204107.0791.

Shu-fan YAO, Yu-si YAO, Wu-bin ZHENG, et al. Near-infrared Fluorescent Probe Based on Methylene Blue for Specific Detection of Hypochlorous Acid[J]. Chinese Journal of Luminescence, 2020,41(7):791-799. DOI： 10.37188/fgxb20204107.0791.

摘要

次氯酸（HOCl）是一种由过氧化氢和氯离子在髓过氧化物酶（MPO）催化作用下产生的活性氧。由于其在机体抵抗病原体的免疫防御中起着至关重要的作用，因此对HOCl的识别和检测具有非常重要的意义。目前，检测HOCl的方法有电分析法、色谱法、化学发光法和荧光分析法，其中荧光检测法以其简单、快速、高选择性、高灵敏度和实时检测等突出优点引起了许多研究者的兴趣。本文以亚甲基蓝（Methylene blue，MB）为荧光母核，设计合成了一种近红外荧光探针

MB-1

用于HOCl的特异性检测。该探针可在体外特异性检测HOCl，响应之后，荧光会有显著的增强，同时伴有溶液颜色从无色到蓝色的明显变化。该探针对HOCl具有较高的灵敏度，其检测限为8.2 nmol/L。此外，该探针具有较好的抗干扰能力，为在生理水平上检测HOCl提供了可能。

Abstract

Hypochlorous acid (HOCl) is a reactive oxygen species produced by hydrogen peroxide and chloride ions under the catalysis of myeloperoxidase (MPO). HOCl plays an important role in immune defense against pathogens. Therefore

it is of great significance to sense and detect HOCl. There are several methods for the detection of HOCl

such as electroanalysis

chromatography

chemiluminescence and fluorescence analysis and so on. Currently

fluorescence imaging technique has attracted many researchers' interest because of its advantages

including high selectivity and sensitivity

easy operation

quick response

and real-time detection. In this paper

a methylene blue-based near-infrared fluorescence probe

MB-1

was designed and synthesized for the fast and specific detection of HOCl. The probe can specifically detect HOCl

in vitro

accompanied by a significant enhancement of near-infrared fluorescence intensity and a remarkable colour change from colorless to blue. Moreover

the probe displayed high sensitivity towards HOCl and limit of detection (LOD) was as low as 8.2 nmol/L. In addition

the probe showed good anti-interference ability

indicating that it has the potential to detect HOCl at a physiological level.

关键词

次氯酸荧光探针活性氧亚甲基蓝近红外

Keywords

hypochlorous acidfluorescent probesreactive oxygen speciesmethylene bluenear-infrared

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