黑色素瘤循环肿瘤细胞检测的金属卤化物钙钛矿外泌体复合探针检测新策略

陈志山; 徐朋飞; 张绍安; 李杨

doi:10.37188/CJL.20230237

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黑色素瘤循环肿瘤细胞检测的金属卤化物钙钛矿外泌体复合探针检测新策略

更新时间：2023-11-16

- 黑色素瘤循环肿瘤细胞检测的金属卤化物钙钛矿外泌体复合探针检测新策略
  增强出版
- Exosome-Triggered Metal Halide Perovskite- Mediated Strategy of Circulating Tumors Cells Detection for Melanoma
- 发光学报 2023年页码：1-12
- 作者机构：
  
  1.广州医科大学生物医学工程学院，广东广州　511436
- 作者简介：
  
  [ "陈志山（1993-），男，广东汕尾人，博士研究生，2020年于广州医科大学获得硕士学位，主要从事荧光纳米材料体外检测方面的研究。Email： zhishan_chen@qq.com" ]
  [ "徐朋飞（1996-），男，江苏宿迁人，硕士研究生，2023年于广州医科大学获得硕士学位，主要从事荧光纳米材料体外检测方面的研究。Email： 2432368710@qq.com" ]
  [ "李杨（1984-），男，山东淄博人，博士，教授，博士研究生导师，2014年于华南理工大学获得博士学位，主要从事无机固体发光材料缺陷调控，特别是长余辉材料的设计、机理及应用的研究。Email： lychris@sina.com" ]
- 基金信息：
  
  Foundation:This work was financially supported by the National Natural Science Foundation of China （国家自然科学基金）(Grant 52172083);International Science & Technology Cooperation Program of Guangdong （广东省国际科技合作计划）(Grant 2021A0505030078);Guangzhou Key Research and Development Program （广州市重点研发计划）(Grant 2023B03J1239);Program for Innovative Research Team in University of Education System of Guangzhou （广州市教育系统创新学术团队计划）(Grant 202235404);China Postdoctoral Science Foundation （中国博士后科学基金）(Grant 2022M720919)
- DOI：10.37188/CJL.20230237
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陈志山,徐朋飞,张绍安等.黑色素瘤循环肿瘤细胞检测的金属卤化物钙钛矿外泌体复合探针检测新策略[J].发光学报,

CHEN Zhishan,XU Pengfei,ZHANG Shaoan,et al.Exosome-Triggered Metal Halide Perovskite- Mediated Strategy of Circulating Tumors Cells Detection for Melanoma[J].Chinese Journal of Luminescence,
陈志山,徐朋飞,张绍安等.黑色素瘤循环肿瘤细胞检测的金属卤化物钙钛矿外泌体复合探针检测新策略[J].发光学报, DOI：10.37188/CJL.20230237

CHEN Zhishan,XU Pengfei,ZHANG Shaoan,et al.Exosome-Triggered Metal Halide Perovskite- Mediated Strategy of Circulating Tumors Cells Detection for Melanoma[J].Chinese Journal of Luminescence, DOI：10.37188/CJL.20230237

摘要

液体活检技术的兴起为黑色素瘤的快速、准确诊断开辟了新的机遇。然而，普通循环肿瘤细胞活检基于上皮黏附蛋白进行阳性富集，但信号标记的有机荧光探针存在量子效率低，导致检测黑色素瘤循环肿瘤细胞时准确率和灵敏度较低。本文以高量子效率的金属卤化物钙钛矿量子点作为信号标记物，以黑色素瘤来源的外泌体作为生物识别分子，构建了一种用于黑色素瘤液体活检的循环肿瘤细胞检测新策略。与商品化的上皮细胞黏附蛋白富集策略相比，本研究报道的复合探针检测新策略，其检测灵敏度提高了一个数量级，并且具有良好的亲水性和低毒性。实验结果证明了外泌体引导的金属卤化物钙钛矿量子点指示的黑色素瘤循环肿瘤细胞检测新策略具有理想的应用前景。

Abstract

The emerging technique of liquid biopsy opens up a new opportunity for the rapid and accurate diagnosis of melanoma tumor featuring high metastatic tendency and mortality rates. However， the accuracy and sensitivity for liquid biopsy of melanoma are still limited， due to the use of low-expression epithelial cell adhesion molecule（EpCAM） to achieve enrichment of circulating tumor cells（CTCs） and subaltern photoluminescence quantum yields（PLQYs） organic probes as signal indicators. Here， we construct a new strategy of CTCs detection for liquid biopsy of melanoma by employing high-PLQYs metal halide perovskite（MHP） quantum dots and melanoma-derived exosome（MEX） as signal indicators and recognition analytes， respectively. The synthesized composites（PSPE） exhibit the superiorities on perfect hydrophilicity and hypotoxicity. In contrast to commercial EpCAM-triggered products， the detection sensitivity of our reagent materials is raised up by an order of magnitude. All results predict the potential of exosome-triggered MHP-mediated CTCs detection in accurate melanoma diagnosis of liquid biopsy.

关键词

黑色素瘤外泌体循环肿瘤细胞金属卤化物钙钛矿液体活检

Keywords

melanomaexosomecirculating tumor cellsmetal halide perovskiteliquid biopsy

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