Hg<sup>2+</sup>调控氮磷共掺杂碳纳米点在半胱氨酸/同型半胱氨酸荧光检测中的应用

路露; 徐律; 张鑫; 周小红; 余乐平

doi:10.37188/CJL.20210389

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Hg²⁺调控氮磷共掺杂碳纳米点在半胱氨酸/同型半胱氨酸荧光检测中的应用

发光学应用及交叉前沿 | 更新时间：2022-04-07

- Hg²⁺调控氮磷共掺杂碳纳米点在半胱氨酸/同型半胱氨酸荧光检测中的应用
  增强出版
- Fluorescent Detection for Cysteine/Homocysteine Using Hg²⁺ Modulated Nitrogen and Phosphorus Co-doped Carbon Nanodots
- 发光学报 2022年43卷第3期页码：440-452
- 作者机构：
  
  1.无锡商业职业技术学院　汽车技术学院，江苏　无锡　214153
  2.盐城工学院　材料科学与工程学院，江苏　盐城　224002
- 作者简介：
  
  [ "路露(1988-)，女，江苏无锡人，博士，讲师，2018年于东南大学获得博士学位，主要从事纳米材料的光电研究与应用。E-mail: lulu2143@126.com" ]
- 基金信息：
  
  江苏省自然科学基金(BK20191041);江苏省高校自然科学基金面上项目(19KJB480011);无锡商业职业技术学院培育类课题(KJXJ21604);无锡商业职业技术学院产教融合项目(XQ202113)
- DOI：10.37188/CJL.20210389
  中图分类号：
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路露, 徐律, 张鑫, 等. Hg²⁺调控氮磷共掺杂碳纳米点在半胱氨酸/同型半胱氨酸荧光检测中的应用[J]. 发光学报, 2022,43(3):440-452.

Lu LU, Lü XU, Xin ZHANG, et al. Fluorescent Detection for Cysteine/Homocysteine Using Hg²⁺ Modulated Nitrogen and Phosphorus Co-doped Carbon Nanodots[J]. Chinese Journal of Luminescence, 2022,43(3):440-452.
路露, 徐律, 张鑫, 等. Hg²⁺调控氮磷共掺杂碳纳米点在半胱氨酸/同型半胱氨酸荧光检测中的应用[J]. 发光学报, 2022,43(3):440-452. DOI： 10.37188/CJL.20210389.

Lu LU, Lü XU, Xin ZHANG, et al. Fluorescent Detection for Cysteine/Homocysteine Using Hg²⁺ Modulated Nitrogen and Phosphorus Co-doped Carbon Nanodots[J]. Chinese Journal of Luminescence, 2022,43(3):440-452. DOI： 10.37188/CJL.20210389.

摘要

以叶酸和N-(膦酰基甲基)亚氨基二乙酸为原料，制备了具有丰富氮含量(10.0%)和高荧光量子产率(23.64%)的N,P-CDs。由于Hg,2+,与N, P-CDs之间的电子转移作用，N,P-CDs的荧光将被猝灭，基于此构建了半胱氨酸/同型半胱氨酸(Cys/Hcy)增强型纳米探针。利用Hg,2+,和硫醇基团之间更强的相互作用，实现Cys/Hcy的检测。Cys的测定在0.1~150 μmol/L范围内存在线性关系，Hcy的测定在0.1~100 μmol/L范围内存在线性关系，检测限分别为30 nmol/L和50 nmol/L。此外，本文所制备的N,P-CDs具有较强的抗基质干扰能力，可用于自来水和尿液等真实样品检测；因其超低的细胞毒性和出色的细胞渗透性，可用于检测活细胞内的Hg,2+,/Cys，在生物传感方面具有较为广阔的应用前景。

Abstract

A new kind of N,P-CDs with rich nitrogen content(10.0%) and high fluorescence quantum yield(23.64%) was successfully prepared using folic acid and N-(phosphonomethyl)iminodiacetic acid as precursors. Due to the charge transfer between Hg,2+, and N,P-CDs, the fluorescence of N,P-CDs was quenched. Based on this, a cysteine/homocysteine(Cys/Hcy) enhanced nanoprobe was constructed using the stronger interaction between Hg,2+, and -SH. Linear relationships were established within the range of 0.1-150 μmol/L for Cys and 0.1-100 μmol/L for Hcy, respectively. The limit detections of Cys and Hcy were 30 nmol/L and 50 nmol/L. In addition, the as-prepared N,P-CDs with strong anti-matrix interference ability could be used for real samples such as tap water and urine. N,P-CDs were applied to sensing Hg,2+,/Cys within living cells favorably based on their ultralow cytotoxicity and outstanding cell permeability, indicating the potential applications in bioimaging and biosensing.

关键词

NP-CDs荧光探针半胱氨酸检测同型半胱氨酸检测细胞成像

Keywords

N P-CDsfluorescent probescysteine detectionhomocysteine detectioncell image

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Fluorescent Detection for Cysteine/Homocysteine Using Hg2+ Modulated Nitrogen and Phosphorus Co-doped Carbon Nanodots

DOI：10.37188/CJL.20210389

摘要

Abstract

关键词

Keywords

references

Hg²⁺调控氮磷共掺杂碳纳米点在半胱氨酸/同型半胱氨酸荧光检测中的应用

Fluorescent Detection for Cysteine/Homocysteine Using Hg²⁺ Modulated Nitrogen and Phosphorus Co-doped Carbon Nanodots