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1.无锡商业职业技术学院 汽车技术学院,江苏 无锡 214153
2.盐城工学院 材料科学与工程学院,江苏 盐城 224002
[ "路露(1988-),女,江苏无锡人,博士,讲师,2018年于东南大学获得博士学位,主要从事纳米材料的光电研究与应用。E-mail: lulu2143@126.com" ]
纸质出版日期:2022-03,
收稿日期:2021-12-14,
修回日期:2021-12-29,
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路露, 徐律, 张鑫, 等. Hg2+调控氮磷共掺杂碳纳米点在半胱氨酸/同型半胱氨酸荧光检测中的应用[J]. 发光学报, 2022,43(3):440-452.
Lu LU, Lü XU, Xin ZHANG, et al. Fluorescent Detection for Cysteine/Homocysteine Using Hg2+ Modulated Nitrogen and Phosphorus Co-doped Carbon Nanodots[J]. Chinese Journal of Luminescence, 2022,43(3):440-452.
路露, 徐律, 张鑫, 等. Hg2+调控氮磷共掺杂碳纳米点在半胱氨酸/同型半胱氨酸荧光检测中的应用[J]. 发光学报, 2022,43(3):440-452. DOI: 10.37188/CJL.20210389.
Lu LU, Lü XU, Xin ZHANG, et al. Fluorescent Detection for Cysteine/Homocysteine Using Hg2+ Modulated Nitrogen and Phosphorus Co-doped Carbon Nanodots[J]. Chinese Journal of Luminescence, 2022,43(3):440-452. DOI: 10.37188/CJL.20210389.
以叶酸和N-(膦酰基甲基)亚氨基二乙酸为原料,制备了具有丰富氮含量(10.0%)和高荧光量子产率(23.64%)的N
P-CDs。由于Hg
2+
与N
P-CDs之间的电子转移作用,N
P-CDs的荧光将被猝灭,基于此构建了半胱氨酸/同型半胱氨酸(Cys/Hcy)增强型纳米探针。利用Hg
2+
和硫醇基团之间更强的相互作用,实现Cys/Hcy的检测。Cys的测定在0.1~150 μmol/L范围内存在线性关系,Hcy的测定在0.1~100 μmol/L范围内存在线性关系,检测限分别为30 nmol/L和50 nmol/L。此外,本文所制备的N
P-CDs具有较强的抗基质干扰能力,可用于自来水和尿液等真实样品检测;因其超低的细胞毒性和出色的细胞渗透性,可用于检测活细胞内的Hg
2+
/Cys,在生物传感方面具有较为广阔的应用前景。
A new kind of N
P-CDs with rich nitrogen content(10.0%) and high fluorescence quantum yield(23.64%) was successfully prepared using folic acid and N-(phosphonomethyl)iminodiacetic acid as precursors. Due to the charge transfer between Hg
2+
and N
P-CDs
the fluorescence of N
P-CDs was quenched. Based on this
a cysteine/homocysteine(Cys/Hcy) enhanced nanoprobe was constructed using the stronger interaction between Hg
2+
and -SH. Linear relationships were established within the range of 0.1-150 μmol/L for Cys and 0.1-100 μmol/L for Hcy
respectively. The limit detections of Cys and Hcy were 30 nmol/L and 50 nmol/L. In addition
the as-prepared N
P-CDs with strong anti-matrix interference ability could be used for real samples such as tap water and urine. N
P-CDs were applied to sensing Hg
2+
/Cys within living cells favorably based on their ultralow cytotoxicity and outstanding cell permeability
indicating the potential applications in bioimaging and biosensing.
NP-CDs荧光探针半胱氨酸检测同型半胱氨酸检测细胞成像
N P-CDsfluorescent probescysteine detectionhomocysteine detectioncell image
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