基于类芬顿反应的锌掺杂碳量子点荧光探针测定过氧化氢和胆固醇

孙雪花; 强瑜; 郝都婷; 田锐

doi:10.37188/CJL.20210271

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基于类芬顿反应的锌掺杂碳量子点荧光探针测定过氧化氢和胆固醇

发光学应用及交叉前沿 | 更新时间：2021-12-17

- 基于类芬顿反应的锌掺杂碳量子点荧光探针测定过氧化氢和胆固醇
  增强出版
- Detection of Hydrogen Peroxide and Cholest-erol Based on Zinc Doped Quantum Dots and Fenton-like Reaction Fluorescent Probe Preparation
- 发光学报 2021年42卷第12期页码：1951-1960
- 作者机构：
  
  延安大学化学与化工学院　延安市绿色合成材料与化学安全检测重点实验室，陕西　延安　716000
- 作者简介：
  
  [ "孙雪花(1975-)，女，陕西延安人，硕士，副教授，硕士研究生导师，2010 年于延安大学获得硕士学位，主要从事光谱分析方面的研究。E-mail: happyxh908080@163.com" ]
- 基金信息：
  
  陕西省科技厅自然科学基金(2020JQ-789);陕西省教育厅重点科学研究项目(20JS156)
- DOI：10.37188/CJL.20210271
  中图分类号： O657.31
- 纸质出版日期：2021-12，
  
  收稿日期：2021-08-26，
  
  修回日期：2021-09-15，
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孙雪花, 强瑜, 郝都婷, 等. 基于类芬顿反应的锌掺杂碳量子点荧光探针测定过氧化氢和胆固醇[J]. 发光学报, 2021,42(12):1951-1960.

Xue-hua SUN, Yu QIANG, Du-ting HAO, et al. Detection of Hydrogen Peroxide and Cholest-erol Based on Zinc Doped Quantum Dots and Fenton-like Reaction Fluorescent Probe Preparation[J]. Chinese Journal of Luminescence, 2021,42(12):1951-1960.
孙雪花, 强瑜, 郝都婷, 等. 基于类芬顿反应的锌掺杂碳量子点荧光探针测定过氧化氢和胆固醇[J]. 发光学报, 2021,42(12):1951-1960. DOI： 10.37188/CJL.20210271.

Xue-hua SUN, Yu QIANG, Du-ting HAO, et al. Detection of Hydrogen Peroxide and Cholest-erol Based on Zinc Doped Quantum Dots and Fenton-like Reaction Fluorescent Probe Preparation[J]. Chinese Journal of Luminescence, 2021,42(12):1951-1960. DOI： 10.37188/CJL.20210271.

摘要

通过一步水热法合成了荧光量子产率高达34%的锌掺杂碳量子点(Zinc doped carbon quantum dots

Zn-CQDs)，并对Zn-CQDs的结构性能进行分析。基于Cu

与H

产生羟基自由基可有效猝灭Zn-CQDs，构建了Zn-CQDs-Cu

荧光探针检测过氧化氢和胆固醇浓度。实验发现，在pH=7.60的N-2-羟乙基哌嗪-N'-2-乙磺酸(HEPES)缓冲溶液中，于50 ℃孵化40 min时，体系的(

-F

)

与H

浓度在1.0×10

-5

~1.0×10

-6

mol/L范围内，与胆固醇浓度在3.0×10

-5

~9.0×10

-7

mol/L范围内分别呈良好的线性关系，检出限分别为7.2×10

-7

mol/L和6.8×10

-7

mol/L。该方法用于水样中H

和牛奶中胆固醇含量的测定，回收率分别可达98.55%~105.4%和98.00%~103.0%，结果满意。该方法也为检测H

生成反应的其他代谢物提供了有效的检测思路。

Abstract

Zinc doped carbon quantum dots (Zn-CQDs) with fluorescence quantum yield up to 34% were synthesized by one step hydrothermal method

and the structural properties of Zn-CQDs were analyzed. Based on the fact that hydrogen peroxide produced by Cu

and H

can effectively quench Zn-CQDs

a Zn-CQDs-Cu

fluorescent probe was constructed to detect hydrogen peroxide and indirectly detect cholesterol concentration. The effects of buffer type

reaction time and temperature on the determination of H

and cholesterol were investigated. The results showed that (

-F

)

of the system has good linear relationship with the concentration of H

when incubated at 50 ℃ for 40 min in HEPES buffer solution with pH=7.06. The linear range was 1.0×10

-5

~1.0×10

-6

mol/L. The detection limit was 7.2×10

-7

mol/L. The (

-F

)

of the system also has good linear relationship with concentration of cholesterol. The linear range was 3.0×10

-5

-9.0×10

-7

mol/L

and the detection limit was 6.8×10

-7

mol/L. The method was used for the determination of H

in water samples and cholesterol content in milk

the detection recovery rate can reach 98.55%-105.4% and 98.00%-103.0% respectively

and the result was satisfactory. It can also provide effective detection ideas for the detection of other metabolites in the H

reaction.

关键词

锌掺杂碳量子点过氧化氢胆固醇荧光猝灭

Keywords

zinc doped carbon quantum dotshydrogen peroxidecholesterolfluorescence quenching

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