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延安大学化学与化工学院 延安市绿色合成材料与化学安全检测重点实验室,陕西 延安 716000
[ "孙雪花(1975-),女,陕西延安人,硕士,副教授,硕士研究生导师,2010 年于延安大学获得硕士学位,主要从事光谱分析方面的研究。E-mail: happyxh908080@163.com" ]
收稿日期:2021-08-26,
修回日期:2021-09-15,
纸质出版日期:2021-12
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孙雪花, 强瑜, 郝都婷, 等. 基于类芬顿反应的锌掺杂碳量子点荧光探针测定过氧化氢和胆固醇[J]. 发光学报, 2021,42(12):1951-1960.
Xue-hua SUN, Yu QIANG, Du-ting HAO, et al. Detection of Hydrogen Peroxide and Cholest-erol Based on Zinc Doped Quantum Dots and Fenton-like Reaction Fluorescent Probe Preparation[J]. Chinese journal of luminescence, 2021, 42(12): 1951-1960.
孙雪花, 强瑜, 郝都婷, 等. 基于类芬顿反应的锌掺杂碳量子点荧光探针测定过氧化氢和胆固醇[J]. 发光学报, 2021,42(12):1951-1960. DOI: 10.37188/CJL.20210271.
Xue-hua SUN, Yu QIANG, Du-ting HAO, et al. Detection of Hydrogen Peroxide and Cholest-erol Based on Zinc Doped Quantum Dots and Fenton-like Reaction Fluorescent Probe Preparation[J]. Chinese journal of luminescence, 2021, 42(12): 1951-1960. DOI: 10.37188/CJL.20210271.
通过一步水热法合成了荧光量子产率高达34%的锌掺杂碳量子点(Zinc doped carbon quantum dots
Zn-CQDs),并对Zn-CQDs的结构性能进行分析。基于Cu
2+
与H
2
O
2
产生羟基自由基可有效猝灭Zn-CQDs,构建了Zn-CQDs-Cu
2+
荧光探针检测过氧化氢和胆固醇浓度。实验发现,在pH=7.60的N-2-羟乙基哌嗪-N'-2-乙磺酸(HEPES)缓冲溶液中,于50 ℃孵化40 min时,体系的(
F
0
-F
)
/F
0
与H
2
O
2
浓度在1.0×10
-5
~1.0×10
-6
mol/L范围内,与胆固醇浓度在3.0×10
-5
~9.0×10
-7
mol/L范围内分别呈良好的线性关系,检出限分别为7.2×10
-7
mol/L和6.8×10
-7
mol/L。该方法用于水样中H
2
O
2
和牛奶中胆固醇含量的测定,回收率分别可达98.55%~105.4%和98.00%~103.0%,结果满意。该方法也为检测H
2
O
2
生成反应的其他代谢物提供了有效的检测思路。
Zinc doped carbon quantum dots (Zn-CQDs) with fluorescence quantum yield up to 34% were synthesized by one step hydrothermal method
and the structural properties of Zn-CQDs were analyzed. Based on the fact that hydrogen peroxide produced by Cu
2+
and H
2
O
2
can effectively quench Zn-CQDs
a Zn-CQDs-Cu
2+
fluorescent probe was constructed to detect hydrogen peroxide and indirectly detect cholesterol concentration. The effects of buffer type
pH
reaction time and temperature on the determination of H
2
O
2
and cholesterol were investigated. The results showed that (
F
0
-F
)
/F
0
of the system has good linear relationship with the concentration of H
2
O
2
when incubated at 50 ℃ for 40 min in HEPES buffer solution with pH=7.06. The linear range was 1.0×10
-5
~1.0×10
-6
mol/L. The detection limit was 7.2×10
-7
mol/L. The (
F
0
-F
)
/F
0
of the system also has good linear relationship with concentration of cholesterol. The linear range was 3.0×10
-5
-9.0×10
-7
mol/L
and the detection limit was 6.8×10
-7
mol/L. The method was used for the determination of H
2
O
2
in water samples and cholesterol content in milk
the detection recovery rate can reach 98.55%-105.4% and 98.00%-103.0% respectively
and the result was satisfactory. It can also provide effective detection ideas for the detection of other metabolites in the H
2
O
2
reaction.
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