基于类芬顿反应的锌掺杂碳量子点荧光探针测定过氧化氢和胆固醇

孙雪花; 强瑜; 郝都婷; 田锐

doi:10.37188/CJL.20210271

您当前的位置：

首页 >

文章列表页 >

基于类芬顿反应的锌掺杂碳量子点荧光探针测定过氧化氢和胆固醇

发光学应用及交叉前沿 | 更新时间：2021-12-17

- 基于类芬顿反应的锌掺杂碳量子点荧光探针测定过氧化氢和胆固醇
  增强出版
- Detection of Hydrogen Peroxide and Cholest-erol Based on Zinc Doped Quantum Dots and Fenton-like Reaction Fluorescent Probe Preparation
- 发光学报 2021年42卷第12期页码：1951-1960
- 作者机构：
  
  延安大学化学与化工学院　延安市绿色合成材料与化学安全检测重点实验室，陕西　延安　716000
- 作者简介：
  
  [ "孙雪花(1975-)，女，陕西延安人，硕士，副教授，硕士研究生导师，2010 年于延安大学获得硕士学位，主要从事光谱分析方面的研究。E-mail: happyxh908080@163.com" ]
- 基金信息：
  
  陕西省科技厅自然科学基金(2020JQ-789);陕西省教育厅重点科学研究项目(20JS156)
- DOI：10.37188/CJL.20210271
  中图分类号： O657.31
- 纸质出版日期：2021-12，
  
  收稿日期：2021-08-26，
  
  修回日期：2021-09-15，
- 稿件说明：
移动端阅览
孙雪花, 强瑜, 郝都婷, 等. 基于类芬顿反应的锌掺杂碳量子点荧光探针测定过氧化氢和胆固醇[J]. 发光学报, 2021,42(12):1951-1960.

XUE-HUA SUN, YU QIANG, DU-TING HAO, et al. Detection of Hydrogen Peroxide and Cholest-erol Based on Zinc Doped Quantum Dots and Fenton-like Reaction Fluorescent Probe Preparation. [J]. Chinese journal of luminescence, 2021, 42(12): 1951-1960.
孙雪花, 强瑜, 郝都婷, 等. 基于类芬顿反应的锌掺杂碳量子点荧光探针测定过氧化氢和胆固醇[J]. 发光学报, 2021,42(12):1951-1960. DOI： 10.37188/CJL.20210271.

XUE-HUA SUN, YU QIANG, DU-TING HAO, et al. Detection of Hydrogen Peroxide and Cholest-erol Based on Zinc Doped Quantum Dots and Fenton-like Reaction Fluorescent Probe Preparation. [J]. Chinese journal of luminescence, 2021, 42(12): 1951-1960. DOI： 10.37188/CJL.20210271.

摘要

通过一步水热法合成了荧光量子产率高达34%的锌掺杂碳量子点(Zinc doped carbon quantum dots

Zn-CQDs)，并对Zn-CQDs的结构性能进行分析。基于Cu

与H

产生羟基自由基可有效猝灭Zn-CQDs，构建了Zn-CQDs-Cu

荧光探针检测过氧化氢和胆固醇浓度。实验发现，在pH=7.60的N-2-羟乙基哌嗪-N'-2-乙磺酸(HEPES)缓冲溶液中，于50 ℃孵化40 min时，体系的(

-F

)

与H

浓度在1.0×10

-5

~1.0×10

-6

mol/L范围内，与胆固醇浓度在3.0×10

-5

~9.0×10

-7

mol/L范围内分别呈良好的线性关系，检出限分别为7.2×10

-7

mol/L和6.8×10

-7

mol/L。该方法用于水样中H

和牛奶中胆固醇含量的测定，回收率分别可达98.55%~105.4%和98.00%~103.0%，结果满意。该方法也为检测H

生成反应的其他代谢物提供了有效的检测思路。

Abstract

Zinc doped carbon quantum dots (Zn-CQDs) with fluorescence quantum yield up to 34% were synthesized by one step hydrothermal method

and the structural properties of Zn-CQDs were analyzed. Based on the fact that hydrogen peroxide produced by Cu

and H

can effectively quench Zn-CQDs

a Zn-CQDs-Cu

fluorescent probe was constructed to detect hydrogen peroxide and indirectly detect cholesterol concentration. The effects of buffer type

reaction time and temperature on the determination of H

and cholesterol were investigated. The results showed that (

-F

)

of the system has good linear relationship with the concentration of H

when incubated at 50 ℃ for 40 min in HEPES buffer solution with pH=7.06. The linear range was 1.0×10

-5

~1.0×10

-6

mol/L. The detection limit was 7.2×10

-7

mol/L. The (

-F

)

of the system also has good linear relationship with concentration of cholesterol. The linear range was 3.0×10

-5

-9.0×10

-7

mol/L

and the detection limit was 6.8×10

-7

mol/L. The method was used for the determination of H

in water samples and cholesterol content in milk

the detection recovery rate can reach 98.55%-105.4% and 98.00%-103.0% respectively

and the result was satisfactory. It can also provide effective detection ideas for the detection of other metabolites in the H

reaction.

关键词

锌掺杂碳量子点过氧化氢胆固醇荧光猝灭

Keywords

zinc doped carbon quantum dotshydrogen peroxidecholesterolfluorescence quenching

references

LIN L P, LUO Y X, TSAI P, et al. Metal ions doped carbon quantum dots: synthesis,physicochemical properties,and their applications[J]. TrAC Trends Anal. Chem., 2018, 103:87-101.

ZHU C, YANG S W, SUN J, et al. Deep ultraviolet emission photoluminescence and high luminescece efficiency of ferric passivated graphene quantum dots:strong negative inductive effect of Fe[J]. Synth. Met., 2015, 209:468-472.

LIN X, XU D, ZHAO R, et al. Highly efficient photocatalytic activity of g-C3N4 quantum dots (CNQDs)/Ag/Bi2MoO6 nanoheterostructure under visible light[J]. Sep. Purif. Technol., 2017, 178:163-168.

WANG L, WANG Y T, SUN X F, et al. Versatile self-assembly and biosensing applications of DNA and carbon quantum dots coordinated cerium ions[J]. Chem-Eur. J., 2017, 23(43):10413-10422.

MENG A L, XU Q H, ZHAO K, et al. A highly selective and sensitive “on-off-on” fluorescent probe for detecting Hg(Ⅱ) based on Au/N-doped carbon quantum dots[J]. Sens. Actuators B: Chem., 2018, 255:657-665.

ZHUO S J, GAO L L, ZHANG P, et al. Living cell imaging and sensing of hydrogen sulfide using high-efficiency fluorescent Cu-doped carbon quantum dots[J]. New J. Chem., 2018, 42(24):19659-19664.

DING H, YU S B, WEI J S, et al. Full-color light-emitting carbon dots with a surface-state-controlled luminescence mechanism[J]. ACS Nano, 2016, 10(1):484-491.

XU Q, SU R G, CHEN Y S, et al. Metal charge transfer doped carbon dots with reversibly switchable,ultra-high quantum yield photoluminescence[J]. ACS Appl. Nano Mater., 2018, 1(4):1886-1893.

YUE L L, LI H L, SUN Q, et al. Red-emissive ruthenium-containing carbon dots for bioimaging and photodynamic cancer therapy[J]. ACS Appl. Nano Mater., 2020, 3(1):869-876.

WEI J F, QIANG L, REN J, et al. Fluorescence turn-off detection of hydrogen peroxide and glucose directly using carbon nanodots as probes[J]. Anal. Methods, 2014, 6(6):1922-1927.

LIU J W, LUO Y, WANG Y M, et al. Graphitic carbon nitride nanosheets-based ratiometric fluorescent probe for highly sensitive detection of H2O2 and glucose[J]. ACS Appl. Mater. Interfaces, 2016, 8(49):33439-33445.

QIAN Z S, SHAN X Y, CHAI L J, et al. Si-doped carbon quantum dots:a facile and general preparation strategy,bioimaging application,and multifunctional sensor[J]. ACS Appl. Mater. Interfaces, 2014, 6(9):6797-6805.

满华盛, 吕霞敏, 黄建颖. 壳聚糖基碳量子点的制备、表征及其对过氧化氢的荧光检测研究[J]. 核农学报, 2021, 35(5):1147-1153.

MANH S, LYU X M, HUANG J Y. Preparation and characterization of chitosan-based carbon dots and its application in hydrogen peroxide analysis[J]. J. Nucl. Agric. Sci., 2021, 35(5):1147-1153. (in Chinese)

王令臣, 洪诚毅, 黄志勇. 基于氯化血红素模拟酶活性的荧光分析法检测牛奶中胆固醇含量[J]. 中国食品学报, 2021, 21(5):363-368.

WANG L C, HONG C Y, HUANG Z Y. Fluorescence detection of cholesterol in milk based on hemin as peroxidase mimetic[J]. J. Chin. Inst. Food Sci. Technol., 2021, 21(5):363-368. (in Chinese)

孙雪花, 张锦婷, 郝都婷, 等. 基于Ag+修饰氮掺杂碳量子点用于组氨酸的荧光开启检测[J]. 分析试验室, 2021, 40(4):399-403.

SUN X H, ZHANG J T, HAO D T, et al. Determination of histidine by a signal-on strategy based on Ag+ modified N-doped carbon quantum dots[J]. Chin. J. Anal. Lab., 2021, 40(4):399-403. (in Chinese)

占霞飞, 唐建设, 吴军, 等. 硅掺杂碳量子点荧光猝灭法测定水样中铜(Ⅱ)[J]. 分析测试学报, 2016, 35(11):1461-1465.

ZHAN X F, TANG J S, WU J, et al. Determination of copper ions in water samples by silicon doped carbon quantum dots[J]. J. Instr. Anal., 2016, 35(11):1461-1465. (in Chinese)

任瑞妮, 付柯, 冯巩, 等. 分光光度法测定羟基自由基捕获剂的研究进展[J]. 安徽化工, 2014, 40(3):19-21.

REN R N, FU K, FENG G, et al. Research progress of spectrophotometric method to determine the hydroxyl free radical scavenger[J]. Anhui Chem. Ind., 2014, 40(3):19-21. (in Chinese)

古桃. 铜系催化剂类芬顿氧化法处理染料废水的研究[D]. 郑州: 郑州大学, 2018.

GU T. Study on the Fenton-like Oxidation of Dye Wastewater with Copper Based Catalysts[D]. Zhengzhou: Zhengzhou University, 2018. (in Chinese)

刘迪, 尚华. 基于铜纳米团簇的纳米探针比色法检测牛奶中胆固醇含量[J]. 食品科学, 2014, 35(12):143-147.

LIU D, SHANG H. Copper nanoclusters-based nanoprobes for colorimetric detection of cholesterol in milk[J]. Food Sci., 2014, 35(12):143-147. (in Chinese)

GORASSINI A, VERARDO G, FREGOLENT S C, et al. Rapid determination of cholesterol oxidation products in milk powder based products by reversed phase SPE and HPLC-APCI-MS/MS[J]. Food Chem., 2017, 230:604-610.

LIN T R, ZHONG L S, CHEN H, et al. A sensitive colorimetric assay for cholesterol based on the peroxidase-like activity of MoS2 nanosheets[J]. Microchim. Acta, 2017, 184(4):1233-1237.

ZHU L, XU L L, TAN L, et al. Direct electrochemistry of cholesterol oxidase immobilized on gold nanoparticles-decorated multiwalled carbon nanotubes and cholesterol sensing[J]. Talanta, 2013, 106:192-199.

CHANG H C, HO J A A. Gold nanocluster-assisted fluorescent detection for hydrogen peroxide and cholesterol based on the inner filter effect of gold nanoparticles[J]. Anal. Chem., 2015, 87(20):10362-10367.

浏览量

307

下载量

CSCD

文章被引用时，请邮件提醒。

提交

工具集

关联资源

基于2-（2'-羟基苯基）苯并噻唑衍生物的过氧化氢荧光探针及其合成方法与应用

基于荧光猝灭效应的光纤传感器研究进展

白色荧光粉CaF₂:Yb³⁺/Eu³⁺/La³⁺的制备及其上转换发光特性

β-环糊精修饰Mn-ZnS量子点荧光探针用于检测血液中对乙酰氨基酚