单激发双发射近红外荧光碳量子点制备、荧光性能与细胞成像

湛志华; 陈茺而; 莫大幸; 谢木标; 薛茗月; 韩国成; 毛惠会; 钟菁青

doi:10.37188/CJL.20210157

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单激发双发射近红外荧光碳量子点制备、荧光性能与细胞成像

特邀报告 | 更新时间：2021-09-03

- 单激发双发射近红外荧光碳量子点制备、荧光性能与细胞成像
  增强出版
- Preparation, Fluorescent Properties and Cell Imaging of Near Infrared Fluorescent Carbon Quantum Dots with Single Excited Double Emission
- 发光学报 2021年42卷第8期页码：1307-1313
- 作者机构：
  
  1.岭南师范学院　化学化工学院，广东　湛江　 524048
  2.桂林电子科技大学　生命与环境科学学院，广西　桂林　 541004
- 作者简介：
  
  [ "湛志华(1980-)，男，湖北监利人，博士，2011年于中南大学获得博士学位，主要从事生物传感及天然产物提取与利用方面的研究。E-mail: 517537695@qq.com" ]
  [ "薛茗月(1979-)，女，湖北钟祥人，博士，教授，硕士研究生导师，2016年于广西师范大学获得博士学位，主要从事纳米材料及生物传感方面的研究。E-mail: xmy818@163.com" ]
- 基金信息：
  
  国家自然科学基金(61861010;81873913);广东省教育厅项目(2019KTSCX091);广西自然科学基金(2018JJA120061);岭南师范学院人才专项;稀土资源利用与发光材料研究创新团队(2017KCXTD022)
- DOI：10.37188/CJL.20210157
  中图分类号： O482.31
- 纸质出版日期：2021-08-01，
  
  收稿日期：2021-04-29，
  
  修回日期：2021-05-15，
扫描看全文
湛志华, 陈茺而, 莫大幸, 等. 单激发双发射近红外荧光碳量子点制备、荧光性能与细胞成像[J]. 发光学报, 2021,42(8):1307-1313.

Zhi-hua ZHAN, Chong-er CHEN, Da-xing MO, et al. Preparation, Fluorescent Properties and Cell Imaging of Near Infrared Fluorescent Carbon Quantum Dots with Single Excited Double Emission[J]. Chinese Journal of Luminescence, 2021,42(8):1307-1313.
湛志华, 陈茺而, 莫大幸, 等. 单激发双发射近红外荧光碳量子点制备、荧光性能与细胞成像[J]. 发光学报, 2021,42(8):1307-1313. DOI： 10.37188/CJL.20210157.

Zhi-hua ZHAN, Chong-er CHEN, Da-xing MO, et al. Preparation, Fluorescent Properties and Cell Imaging of Near Infrared Fluorescent Carbon Quantum Dots with Single Excited Double Emission[J]. Chinese Journal of Luminescence, 2021,42(8):1307-1313. DOI： 10.37188/CJL.20210157.

摘要

探索合成方法简单的近红外比率荧光探针具有重要意义。本文以高山榕树叶为碳源，乙醇、丙酮为提取剂，采用溶剂热法于200 ℃高压反应釜中反应10 h，一步合成具有近红外双发射荧光特性的碳量子点(CDs)。透射电镜(TEM)表明合成的CDs分散性好、平均尺寸约为4.70 nm，晶格常数为0.32 nm。X射线衍射(XRD)结果显示该碳点在2

=22°附近有一个较宽的衍射峰，表明该CDs为sp

杂化的无定形碳。傅里叶红外光谱(FT-IR)显示其含有氨基、羰基、醇羟基和甲基。荧光光谱表明，最大激发波长410 nm，最大发射波长为466 nm和676 nm。以硫酸奎宁做参比测得荧光量子产率为26.31%。实验还探讨了CDs的酸碱、光学、抗盐和金属离子的稳定性以及细胞成像效果。以上结果表明，该碳点具有原料廉价易得、制作简便、对酸碱稳定、抗光漂白性良好等优点，具有良好的光学成像应用前景。

Abstract

It is of significance to explore the simple synthetic method and near-infrared ratio fluorescence probe. In this paper

carbon quantum dots(CDs) with near-infrared double-emission fluorescence properties were synthesized by solvothermal reaction in 200 ℃ high pressure reactor for 10 h using Ficus alba leaves as carbon source

ethanol and acetone as extraction agent. Transmission electron microscopy(TEM) showed that the average size of CDs is about 4.70 nm and the lattice constant is 0.32 nm. X-ray diffraction(XRD) results showed there is a wide diffraction peak at near 2

=22°. It showed the CDs was amorphous carbon sp

hybridized. Fourier transform infrared spectroscopy (FT-IR) indicated the CDs had amino

carbonyl

alcohol hydroxyl

and methyl. Fluorescence spectra showed that the maximum excitation wavelength was 410 nm

and the maximum emission wavelength was 466 nm and 676 nm. Using quinine sulfate as a reference

the fluorescence quantum yield was 26.31%.The stability of acid-base

optical

salt resistance and metal ion resistance and the effect of cell imaging were also discussed. The above results show the CDs has the advantages of cheap raw materials

easy to produce

stable to acid and alkali

good photobleaching resistance and so on. It has good prospects for optical imaging applications.

关键词

碳量子点近红外荧光细胞成像

Keywords

carbon quantum dots(CDs)near-infrared fluorescencecell imaging

references

XU X Y, RAY R, GU Y L, et al. Electrophoretic analysis and purification of fluorescent single-walled carbon nanotube fragments [J].J. Am. Chem. Soc., 2004, 126(40): 12736-12737.

SUN Y P, ZHOU B, LIN Y, et al. Quantum-sized carbon dots for bright and colorful photoluminescence [J].J. Am. Chem. Soc., 2006, 128(24): 7756-7757.

张川洲, 谭辉, 毛燕, 等. 发光碳量子点的合成、性质和应用[J].应用化学, 2013, 30(4): 367-372.

ZHANG C Z, TAN H, MAO Y, et al. Synthesis and properties of luminescent carbon dots and its applications [J].Chin. J. Appl. Chem., 2013, 30(4): 367-372. (in Chinese)

SONG Y C, FENG D, SHI W, et al. Parallel comparative studies on the toxic effects of unmodified cdte quantum dots, gold nanoparticles, and carbon nanodots on live cells as well as green gram sprouts [J].Talanta, 2013, 116: 237-244.

MOMPER R, STEINBRECHER J, DORN M, et al. Enhanced photoluminescence properties of a carbon dot system through surface interaction with polymeric nanoparticles [J].J. Colloid Interface Sci., 2018, 518: 11-20.

DA SILVA J C G E, GONÇALVES H M R. Analytical and bioanalytical applications of carbon dots [J].TrACTrends Anal. Chem., 2011, 30(8): 1327-1336.

GONÇALVES H M R, DUARTE A J, DA SILVA J C G E. Optical fiber sensor for Hg(Ⅱ) based on carbon dots [J].Biosens. Bioelectron., 2010, 26(4): 1302-1306.

LIU C J, ZHANG P, ZHAI X Y, et al. Nano-carrier for gene delivery and bioimaging based on carbon dots with PEI-passivation enhanced fluorescence [J].Biomaterials, 2012, 33(13): 3604-3611.

BAJPAIS K, D’SOUZA A, SUHAIL B. Carbon dots from guar gum: synthesis, characterization and preliminary in vivo application in plant cells [J].Mater. Sci. Eng.:B, 2019, 241: 92-99.

FENG T, AI X Z, AN G H, et al. Correction to charge-convertible carbon dots for imaging-guided drug delivery with enhanced in vivo cancer therapeutic efficiency [J].ACS Nano, 2016, 10(5): 5587-5587.

CHANG Q, HAN X J, XUE C R, et al. Cu1.8S-passivated carbon dots for enhancing photocatalytic activity [J].Chem. Commun., 2017, 53(15): 2343-2346.

JIANG K, ZHANG L, LU J F, et al. Triple-mode emission of carbon dots: applications for advanced anti-counterfeiting [J].Angew. Chem. Int. Ed., 2016, 55(25): 7231-7235.

高宁萧, 徐玉龙, 刘勇. 豆奶粉提取碳点及含碳点荧光纳米纤维的制备[J].高等学校化学学报, 2019, 40(3): 555-559.

GAO N X, XU Y L, LIU Y.Preparation of carbon dots from soy milk powder and fluorescent nanofibers containing carbon dots [J].Chem. J. Chin. Univ., 2019, 40(3): 555-559. (in Chinese)

王胜达, 袁楠, 朱志峰, 等. 荧光碳点的合成、性质和应用[J].影像科学与光化学, 2016, 34(3): 203-218.

WANG S D, YUAN N, ZHU Z F, et al. Synthesis, properties and applications of the fluorescent carbon dots [J].Imaging Sci. Photochem., 2016, 34(3): 203-218. (in Chinese)

YANG Z C, WANG M, YONG A M, et al. Intrinsically fluorescent carbon dots with tunable emission derived from hydrothermal treatment of glucose in the presence of monopotassium phosphate [J].Chem. Commun., 2011, 47(42): 11615-11617.

LIU Y L, AI K L, LIU J H, et al. Dopamine-melanin colloidal nanospheres: an efficient near-infrared photothermal therapeutic agent for in vivo cancer therapy [J].Adv. Mater., 2013, 25(9): 1353-1359.

JIANG B P, ZHOU B, SHEN X C, et al. Selective probing of gaseous ammonia using red-emitting carbon dots based on an interfacial response mechanism [J].Chem. Eur. J., 2015, 21(52): 18993-18999.

AMJADI M, HALLAJ T, ASADOLLAHI H, et al. Facile synthesis of carbon quantum dot/silver nanocomposite and its application for colorimetric detection of methimazole [J].Sens. Actuators B:Chem., 2017, 244: 425-432.

KERMIS H R, KOSTOV Y, HARMS P, et al. Dual excitation ratiometric fluorescent pH sensor for noninvasive bioprocess monitoring: development and application [J].Biotechnol. Prog., 2002, 18(5): 1047-1053.

ROYZENM , DAI Z H, CANARY J W. Ratiometric displacement approach to Cu(Ⅱ) sensing by fluorescence [J].J. Am. Chem. Soc., 2005, 127(6): 1612-1613.

ZHOU X F, SU F Y, LU H G, et al. An FRET-based ratiometric chemosensor for in vitro cellular fluorescence analyses of pH [J].Biomaterials, 2012, 33(1): 171-180.

LI G, ZHU D J, XUE L, et al. Quinoline-based fluorescent probe for ratiometric detection of lysosomal pH [J].Org. Lett., 2013, 15(19): 5020-5023.

WANG Q L, ZHENG H Z, LONG Y J, et al. Microwave-hydrothermal synthesis of fluorescent carbon dots from graphite oxide [J].Carbon, 2011, 49(9): 3134-3140.

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