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河北大学化学与环境科学学院 药物化学与分子诊断教育部重点实验室
收稿:2010-10-29,
修回:2010-11-15,
网络首发:2011-03-22,
纸质出版:2011-03-22
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刘保生, 杨超, 王晶, 薛春丽, 吕运开. 硫酸头孢匹罗与牛血清白蛋白结合反应的发光机理[J]. 发光学报, 2011,32(3): 293-299
LIU Bao-sheng, YANG Chao, WANG Jing, XUE Chun-li, LU Yun-kai. Luminescence Mechanism Study of The Conjugation Reaction between Cefpirome Sulfate and Bovine Serum Albumin[J]. Chinese Journal of Luminescence, 2011,32(3): 293-299
刘保生, 杨超, 王晶, 薛春丽, 吕运开. 硫酸头孢匹罗与牛血清白蛋白结合反应的发光机理[J]. 发光学报, 2011,32(3): 293-299 DOI:
LIU Bao-sheng, YANG Chao, WANG Jing, XUE Chun-li, LU Yun-kai. Luminescence Mechanism Study of The Conjugation Reaction between Cefpirome Sulfate and Bovine Serum Albumin[J]. Chinese Journal of Luminescence, 2011,32(3): 293-299 DOI:
在人生理条件下
利用荧光猝灭法、同步荧光法及共振光散射法
分别研究了不同温度下硫酸头孢匹罗(CPS)与牛血清白蛋白(BSA)间的结合反应。结果表明:随着CPS浓度的增加
BSA的荧光、共振散射光依次降低
其荧光猝灭为静态猝灭过程并伴随非辐射能量转移作用。反应的结合常数为10
4
数量级
结合位点数约为1;结合位点位于BSA的亚结构ⅡA中;结合距离
r
<7 nm。CPS与BSA间作用力主要是静电引力;Hill系数
n
H
<1
表明CPS有弱的负协同作用。同步荧光光谱表明CPS对BSA构象产生影响
使BSA腔内疏水环境的极性增强
疏水性减弱。
paper
Mathias U, Jung M. Determination of drug-serum protein interactions via fluorescence polarization measurements [J]. Analytical and Bioanalytical Chemistry, 2007, 388 (5-6):1147-1156.[2] Sun Guanning, Wu Qiongzhu. Content and related substances determination of cefpirome sulfate by HPLC [J]. Pharmaceutical and Clinical Research (药学与临床研究), 2007, 15 (2):152-154 (in Chinese).[3] Bogdam S. Fluorescence study of sinapic acid interaction with bovine serum albumin and egg albumin [J]. J. Fluores-cence, 2003, 13 (4):349-356.[4] Zhang Guowen, Chen Xiuxia, Pan Junhui, et al. Thermodynamic analysis on the binding feature between morin and bovine serum albumin [J]. Journal of Nanchang University (南昌大学学报), 2008, 30 (3):229-233 (in Chinese).[5] Bai Haixin, Yang Cheng, Yang Xiurong. Study on the interaction of bovine serum albumin with Indo-1 by fluorescence method [J]. Chem. J. Chin. Univ. (高等学校化学学报), 2007, 28 (2):227-233 (in Chinese).[6] Steiner R F, Weinryb L. Exicited States of Protein and Nucleic Acid [M]. New York: Plenum Press, 1971:40.[7] Zhang Hongmei, Wang Yanqing, Qiu Jingxia, et al. Studies on the interaction between morin and bovine hemoglobin (BHb) by spectroscopic methods [J]. Chin. J. Lumin. (发光学报), 2007, 28 (4):566-572 (in English).[8] Wang Y Q, Zhang H M, Zhang G C, et al. Interaction of the flavonoid hesperidin with bovine serum albumin [J]. J. Lumin., 2007, 126 (1):211-218.[9] Liu Baosheng, Xue Chunli, Wang Jing, et al. Studies on the interaction between ciprofloxacin and ofloxacin in presence of bovine serum albumin by spectroscopic methods [J]. Chin. J. Lumin. (发光学报), 2010, 31 (2):285-290 (in Chinese).[10] Shao Shuang, Ma Boying, Wang Xuejie, et al. Study on the interaction between cefodizime sodium and bovine serum albumin [J]. Acta Phys. Chim. Sin. (物理化学学报), 2005, 21 (7):792-795 (in Chinese).[11] Gharagozlou M, Boghaei D M. Interaction of water-soluble amino acid Schiff base complexes with bovine serum albumin:fluorescence and circular dichroism studies [J]. Spectrochim. Acta, Part A, 2008, 71 (4):1617-1622.[12] Sukowska A, Bojko B, Rownicka J, et al. Paracetamol and cytarabine binding competition in high affinity binding sites of transporting protein [J]. J. Mol. Struct., 2006, 792-793 :249-256.[13] Sukowska A, Maci ek-Jurczyk M, Bojko B, et al. Competitive binding of phenylbutazone and colchicine to serum albumin in multidrug therapy [J]. J. Mol. Struct., 2008, 881 (1-3):97-106.[14] Maci ek-Jurczyk M, Sukowska A, Bojko B, et al. Fluorescence analysis of competition of phenylbutazone and methotrexate in binding to serum albumin in combination treatment in rheumatology [J]. J. Mol. Struct., 2009, 924-926 :378-384.[15] Liang Hong, Bian Hedong, Tu Chuqiao, et al. Binding equilibrium study between La(Ⅲ) and HAS or BSA [J]. Chem. J. Chin. Univ. (高等学校化学学报), 2001, 22 (1):21-25 (in Chinese).[16] Ilichev Y V, Perry J L, Simon J D, et al. Interaction of ochratoxin a with human serum albumin:preferential binding of the dianion and pH effects [J]. J. Phys. Chem. B, 2002, 106 (2):452-459.[17] Weiss S. Fluorescence spectroscopy of single biomolecules [J]. Science, 1999, 283 (5408):1676-1683.[18] Xu H, Gao S L, Lv J B, et al. Spectroscopic investigations on the mechanism of interaction of crystal violet with bovine serum albumin [J]. J. Mol. Struct., 2009, 919 (1-3):334-338.
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