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1. 天水师范学院 生命科学与化学学院
2. 天水市中医医院
3. 兰州大学 化学化工学院
收稿:2010-06-04,
修回:2010-8-10,
网络首发:2011-02-22,
纸质出版:2011-02-22
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冯小强, 伏国庆, 李小芳, 杨声, 苏中兴. 稀土La(Ⅲ)、Nd(Ⅲ)与牛血清白蛋白的相互作用[J]. 发光学报, 2011,32(2): 205-209
FENG Xiao-qiang, FU Guo-qing, LI Xiao-fang, YANG Sheng, SU Zhong-xing. Interaction of Rare Earth with Bovine Serum Albumin[J]. Chinese Journal of Luminescence, 2011,32(2): 205-209
采用紫外吸收、荧光光谱和红外光谱
研究了稀土离子La(Ⅲ)和Nd(Ⅲ)与牛血清白蛋白(BSA)的相互作用。结果表明: La(Ⅲ)和Nd (Ⅲ)离子能配位诱导BSA
使其分子中色氨酸和酪氨酸芳杂环疏水基团外露
在280 nm处的吸收增强而表现出增色效应和较小的蓝移。La(Ⅲ)和Nd(Ⅲ)可以有规律地猝灭BSA的内源荧光
其猝灭机理是稀土离子与BSA形成复合物的静态猝灭。在20 ℃下
La(Ⅲ)和Nd (Ⅲ)与BSA的结合常数
K
A
分别为8.12
&
times;10
6
和3.85
&
times;10
6
。
paper
Wang Dongmei, Liu Qingyun, Jian Jiangzhuang, et al. Synthesis and optical properties of transparent resins containing rare earth compounds [J]. Chin. J. Lumin. (发光学报), 2009, 30 (4):427-435 (in Chinese).[2] Cui Haixia, Chen Jianmin, Zhou Huidi. Synthesis and fluorescence properties of rare earth complex with a new diamidel ligand [J]. Chin. J. Lumin. (发光学报), 2009, 30 (4):457-462 (in Chinese).[3] Deng Ruwen, Zhang Zhongsheng. Rare earth medicine and pharmacy property [J]. Chin. Rare Earth (稀土), 1987,(3):36-47 (in Chinese).[4] Sugio S, Kashima A, Mochizuki S, et al. Crystal structure of human serum albumin at 2.5 resolution [J]. Protein Eng., 1999, 12 (6):439-446.[5] Liu Baosheng, Xue Chunli, Wang jing, et al. Studies on the interaction between ciprofloxacin and ofloxacin in presence of bovine serum albumin by spectroscopic methods [J]. Chin. J. Lumin. (发光学报), 2010, 31 (2):285-290.[6] Tang Bo, He Xiwen, Shen Hanxi. Analysis of synchronous and derivative synchronous fluorescence spectra of phenol and dihydroxybenzene [J]. Spectroscopy and Spectral Analysis (光谱学与光谱分析), 1998, 18 (1):21-26 (in Chinese).[7] Huang Bo, Zou Guolin, Yang Tianming. Studies on the interaction between adriamycin and bovine serum albumin [J]. Acta Chimica Sinica (化学学报), 2002, 60 (10):1867-1871 (in Chinese).[8] Xuan Guangshan, Wu Xiaonan, Li Yuping. Fluorescence study on the interaction of acetylsalicylic acid and bovine serum albumin [J]. Chin. J. Spectroscopy Laboratory (光谱实验室), 2005, 22 (7):861-864 (in Chinese).[9] Xie Mengxia, Xu Xiaoyun, Wang Yingdian. Spectroscopic investigation of the interaction between 2,3-dihydro- 4',5,7-trihydroxyflavone and human serum albumin [J]. Acta Chimica Sinica (化学学报), 2005, 63 (22):2055-2062 (in Chinese).[10] Chen Guozhen, HuangXianzhi, Zheng Zhuxing. Fluorescence Analytic Technique[M]. Beijing: Science Press, 1990:122.[11] Li Guizhi, Liu Yongming, Guo Xinyun, et al. Study on the interaction between bovine serum albumins and topotecan hydrochloride or irinotecan hydrochloride [J]. Acta Chimica Sinica (化学学报), 2006, 64 (7):679-685 (in Chinese).
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